Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Enhancing the Physical Properties and Lifespan of Bacterial Quorum Quenching Media through Combination of Ionic Cross-Linking and Dehydration

  • Lee, Sang Hyun (School of Chemical and Biological Engineering, Seoul National University) ;
  • Lee, Seonki (School of Chemical and Biological Engineering, Seoul National University) ;
  • Lee, Kibaek (School of Chemical and Biological Engineering, Seoul National University) ;
  • Nahm, Chang Hyun (School of Chemical and Biological Engineering, Seoul National University) ;
  • Jo, Sung-Jun (School of Chemical and Biological Engineering, Seoul National University) ;
  • Lee, Jaewoo (School of Chemical and Biological Engineering, Seoul National University) ;
  • Choo, Kwang-Ho (Department of Environmental Engineering, Kyungpook National University) ;
  • Lee, Jung-Kee (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Chung-Hak (School of Chemical and Biological Engineering, Seoul National University) ;
  • Park, Pyung-Kyu (Department of Environmental Engineering, Yonsei University)
  • Received : 2016.11.07
  • Accepted : 2016.12.13
  • Published : 2017.03.28
Download PDF
⟨ Previous Next ⟩

Abstract

Quorum quenching (QQ) bacteria entrapped in a polymeric composite hydrogel (QQ medium) have been successfully applied in membrane bioreactors (MBRs) for effective biofouling control. However, in order to bring QQ technology closer to practice, the physical strength and lifetime of QQ media should be improved. In this study, enforcement of physical strength, as well as an extension of the lifetime of a previously reported QQ bacteria entrapping hollow cylinder (QQ-HC), was sought by adding a dehydration procedure following the cross-linking of the polymeric hydrogel by inorganic compounds like $Ca^{2+}$ and boric acid. Such prepared medium demonstrated enhanced physical strength possibly through an increased degree of physical cross-linking. As a result, a longer lifetime of QQ-HCs was confirmed, which led to improved biofouling mitigation performance of QQ-HC in an MBR. Furthermore, QQ-HCs stored under dehydrated condition showed higher QQ activity when the storage time lasted more than 90 days owing to enhanced cell viability. In addition, the dormant QQ activity after the dehydration step could be easily restored through reactivation with real wastewater, and the reduced weight of the dehydrated media is expected to make handling and transportation of QQ media highly convenient and economical in practice.

