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Anti-cariogenic Properties of α-Pinene, a Monoterpene in Plant Essential Oil

  • Park, Bog-Im (Department. of Oral Biochemistry, School of Dentistry, Wonkwang University) ;
  • You, Yong-Ouk (Department. of Oral Biochemistry, School of Dentistry, Wonkwang University) ;
  • Mo, Ji-Su (Department. of Oral Biochemistry, School of Dentistry, Wonkwang University) ;
  • An, So-Youn (Department of Pediatric dentistry, School of Dentistry, Wonkwang University) ;
  • Choi, Na-Young (Department of Home Economics Education, Wonkwang University) ;
  • Kim, Kang-Ju (Department of Oral Microbiology & Immunology, School of Dentistry, Wonkwang University)
  • Received : 2017.02.21
  • Accepted : 2017.03.10
  • Published : 2017.03.31

Abstract

Dental caries is the most common chronic disease in the dental field. Streptococcus mutans (S. mutans) is the most important bacteria in the formation of dental plaque and dental caries. In a previous study, we confirmed that the essential oil of Chrysanthemum boreale has antibacterial activity against S. mutans. Alpha-pinene is one of the major chemical components of Chrysanthemum boreale essential oil. In the present study, we investigated the inhibitory effects of ${\alpha}-pinene$ on cariogenic properties such as growth, acid production, biofilm formation, and bactericidal activity on S. mutans. Alpha-pinene at a concentration range of 0.25-0.5 mg/mL significantly inhibited the growth of S. mutans and acid production of S. mutans. Biofilm formation was significantly inhibited at > 0.0625 mg/mL ${\alpha}-pinene$, similar to the data from scanning electronic microscopy. Under confocal laser scanning microscopy, the bacterial viability was decreased by ${\alpha}-pinene$ in a dose-dependent manner. These results suggested that ${\alpha}-pinene$ may be a useful agent for inhibiting the cariogenic properties of S. mutans.

