The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Selection of Small Synthetic Antimicrobial Peptides Inhibiting Xanthomonas citri subsp. citri Causing Citrus Canker

  • Choi, Jeahyuk (Department of Biotechnology, Yeungnam University) ;
  • Park, Euiho (Department of Biotechnology, Yeungnam University) ;
  • Lee, Se-Weon (International Technology Cooperation Center, Rural Development Administration) ;
  • Hyun, Jae-Wook (Citrus Research Station, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Baek, Kwang-Hyun (Department of Biotechnology, Yeungnam University)
  • Received : 2015.09.12
  • Accepted : 2016.08.25
  • Published : 2017.02.01
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Abstract

Citrus canker disease decreases the fruit quality and yield significantly, furthermore, emerging of streptomycin-resistant pathogens threatens the citrus industry seriously because of a lack of proper control agents. Small synthetic antimicrobial peptides (AMPs) could be a promising alternative. Fourteen hexapeptides were selected by using positional scanning of synthetic peptide combinatorial libraries. Each hexapeptide showed different antimicrobial spectrum against Bacillus, Pseudomonas, Xanthomonas, and Candida species. Intriguingly, BHC10 showed bactericidal activity exclusively on Xanthomonas citri subsp. citri (Xcc), while BHC7 was none-active exclusively against two Pseudomonas spp. at concentration of $100{\mu}g/ml$ suggesting potential selectivity constrained in hexapeptide frame. Three hexapeptides, BHC02, 06 and 11, showed bactericidal activities against various Xcc strains at concentration of $10{\mu}g/ml$. When they were co-infiltrated with pathogens into citrus leaves the disease progress was suppressed significantly. Further study would be needed to confirm the actual disease control capacity of the selected hexapeptides.

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References

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