Keywords

References

  1. Jen AC, Wake MC, Mikos AG. 1996. Review: hydrogels for cell immobilization. Biotechnol. Bioeng. 50: 357-364. https://doi.org/10.1002/(SICI)1097-0290(19960520)50:4<357::AID-BIT2>3.0.CO;2-K
  2. de-Bashan LE, Bashan Y. 2010. Immobilized microalgae for removing pollutants: review of practical aspects. Bioresour. Technol. 101: 1611-1627. https://doi.org/10.1016/j.biortech.2009.09.043
  3. Hashimoto S, Furukawa K. 1987. Immobilization of activatedsludge by PVA boric-acid method. Biotechnol. Bioeng. 30: 52-59. https://doi.org/10.1002/bit.260300108
  4. Magri A, Vanotti MB, Szogi AA. 2012. Anammox sludge immobilized in polyvinyl alcohol (PVA) cryogel carriers. Bioresour. Technol. 114: 231-240. https://doi.org/10.1016/j.biortech.2012.03.077
  5. Cruz-Ortiz BR, Rios-Gonzalez LJ, Garcia YG, de la Garza JAR, Rodriguez-Martinez J. 2011. Immobilization of Thermomyces lanuginosus lipase in PVA-alginate beads. J. Mex. Chem. Soc. 55: 176-180.
  6. Dave R, Madamwar D. 2006. Esterification in organic solvents by lipase immobilized in polymer of PVA-alginateboric acid. Process Biochem. 41: 951-955. https://doi.org/10.1016/j.procbio.2005.10.019
  7. Idris A, Zain NAM, Suhaimi MS. 2008. Immobilization of Baker's yeast invertase in PVA-alginate matrix using innovative immobilization technique. Process Biochem. 43: 331-338. https://doi.org/10.1016/j.procbio.2007.12.008
  8. Nunes MAP, Vila-Real H, Fernandes PCB, Ribeiro MHL. 2010. Immobilization of naringinase in PVA-alginate matrix using an innovative technique. Appl. Biochem. Biotechnol. 160: 2129-2147. https://doi.org/10.1007/s12010-009-8733-6
  9. Hanaki K, Hirunmasuwan S, Matsuo T. 1994. Protection of methanogenic bacteria from low pH and toxic materials by immobilization using polyvinyl-alcohol. Water Res. 28: 877-885. https://doi.org/10.1016/0043-1354(94)90094-9
  10. Swamy BY, Prasad CV, Reddy CLN, Sudhakara P, Chung I, Subha MCS, Rao KC. 2012. Preparation of sodium alginate/poly(vinyl alcohol) blend microspheres for controlled release applications. J. Appl. Polym. Sci. 125: 555-561. https://doi.org/10.1002/app.36243
  11. Kamoun EA, Kenawy ERS, Tamer TM, El-Meligy MA, Eldin MSM. 2015. Poly (vinyl alcohol)-alginate physically crosslinked hydrogel membranes for wound dressing applications: characterization and bio-evaluation. Arab. J. Chem. 8: 38-47. https://doi.org/10.1016/j.arabjc.2013.12.003
  12. Lee S, Park SK, Kwon H, Lee SH, Lee K, Nahm CH, et al. 2016. Crossing the border between laboratory and field: bacterial quorum quenching for anti-biofouling strategy in an MBR. Environ. Sci. Technol. 50: 1788-1795. https://doi.org/10.1021/acs.est.5b04795
  13. Lee K, Lee S, Lee SH, Kim S-R, Oh H-S, Park P-K, et al. 2016. Fungal quorum quenching: a paradigm shift for energy savings in membrane bioreactor (MBR) for wastewater treatment. Environ. Sci. Technol. 50: 10914-10922. https://doi.org/10.1021/acs.est.6b00313
  14. Lee SH, Lee S, Lee K, Nahm CH, Kwon H, Oh HS, et al. 2016. More efficient media design for enhanced biofouling control in a membrane bioreactor: quorum quenching bacteria entrapping hollow cylinder. Environ. Sci. Technol. 50: 8596-8604. https://doi.org/10.1021/acs.est.6b01221
  15. Lee S, Lee SH, Lee K, Kwon H, Nahm CH, Lee C-H, et al. 2016. Effect of the shape and size of quorum-quenching media on biofouling control in membrane bioreactors for wastewater treatment. J. Microbiol. Biotechnol. 26: 1746-1754. https://doi.org/10.4014/jmb.1605.05021
  16. Yeon KM, Cheong WS, Oh HS, Lee WN, Hwang BK, Lee CH, et al. 2009. Quorum sensing: a new biofouling control paradigm in a membrane bioreactor for advanced wastewater treatment. Environ. Sci. Technol. 43: 380-385. https://doi.org/10.1021/es8019275
  17. Yeon KM, Lee CH, Kim J. 2009. Magnetic enzyme carrier for effective biofouling control in the membrane bioreactor based on enzymatic quorum quenching. Environ. Sci. Technol. 43: 7403-7409. https://doi.org/10.1021/es901323k
  18. Kim HW, Oh HS, Kim SR, Lee KB, Yeon KM, Lee CH, et al. 2013. Microbial population dynamics and proteomics in membrane bioreactors with enzymatic quorum quenching. Appl. Microbiol. Biotechnol. 97: 4665-4675. https://doi.org/10.1007/s00253-012-4272-0
  19. Oh HS, Yeon KM, Yang CS, Kim SR, Lee CH, Park SY, et al. 2012. Control of membrane biofouling in MBR for wastewater treatment by quorum quenching bacteria encapsulated in microporous membrane. Environ. Sci. Technol. 46: 4877-4884. https://doi.org/10.1021/es204312u
  20. Kim SR, Lee KB, Kim JE, Won YJ, Yeon KM, Lee CH, Lim DJ. 2015. Macroencapsulation of quorum quenching bacteria by polymeric membrane layer and its application to MBR for biofouling control. J. Membr. Sci. 473: 109-117. https://doi.org/10.1016/j.memsci.2014.09.009
  21. Kim SR, Oh HS, Jo SJ, Yeon KM, Lee CH, Lim DJ, et al. 2013. Biofouling control with bead-entrapped quorum quenching bacteria in membrane bioreactors: physical and biological effects. Environ. Sci. Technol. 47: 836-842. https://doi.org/10.1021/es303995s
  22. Kose-Mutlu B, Ergon-Can T, Koyuncu I, Lee CH. 2016. Quorum quenching MBR operations for biofouling control under different operation conditions and using different immobilization media. Desalin. Water Treat. 57: 17696-17706. https://doi.org/10.1080/19443994.2015.1086899
  23. Maqbool T, Khan SJ, Waheed H, Lee CH, Hashmi I, Iqbal H. 2015. Membrane biofouling retardation and improved sludge characteristics using quorum quenching bacteria in submerged membrane bioreactor. J. Membr. Sci. 483: 75-93. https://doi.org/10.1016/j.memsci.2015.02.011
  24. Oh HS, Kim SR, Cheong WS, Lee CH, Lee JK. 2013. Biofouling inhibition in MBR by Rhodococcus sp BH4 isolated from real MBR plant. Appl. Microbiol. Biotechnol. 97: 10223-10231. https://doi.org/10.1007/s00253-013-4933-7
  25. Weerasekara NA, Choo KH, Lee CH. 2014. Hybridization of physical cleaning and quorum quenching to minimize membrane biofouling and energy consumption in a membrane bioreactor. Water Res. 67: 1-10. https://doi.org/10.1016/j.watres.2014.08.049
  26. Weerasekara NA, Choo K-H, Lee C-H. 2016. Biofouling control: bacterial quorum quenching versus chlorination in membrane bioreactors. Water Res. 103: 293-301. https://doi.org/10.1016/j.watres.2016.07.049
  27. Chen S, Liu M, Jin S, Wang B. 2008. Preparation of ioniccrosslinked chitosan-based gel beads and effect of reaction conditions on drug release behaviors. Int. J. Pharm. 349: 180-187. https://doi.org/10.1016/j.ijpharm.2007.08.029
  28. Khan S, Ranjha NM. 2014. Effect of degree of cross-linking on swelling and on drug release of low viscous chitosan/ poly(vinyl alcohol) hydrogels. Polym. Bull. 71: 2133-2158. https://doi.org/10.1007/s00289-014-1178-2
  29. Keith LH. 1996. Compilation of EPA's Sampling and Analysis Methods, 2nd Ed. CRC/Lewis Publishers, Boca Raton.
  30. Potts M, Slaughter SM, Hunneke FU, Garst JF, Helm RF. 2005. Desiccation tolerance of prokaryotes: application of principles to human cells. Integr. Comp. Biol. 45: 800-809. https://doi.org/10.1093/icb/45.5.800
  31. Lederberg J. 2000. Encyclopedia of Microbiology, 2nd Ed. Academic Press, San Diego.
  32. Gasol JM, Zweifel UL, Peters F, Fuhrman JA, Hagstrom A. 1999. Significance of size and nucleic acid content heterogeneity as measured by flow cytometry in natural planktonic bacteria. Appl. Environ. Microbiol. 65: 4475-4483.
  33. Mah TF, Pitts B, Pell ock B, Wal ker GC, Stewart PS, O'Tool e GA. 2003. A genetic basis for Pseudomonas aeruginosa biofilm antibiotic resistance. Nature 426: 306-310. https://doi.org/10.1038/nature02122
  34. Sachidanandham R, Gin KYH, Poh CL. 2005. Monitoring of active but non-culturable bacterial cells by flow cytometry. Biotechnol. Bioeng. 89: 24-31. https://doi.org/10.1002/bit.20304
  35. Cheong WS, Kim SR, Oh HS, Lee SH, Yeon KM, Lee CH, Lee JK. 2014. Design of quorum quenching microbial vessel to enhance cell viability for biofouling control in membrane bioreactor. J. Microbiol. Biotechnol. 24: 97-105. https://doi.org/10.4014/jmb.1311.11008
  36. Kitamikado M, Tseng CH, Yamaguchi K, Nakamura T. 1992. Two types of bacterial alginate lyases. Appl. Environ. Microbiol. 58: 2474-2478.
  37. Kawai F, Hu XP. 2009. Biochemistry of microbial polyvinyl alcohol degradation. Appl. Microbiol. Biotechnol. 84: 227-237. https://doi.org/10.1007/s00253-009-2113-6
  38. Smidsrod O, Skjakbraek G. 1990. Alginate as immobilization matrix for cells. Trends Biotechnol. 8: 71-78. https://doi.org/10.1016/0167-7799(90)90139-O