Acknowledgement

Supported by : 원광대학교

References

  1. Nakagawa M. Evaluation of aroma therapeutic odor compounds on human biological reacition and its application. Aroma Res. 2000;1:30-36.
  2. Kohlert C, van Rensen I, Marz R, Schindler G, Graefe EU, Veit M. Bioavailability and pharmacokinetics of natural volatile terpenes in animals and humans. Planta Med. 2000;66:495-505. doi:10.1055/s-2000-8616. https://doi.org/10.1055/s-2000-8616
  3. Lee, SY, Kim, JG, Baik, BJ, Yang YM, Lee KY, Lee YH, Kim MA. Antimicrobial effect of essential oils on oral bacteria. J Korean Acad Pediatr Dent. 2009;36:1-11.
  4. Williamson MI, Samaranayake LP, MacFarlane TW. Biotypes of oral Candida albicans and Candida tropicalisisolates. J Med Vet Mycol. 1986;24:81-84. https://doi.org/10.1080/02681218680000101
  5. Lee DH, Seo BR, Kim HY, Gum GC, Yu HH, You HK, Kang TH, You YO. Inhibitory effect of Aralia continentalis on the cariogenic properties of Streptococcus mutans. J Ethnopharmcol. 2011;137:979-984. doi: 10.1016/j.jep.2011.07.015. https://doi.org/10.1016/j.jep.2011.07.015
  6. Park YN, Jeong SS, Zeng J, Kim, SH, Hong SJ, Ohk SH, Choi CH. Anti-cariogenic effects of erythritol on growth and adhesion of Streptococcus mutans. Food Sci Biotechnol. 2014;23:1587-1591. doi: 10.1007/s10068-014-0215-0. https://doi.org/10.1007/s10068-014-0215-0
  7. Jeong Sl, Lee Sb, Moon HD, Ra JY, Lee KH, You YO. Inhibitory effect of continetalic acid from Aralia continentalis on Streptococcus mutans biofilm. Int J Oral Biol. 2012;31:177-184.
  8. Jeong SI, Kim BS, Keum KS, Lee KH, Kang SY, Park BI, Lee YR, You YO. Kaurenoic acid from Aralia continentalis Inhibits biofilm formation of Streptococcus mutans. Evid Based Complement Alternat Med. 2013;2013:1-9. doi:10.1155/2013/160592.
  9. Lee SY, Kim JG, Baik BJ, Yang YM, Lee KY, Lee YH, Kim MA. Antimicrobial effect of essential oils on oral bacteria. J Korean Acad Pediatr Dent. 2009;36:1-11.
  10. Lee KH, Kim BS, Keum KS, Yu HH, Kim YH, Chang BS, Ra JY, Moon HD, Seo BR, Choi NY, You YO. Essential oil of Curcuma longa inhibits Streptococcus mutans biofilm formation. J Food Sci. 2011;7:226-230. doi:10.1111/j.1750-3841.2011.02427.x.
  11. Paster BJ, Boches SK, Galvin JL, Ericson RE, Lau CN, Levanos VA, Sahasrabudhe A, Dewhirst FE. Bacterial diversity in human subgingival plaque. J Bacteriol. 2001 ;183:3770-3783. doi: 10.1128/JB.183.12.3770-3783.2001. https://doi.org/10.1128/JB.183.12.3770-3783.2001
  12. Whiley RA, Beighton D. Current classification of the oral streptococci. Oral Microbiol Immunol. 1998;13:195-216. doi:10.1111/j.1399-302x.1998.tb00698.x. https://doi.org/10.1111/j.1399-302X.1998.tb00698.x
  13. Julia D, Hooper SJ, Wilson MJ, Wade WG. Prevotella histicola sp. nov., isolated from the human oral cavity. Int J Syst Evol Microbiol. 2008;58:1788-1791. doi:10.1099/ijs.0.65656-0. https://doi.org/10.1099/ijs.0.65656-0
  14. Rozen R, Bachrach G, Bronshtetyn M, Gedalia I, Steinberg D. The role of fructans on dental biofilm by Streptococcus sobrinus, Streptococcus mutans, Streptococcus gordonii and Actinomyces viscosus. FEMS Microbial Lett. 2001;195: 205-210. doi:https;//doi.org/10.1111/j.1574-6968.2001.tb10522.x. https://doi.org/10.1111/j.1574-6968.2001.tb10522.x
  15. Jang SI, Lee HO, Kim KJ. Oral immunity. Iksan: daehaksa;1988.
  16. Kim JB, Choi YJ. Public oral health. Seoul: komoonsa; 1991.
  17. Struzycka I. The oral microbiome in dental caries. Pol J Microbiol. 2014;63:127-135.
