Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Synergistic Inhibition of Aronia melanocarpa and Moringa oleifera Seed Extract on Experimental Atopic Dermatitis

아로니아 및 모링가 종자 복합물의 항아토피 상승효과

  • Ki, Hyeon-Hui (Department of Immunology and Institute of Medical Science, Chonbuk National University Medical School) ;
  • Lee, Ji-Hyun (Department of Immunology and Institute of Medical Science, Chonbuk National University Medical School) ;
  • Moon, Kwang-Hyun (Sunchang Research Institute of Health and Longevity) ;
  • Lee, Jeong-Ho (Sunchang Research Institute of Health and Longevity) ;
  • Kim, Dae-Geun (Sunchang Research Institute of Health and Longevity) ;
  • Jeong, Kyung-Ok (Sunchang Research Institute of Health and Longevity) ;
  • Im, So-Yeon (Sunchang Research Institute of Health and Longevity) ;
  • Lee, Young-Mi (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Kim, Dae-Ki (Department of Immunology and Institute of Medical Science, Chonbuk National University Medical School)
  • 기현희 (전북대학교 의과대학 및 의과학연구소) ;
  • 이지현 (전북대학교 의과대학 및 의과학연구소) ;
  • 문광현 ((재)순창건강장수연구소) ;
  • 이정호 ((재)순창건강장수연구소) ;
  • 김대근 ((재)순창건강장수연구소) ;
  • 정경옥 ((재)순창건강장수연구소) ;
  • 임소연 ((재)순창건강장수연구소) ;
  • 이영미 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 김대기 (전북대학교 의과대학 및 의과학연구소)
  • Received : 2016.11.23
  • Accepted : 2017.01.17
  • Published : 2017.03.31
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Abstract

Atopic dermatitis is a chronic, relapsing inflammatory skin disease. This study aimed to investigate the therapeutic benefits of Aronia melanocarpa (AM) and Moringa oleifera seed extract (MO) on experimental atopic dermatitis. We examined the effects of AM or MO and their combination on 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis in BALB/c mice as well as tumor necrosis factor $(TNF)-{\alpha}$ and interferon $(IFN)-{\gamma}-stimulated$ HaCaT keratinocytes. Mice were orally treated with extract during repeated application of DNCB to shaved dorsal skin. Our results show that treatment with AM and MO in combination reduced histological manifestations such as epidermal hyperplasia and inflammatory cell infiltration. Furthermore, it significantly decreased skin thickness and serum immunoglobulin E (IgE) level compared to the AM or MO alone treated group. Combined extract of AM and MO suppressed expression of $TNF-{\alpha}/IFN-{\gamma}-induced$ T helper 2 (Th2) chemokines such as thymus and activation-regulated chemokine and macrophage-derived chemokine. To sum up, combination of AM and MO suppressed the inflammatory response and serum IgE as an indicator of several allergic diseases in DNCB-induced experimental atopic dermatitis and Th2 chemokine expression in HaCaT cells. This result suggests that combination of AM and MO could be a valuable strategy to improve atopic dermatitis.

본 연구는 DNCB로 유도한 아토피 피부염 유사 병변 실험동물 모델을 활용한 in vivo와 피부 각질세포주를 이용한 in vitro 연구를 통하여 아로니아와 모링가 종자 추출물의 아토피 피부염 개선 효능을 분석하였다. 조직학적 검사 결과 아로니아 추출물(AM) 또는 모링가 종자 추출물(MO) 단독 투여 시보다 1:1로 혼합한 복합물(AM-MO)을 투여했을 때 표피 부분의 과증식과 염증세포의 침윤이 감소하였으며, 염증 및 부종을 나타내는 피부 두께를 측정한 결과에서도 추출물 단독으로 투여하였을 때보다 AM-MO 투여 시 DNCB 도포군과 비교하여 유의하게 감소함을 보여주었다. 피부장벽 손상으로 인한 수분 손실에 대해서는 유의한 효과를 나타내지 않았으며, 혈청 IgE 수준은 AM과 MO 각각 단독으로 투여했을 때보다 혼합 투여하였을 때 현저하게 억제되었다. In vitro에서는 피부각질세포주인 HaCaT 세포를 $TNF-{\alpha}$ 및 $IFN-{\gamma}$로 자극하면 발현이 증가하는 Th2 케모카인 TARC 및 MDC에 대하여 mRNA 수준을 분석하였을 때, 이 역시 단독 추출물 처리 시보다 복합물 처리 시 억제 효과가 더 뛰어남을 확인하였다. 종합적으로 DNCB로 유도된 아토피 피부염 in vivo 모델에서 아로니아 및 모링가 종자 추출물은 단독으로 투여하는 것보다 복합물로 투여하였을 때 현저하게 피부 염증 및 혈청 내 IgE 생성을 억제하였으며, 피부각질 세포주를 이용한 in vitro 연구에서도 추출물 단독 처리보다 혼합하여 처리하였을 때 더 유의한 Th2 케모카인 억제 활성을 나타내었다. 이로 인해 아로니아 및 모링가 종자 추출물은 복합적으로 사용하였을 때 상호작용에 의해서 더 효과적인 아토피 피부염 개선 식품 소재로써 활용될 가능성이 높을 것으로 생각된다.

Keywords

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