Cited by

  1. Membrane-based technologies for post-treatment of anaerobic effluents vol.1, pp.1, 2017, https://doi.org/10.1038/s41545-018-0021-y
  2. A Study on Membrane Fouling Control by Immobilized Microbial Media for Nitrification in Membrane Bioreactor vol.41, pp.5, 2017, https://doi.org/10.4491/ksee.2019.41.5.272
  3. Micro-patterned membranes with enzymatic quorum quenching activity to control biofouling in an MBR for wastewater treatment vol.592, pp.None, 2017, https://doi.org/10.1016/j.memsci.2019.117365
  4. Preparation of a mesoporous silica quorum quenching medium for wastewater treatment using a membrane bioreactor vol.36, pp.4, 2017, https://doi.org/10.1080/08927014.2020.1749601
  5. Inhibition of Microbial Quorum Sensing Mediated Virulence Factors by Pestalotiopsis sydowiana vol.30, pp.4, 2017, https://doi.org/10.4014/jmb.1907.07030
  6. Eggshell Membrane/Gellan Gum Composite Hydrogels with Increased Degradability, Biocompatibility, and Anti-Swelling Properties for Effective Regeneration of Retinal Pigment Epithelium vol.12, pp.12, 2017, https://doi.org/10.3390/polym12122941
  7. Polymeric Materials Used for Immobilisation of Bacteria for the Bioremediation of Contaminants in Water vol.13, pp.7, 2017, https://doi.org/10.3390/polym13071073