  18. Fears KP, Gonzalez-Begne M, Love CT, Day DE, Koo H. Surface-induced change in the conformation and glucan production of glucosyl transferase adsorbed on saliva-coated hydroxy apatite. Langmuir. 2015;31:4654-4662. doi:10.1021/la504461h. https://doi.org/10.1021/la504461h
  19. Hur SA. The vaccine against dental caries [dissertation]. Gwangju:Chonnam National University; 2010.
  20. Kim KJ, Park BI, Min JH, Chae MS, Lim JY, Son HJ, Lee GH, An SY, Jeon BH, Choi NY, You YO. Inhibitory effect of Galla chinensis extract on cariogenic propeties of Streptococcus mutans. J Physiol & Pathol Korean Med. 2015;29:189-194. https://doi.org/10.15188/kjopp.2015.04.29.2.189
  21. Jung JY, Kim JH, Choi YH, Song GB. Comparison of virulence in xylitol-resistant mutans streptococci exposed to various carbohydrates. J Korean Acad Oral Health. 2011;35:1-9.
  22. Go GS, Kim MS. Coloured medicinal plants of Korea. Seoul: Academy Publishing Co Ltd;1990.
  23. Kim TS. Wildgrass to be like medication. Seoul: Daewon Publishing Co Ltd; 1989.
  24. Jang DS, Yang MS, Park KH. Sesquiterpene lactone from Hemisteptia lyrata. Planta Med. 1998;64:289-90. doi:10. 1055/s-2006-957436. https://doi.org/10.1055/s-2006-957436
  25. Jang DS, Park KH, Choi SO, Nam SH, Yang MS. Antibacterial substances of the flower of Chrysanthemum zawadskii Herbich var. latilobum Kitamura. J Korean Soc Agric Chem Biotechnol. 1997;40:85-88.
  26. Kim BS, Park SJ, Kim MK, Kim YH, Lee SB, Lee KH, Choi NY, Lee YR, Lee YE, You YO. Inhibitory effects of Chrysanthemum boreale essential oil on biofilm formation and virulence factor expression of Streptococcus mutans. Evid Based Complement Alternat Med. 2015;2015:616309.doi: 10.1155/2015/616309.
  27. Oda Y, Hayashi F, Wakita A, Nagatani Y, Okada M. Five-year longitudinal study of dental caries risk associated with Streptococcus mutans and Streptococcus sobrinus in individuals with intellectual disabilities. J Oral Sci. J-Stage. 2016;16-0325. doi.org/10.2334/josnusd.16-0325. https://doi.org/10.2334/josnusd.16-0325
  28. Okada M, Kawamura M, Oda Y, Yasuda R, Kojima T, Kurihara H. Caries prevalence associated with Streptococcus mutans and Streptococcus sobrinus in japanese schoolchildren. Int J Paediatr Dent. 2012;22:342-348. doi:10.1111/j1365-263x.2011.01203.x. https://doi.org/10.1111/j.1365-263X.2011.01203.x
  29. Shahidi F. Antioxidants in food and food antioxidants. Nahrung. 2000;44:158-163. doi:10.1002/1521-3803(20000501)44;3<158::AID-FOOD 158>3.0.co;2-L. https://doi.org/10.1002/1521-3803(20000501)44:3<158::AID-FOOD158>3.0.CO;2-L
  30. Ghasemi Y, Faridi P, Mehregan I, Mohagheghzadeh A. Ferula gummosa fruits: an aromatic antimicrobial agent. Chem Nat Comp. 2005;41:311-314. https://doi.org/10.1007/s10600-005-0138-3
  31. Lee JH, Lee BK, Kim JH, Lee SH, Hong SK. Comparison of chemical compositions and antimicrobial activities of essential oils from three conifer trees; Pinus densiflora, Cryptomeria japonica, and Chamaecyparis obusa. J Microbiol Biotechnol. 2009;19:391-396. PMID: 19420996. https://doi.org/10.4014/jmb.0803.191
  32. Hong EJ, Na KJ, Choi IG, Choi KC, Jeung EB. Antibacterial and antifungal effects of essential oils from coniferous trees. Biol Pharm Bull. 2004;27:863-866. http://doi.org/10.1248/bpb.27.863. https://doi.org/10.1248/bpb.27.863
