Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Mutant Yeast Strains having Resistance to 1-ethyl-3-methylimidazolium Acetate through a Directed Evolutionary Approach

유도적 돌연변이 유발 방법을 통한 1-ethyl-3-methylimidazolium acetate에 대해 내성을 갖는 돌연변이 효모 선별

  • Lee, Yoo-Jin (Department of Bioengineering and Technology, Kangwon National University) ;
  • Kwon, Deok-Ho (Department of Bioengineering and Technology, Kangwon National University) ;
  • Park, Jae-Bum (Department of Bioengineering and Technology, Kangwon National University) ;
  • Ha, Suk-Jin (Department of Bioengineering and Technology, Kangwon National University)
  • Received : 2017.02.23
  • Accepted : 2017.03.21
  • Published : 2017.03.28
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Abstract

Cellulosic biomass is a renewable source for biofuel production from non-edible biomass. An optimized pretreatment process is required for the efficient utilization of cellulosic biomass. Among various pretreatment processes, the use of ionic liquids has been reported recently. However, the residual ionic liquid after pretreatment acts as an inhibitor of microbial fermentation. Recently, we isolated mutant Saccharomyces cerevisiae strains resistant to the ionic liquid 1-ethyl-3-methylimidazolium acetate ([EMIM][Ac]) by using a directed evolutionary approach. When 3% [EMIM][Ac] was added to a medium containing 80 g/l of glucose, mutants D452-B2 and D452-S3 produced 35.6 g/l and 36.3 g/l of ethanol, respectively, for 18 h while the parental strain (S. cerevisiae D452-2) produced 1.3 g/l of ethanol. Thus, these mutant S. cerevisiae strains might prove advantageous when ionic liquids are used for biofuel production from cellulosic biomass.

목질계 바이오 매스 전처리에 사용되는 ionic liquid는 전처리 후 100% 회수되지 않아 잔존하는 ionic liquid의 독성이 직접적으로 미생물 균주의 생육에 나쁜 영향을 미쳐 에탄올 발효의 수율 및 생산성을 저해하는 문제를 가지고 있다. 본 연구에서는 ionic liquid에 저해를 받지 않으며 높은 ethanol 생산 효율을 가진 균주를 얻고자 유도적 돌연변이 유발 실험을 진행하였다. 선별된 돌연변이 균주 D452-B2와 D452-S3는 3% [EMIM][Ac]가 포함된 배지에서 glucose 소비속도는 $4.5g{\cdot}l^{-1}{\cdot}h^{-1}$와 $4.4g{\cdot}l^{-1}{\cdot}h^{-1}$로 모균주인 S. cerevisiae D452-2 균주에 비해 6배 가량 증가하였으며, ethanol 생산성은 각각 $1.99g{\cdot}l^{-1}{\cdot}h^{-1}$와 $2.0g{\cdot}l^{-1}{\cdot}h^{-1}$로 27배 가량 증가하였다.

Keywords

References

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