International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Peripheral Cellular Mechanisms of Artemin-induced Thermal Hyperalgesia in Rats

  • Kim, Hye-Jin (Department of Oral Physiology, School of Dentistry, Kyungpook National University) ;
  • Yang, Kui-Ye (Department of Oral Physiology, School of Dentistry, Kyungpook National University) ;
  • Lee, Min-Kyung (Department of Dental Hygiene, Dong-Eui University) ;
  • Park, Min-Kyoung (Department of Dental Hygiene, Kyung-Woon University) ;
  • Son, Jo-Young (Department of Oral Physiology, School of Dentistry, Kyungpook National University) ;
  • Ju, Jin-Sook (Department of Oral Physiology, School of Dentistry, Kyungpook National University) ;
  • Ahn, Dong-Kuk (Department of Oral Physiology, School of Dentistry, Kyungpook National University)
  • Received : 2017.01.19
  • Accepted : 2017.02.22
  • Published : 2017.03.31
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Abstract

In the present study, we investigated the role of peripheral ionotropic receptors in artemin-induced thermal hyperalgesia in the orofacial area. Male Sprague-Dawley rats weighting 230 to 280 g were used in the study. Under anesthesia, a polyethylene tube was implanted in the subcutaneous area of the vibrissa pad, which enabled drug-injection. After subcutaneous injection of artemin, changes in air-puff thresholds and head withdrawal latency time were evaluated. Subcutaneous injection of artemin (0.5 or $1{\mu}g$) produced significant thermal hyperalgesia in a dose-dependent manner. However, subcutaneous injection of artemin showed no effect on air-puff thresholds. IRTX ($4{\mu}g$), a TRPV1 receptor antagonist, D-AP5 (40 or $80{\mu}g$), an NMDA receptor antagonist, or NBQX (20 or $40{\mu}g$), an AMPA receptor antagonist, was injected subcutaneously 10 min prior to the artemin injection. Pretreatment with IRTX and D-AP5 significantly inhibited the artemin-induced thermal hyperalgesia. In contrast, pretreatment with both doses of NBQX showed no effect on artemin-induced thermal hyperalgesia. Moreover, pretreatment with H-89, a PKA inhibitor, and chelerythrine, a PKC inhibitor, decreased the artemin-induced thermal hyperalgesia. These results suggested that artemin-induced thermal hyperalgesia is mediated by the sensitized peripheral TRPV1 and NMDA receptor via activation of protein kinases.

Keywords

References

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