  33. Kim DS, Lee HJ, Jeon YD, Han YH, Kee JY, Kim HY, Shin HJ, Kang JW, Lee BS, Kim SH, Kim SJ, Park SH, Choi BM, Park SJ, Um JY, Hong SH. Alpha-pinene exhibits anti-Inflammatory activity through the suppression of MAPKs and the NF-${\kappa}B$ pathway in mouse peritoneal macrophages. Am J Chin Med. 2015;43:731-742. http://dx.dor.org/10.1142/s0192415x15500457. https://doi.org/10.1142/S0192415X15500457
  34. Mercier B, Prost J, Prost M. The essential oil of turpentine and its major volatile fraction (alpha-and beta-pinenes): A review. Int J Occup Med Environ Health. 2009;22:331-342. doi:10.2478/v10001-009-0032-5.
  35. El-Readi MZ, Eid HH, Ashour ML, Eid SY, Labib RM, Sporer F, Wink M. Variations of the chemical composition and bioactivity of essential oils from leaves and stems of Liquidambar styraciflua (Altingiaceae). J Pharm Pharmacol. 2013;65: 1653-1663. doi:10.1111/jphp.12/42. https://doi.org/10.1111/jphp.12142
  36. Popovic V, Petrovic S, Tomic M, Stepanovic-Petrovic R, Micov A, Pavlovic-Drobac M, Couladis M and Niketic M. Antinociceptive and anti-edematous activities of the essential oils of two Balkan endemic Laserpitium species. Nat Prod Commun. 2014;9:125-128.
  37. Haffajee AD, Socransky SS. Microbial etiological agents of destructive periodontal diseases. Periodontol. 2000;1994;5:78-111.doi:10.1111/j.1600-0757.1994.tb00020.x. https://doi.org/10.1111/j.1600-0757.1994.tb00020.x
  38. Page RC. The etiology and pathogenesis of periodontitis. Compend Contin Educ Dent. 2002;23:11-14. PMID:12789963.
  39. Gamboa F, Estupinan M, Galindo A. Presence of Streptococcus mutans in saliva and its relationship with dental caries: antimicrobial susceptibility of the isolates. Univ Sci. 2004;9:23-27.
  40. Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils- A review. Food Chem Toxicol. 2008;46:446-475. http://dx.doi.org/10.1016/j.fct.2007.09.106. https://doi.org/10.1016/j.fct.2007.09.106
  41. Lee SY, Kim JK, Baek BJ, Yang YM, Lee KY, Lee YH, Kim MA. Antibacterial effect of essential oils on oral bacteria. J Korean Acad Pediatr Dent. 2009;36:1-11.
  42. Takarada K, Kimizuka R, Takahashi N, Honma K, Okuda K, Kato T. A comparison of the antibacterial efficacies of essential oils against oral pathogens. Oral Microbiol Immunol. 2004; 19:61-64. doi:10.1046/j.0902-0055.2003.00111.x. https://doi.org/10.1046/j.0902-0055.2003.00111.x
  43. Shapiro S, Meier A, Guggenheim B. The antimicrobial activity of essential oils and essential oil components towards oral bacteria. Oral Microbiol Immunol 1994;9:202-208. doi:10.1111/j.1399-302x.1994.tb00059.x. https://doi.org/10.1111/j.1399-302X.1994.tb00059.x
  44. Ouhayoun JP. Penetrating the plaque biofilm: impact of essential oil mouth wash. J Clin Periodontol. 2003;30:10-12. doi:10.1034/j.1600-051x.30.s5.4.x. https://doi.org/10.1034/j.1600-051X.30.s5.4.x
  45. Charles CH, Mostler KM, Bartels LL, Mankodi SM. Comparative antiplaque and antigingivitis effectiveness of a chlorhexidine and an essential oil mouthrinse: 6-month clinical trial. J Clin Periodontol. 2004;31:878-884. doi:10.1111/j.1600-051x.2004.000578.x. https://doi.org/10.1111/j.1600-051X.2004.00578.x
  46. Kim SY, Kim HN, Jun EJ, Kim JB, Jeong SH. The growth inhibitory effect of some vegetable oils on Streptococcus mutans and Lactobacillus casei. J Korean Acad Oral Health. 2016;40:24-30. doi.org/10.11149/jkaoh.2016.40.1.24. https://doi.org/10.11149/jkaoh.2016.40.1.24
  47. Peeters E, Nelis HJ, Coenye T. Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. J Microbiol Methods. 2008;72:157-165. http://dx.doi.org/10.1016/j.minet2007.11.010. https://doi.org/10.1016/j.mimet.2007.11.010