The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Pathological Status of Pyricularia angulata Causing Blast and Pitting Disease of Banana in Eastern India

  • Received : 2016.08.16
  • Accepted : 2016.11.03
  • Published : 2017.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

Incidence of leaf blast on nursery plants and pitting disease on maturing banana bunches has been recorded in banana plantations during rainy season in Eastern India during 2014 to 2015. Taxonomical identification as well as DNA sequence analysis of the internal transcribed spacer region of fungus isolated from affected tissue culture derived plantlets and fruits confirmed the pathogen to be Pyricularia angulata Hashioka "in both the cases". Koch's postulates were proved on young plantlets as well as on maturing fruits of cv. Grand Naine under simulated conditions. Evolutionary history was inferred and presented for our P. angulata strain PG9001 with GenBank accession no. KU984740. The analysis indicated that the P. angulata is phylogenitically distinct from other related species related to both Pyricularia and Magnaporthe. Detailed symptoms of blast lesions on young leaves, transition leaves, mid rib, petioles, peduncle, maturing bunches, bunch stalks and cushions were documented. Notably, the distinct small pitting spots on maturing bunches reduced the visual appeal of mature fruits. Appearance of pitting symptoms on fruits in relation with age of fruits and their distribution pattern on bunch and fingers was also documented in detail. Further, the roles of transitory leaves, weed hosts, seasonality on disease occurrence have also been documented.

