Anti-cariogenic Properties of α-Pinene, a Monoterpene in Plant Essential Oil

  • Park, Bog-Im (Department. of Oral Biochemistry, School of Dentistry, Wonkwang University) ;
  • You, Yong-Ouk (Department. of Oral Biochemistry, School of Dentistry, Wonkwang University) ;
  • Mo, Ji-Su (Department. of Oral Biochemistry, School of Dentistry, Wonkwang University) ;
  • An, So-Youn (Department of Pediatric dentistry, School of Dentistry, Wonkwang University) ;
  • Choi, Na-Young (Department of Home Economics Education, Wonkwang University) ;
  • Kim, Kang-Ju (Department of Oral Microbiology & Immunology, School of Dentistry, Wonkwang University)
  • Received : 2017.02.21
  • Accepted : 2017.03.10
  • Published : 2017.03.31


Dental caries is the most common chronic disease in the dental field. Streptococcus mutans (S. mutans) is the most important bacteria in the formation of dental plaque and dental caries. In a previous study, we confirmed that the essential oil of Chrysanthemum boreale has antibacterial activity against S. mutans. Alpha-pinene is one of the major chemical components of Chrysanthemum boreale essential oil. In the present study, we investigated the inhibitory effects of ${\alpha}-pinene$ on cariogenic properties such as growth, acid production, biofilm formation, and bactericidal activity on S. mutans. Alpha-pinene at a concentration range of 0.25-0.5 mg/mL significantly inhibited the growth of S. mutans and acid production of S. mutans. Biofilm formation was significantly inhibited at > 0.0625 mg/mL ${\alpha}-pinene$, similar to the data from scanning electronic microscopy. Under confocal laser scanning microscopy, the bacterial viability was decreased by ${\alpha}-pinene$ in a dose-dependent manner. These results suggested that ${\alpha}-pinene$ may be a useful agent for inhibiting the cariogenic properties of S. mutans.


Supported by : 원광대학교


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