Keywords

References

  1. Bussaban, B., Lumyong, S., Lumyong, P., Seelanan, T., Park, D. C., McKenzie, E. H. and Hyde, K. D. 2005. Molecular and morphological characterization of Pyricularia and allied genera. Mycologia 97:1002-1011. https://doi.org/10.1080/15572536.2006.11832750
  2. Chadha, K. L. 2001. Handbook of horticulture. Indian Council of Agricultural Research, New Delhi, India.
  3. Feakin, S. D. 1971. Pest control in bananas. PANS manual (Volume no. 1). Centre for Overseas Pest Research, Foreign and Commonwealth Office, Overseas Development Administration, London, UK.
  4. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
  5. Halmos, S. 1970. Inoculum sources of Pyricularia grisea, the cause of pitting disease of bananas. Phytopathology 60:183-184. https://doi.org/10.1094/Phyto-60-183
  6. Hashioka, Y. 1971. Notes on Pyricularia I. Three species parasitic to Musaceae, Cannaceae and Zingiberaceae. Trans. Mycol. Soc. Jpn. 12:126-135.
  7. Hashioka, Y. 1972. Fine structure of the rice blast. IX. Scanning electronmicroscopical observations on appressoria of the rice blast fungus and other species of Pyricularia. Res. Bull. Fac. Agric. Gifu Univ. 33:65-73.
  8. Hirata, K., Kusaba, M., Chuma, I., Osue, J., Nakayashiki, H., Mayama, S. and Tosa, Y. 2007. Speciation in Pyricularia inferred from multilocus phylogenetic analysis. Mycol. Res. 111:799-808. https://doi.org/10.1016/j.mycres.2007.05.014
  9. Hoette, S. 1936. Pitting disease of bananas in Australia. Proc. Royal Soc. Vic. 48:90-95.
  10. Horticultural Statistics at a Glance 2015. URL http://agricoop.nic.in/sites/default/files/hortstat_glance.pdf.
  11. Johnston, J. R. 1932. The fruit spot of the banana. Bull. Res. Dep. United Fruit Co., Boston, MA, USA.
  12. Kim, W. G., Kim, C. K. and Lee, E. J. 1987. Banana blast caused by Pyricularia angulata Hashioka. Korean J. Plant Pathol. 3:114-119.
  13. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16:111-120. https://doi.org/10.1007/BF01731581
  14. Kohli, M. M., Mehta, Y. R., Guzman, E., De Viedma, L. and Cubilla, L. E. 2011. Pyricularia blast: a threat to wheat cultivation. Czech J. Genet. Plant Breed. 47:S130-S134. https://doi.org/10.17221/3267-CJGPB
  15. Kumar, S., Stecher, G. and Tamura, K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33:1870-1874. https://doi.org/10.1093/molbev/msw054
  16. Male, M. F., Tan, Y. P., Vawdrey, L. L. and Shivas, R. G. 2011. Elucidation of the taxonomy and pathological status of Pyricularia associated with banana blast in Australia. Australas. Plant Dis. Notes 6:22-25. https://doi.org/10.1007/s13314-011-0008-8
  17. Meredith, D. S. 1963. Pyricularia grisea (Cooke) Sacc. causing pitting disease of bananas in Central America. Ann. Appl. Biol. 52:453-463. https://doi.org/10.1111/j.1744-7348.1963.tb03769.x
  18. Mgonja, M. A., Lenne, J. M., Manyasa, E. and Sreenivasaprasad, S. 2007. Finger millet blast management in East Africa. Creating opportunities for improving production and utilization of finger millet. In: Proceedings of the First International Finger Millet Stakeholder Workshop, Projects R8030 and R8445 UK Department for International Development: Crop Protection Programme, September 13-14, 2005 at Nairobi. International Crops Research Institute for the Semi-Arid Tropics, Patancheru, India.
  19. Notteghem, J. L. 1990. Results and orientation of EC projects on rice resistance to blast. Summa Phytopathol. 16:57-72.
  20. Ou, S. H. 1985. Rice diseases. 2nd ed. Commonwealth Mycological Institute, Kew, Surrey, UK.
  21. Puri, K. D., Shrestha, S. M., Chhetri, G. K. B. and Joshi, K. D. 2009. Leaf and neck blast resistance in tropical rice lines under green house condition. Euphytica 165:523-532. https://doi.org/10.1007/s10681-008-9771-9
  22. Rouman, E. C. 1992. Effect of leaf age on components of partial resistance in rice to leaf blast. Euphytica 63:271-279.
  23. Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.
  24. Sangeetha, G., Debasish, B., Srinivas, P. and Singh, H. S. 2017. Improved and efficient method of isolation of Pyricularia angulata Hashioka causing blast and pitting disease of banana. J. Mycopathol. Res. 54:563-564.
  25. Shafaullah, Khan, M. A., Khan, N. A. and Mahmood, Y. 2011. Effect of epidemiological factors on the incidence of paddy blast (Pyricularia oryzae) disease. Pak. J. Phytopathol. 23: 108-111.
  26. Snowdon, A. L. 1990. A colour atlas of post-harvest diseases and disorders of fruits and vegetables. Volume 1. General introduction and fruits. CRC Press, Boca Raton, FL, USA.
  27. Sonah, H., Deshmukh, R. K., Parida, S. K., Chand, S. and Kotasthane, A. 2009. Morphological and genetic variation among different isolates of Magnaporthe grisea collected from Chhattisgarh. Indian Phytopathol. 62:469-477.
  28. Stover, R. H. 1972. Banana, plantain and abaca diseases. Commonwealth Mycological Institute, Kew, Surrey, UK. 316 pp.
  29. Takan, J. P., Akello, B., Esele, P., Manyasa, E. O., Obilana, A. B., Audi, P. O., Kibuka, J., Odendo, M., Oduori, C. A., Ajanga, S., Bandyopadhyay, R., Muthumeenakshi, S., Coll, R., Brown, A. E., Talbot, N. J. and Sreenivasaprasad, S. 2004. Finger millet blast pathogen diversity and management in East Africa: a summary of project activities and outputs. Int. Sorghum Millets Newsl. 45:66-69.
  30. Takan, J. P., Chipili, J., Muthumeenakshi, S., Talbot, N. J., Manyasa, E. O., Bandyopadhyay, R., Sere, Y., Nutsugah, S. K., Talhinhas, P., Hossain, M., Brown, A. E. and Sreenivasaprasad, S. 2012. Magnaporthe oryzae populations adapted to finger millet and rice exhibit distinctive patterns of genetic diversity, sexuality and host interaction. Mol. Biotechnol. 50: 145-158. https://doi.org/10.1007/s12033-011-9429-z
  31. Tomkins, R. G. 1931. Wastage in banana transport. Trop. Agric. (Trinidad) 8:255-264.
  32. Wardlaw, C. W. 1934. Banana diseases. VI. The nature and occurrence of pitting disease and fruit spots. Trop. Agric. 11:8-13.
  33. Wardlaw, C. W. 1961. Banana diseases, including plantains abaca. Longmans, Green and Company, London, UK.
  34. Wardlaw, C. W. and McGuire, L. P. 1932. Pitting disease of bananas. Its nature and control. Trop. Agric. 9:193-195.
  35. White, T. J., Bruns, T., Lee, S. and Taylor, J. W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications, eds. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 315-322. Academic Press, New York, NY, USA.
  36. Yaegashi, H. and Nishihara, N. 1976. Production of the perfect stage in Pyricularia from cereals and grasses. Ann. Phytopathol. Soc. Jpn. 42:511-515. https://doi.org/10.3186/jjphytopath.42.511
  37. Zhang, N., Zhao, S. and Shen, Q. 2011. A six-gene phylogeny reveals the evolution of mode of infection in the rice blast fungus and allied species. Mycologia 103:1267-1276. https://doi.org/10.3852/11-022