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Residual Characteristics of Bistrifluron and Chlorantraniliprole in Strawberry (Fragaria ananassa Duch.) for Establishing Pre-Harvest Residue Limit

생산단계 잔류허용기준 설정을 위한 딸기 중 bistrifluron과 chlorantraniliprole의 잔류 특성 연구

  • Lee, Jae Won (School of Natural Resources and Enviromental Science, College of Agriculture and Life Sciences, Kangwon National University,) ;
  • Kim, Ji Yoon (School of Natural Resources and Enviromental Science, College of Agriculture and Life Sciences, Kangwon National University,) ;
  • Kim, Hee gon (School of Natural Resources and Enviromental Science, College of Agriculture and Life Sciences, Kangwon National University,) ;
  • Hur, Kyung Jin (School of Natural Resources and Enviromental Science, College of Agriculture and Life Sciences, Kangwon National University,) ;
  • Kwon, Chan Hyeok (Food Standard Division, Food Safety Policy Bureau, Ministry of Food and Drug Safety) ;
  • Hur, Jang Hyun (School of Natural Resources and Enviromental Science, College of Agriculture and Life Sciences, Kangwon National University,)
  • 이재원 (강원대학교 농업생명과학대학 환경융합학부) ;
  • 김지윤 (강원대학교 농업생명과학대학 환경융합학부) ;
  • 김희곤 (강원대학교 농업생명과학대학 환경융합학부) ;
  • 허경진 (강원대학교 농업생명과학대학 환경융합학부) ;
  • 권찬혁 (식품의약품안전처 식품안전정책국 식품기준과) ;
  • 허장현 (강원대학교 농업생명과학대학 환경융합학부)
  • Received : 2017.03.01
  • Accepted : 2017.03.25
  • Published : 2017.03.31

Abstract

BACKGROUND: Pesticide residue analysis is essentially required for safety evaluation of agricultural products. Bistrifluron and chlorantraniliprole have been currently considered as potentials to deeply evaluate their residues in agricultural products because they are frequently found in strawberry. This work was performed to investigate the residual patterns of bistrifluron and chlorantraniliprole in strawberry after harvest. METHODS AND RESULTS: Strawberry was treated with bistrifluron and chlorantraniliprole 0, 1, 2, 3, 5, 7 and 10 days before harvest under greenhouse conditions. The strawberry samples were subjected to solvent and solid phase extractions followed by LC-MS/MS analysis. There covery percentages of bistrifluron and chlorantraniliprole for tified in the control samples ranged from approximately 82 to 103% with the method limit of 0.005 mg/kg. The concentrations of bistrifluron and chlorantraniliprole in strawberry samples decreased significantly in 10 days after treatment, giving the safety levels of 0.04 to 0.06 mg/kg at 10 days after application, as considered maximum residue limit. The half-lives of bistrifluron and chlorantraniliprole based on first order kinetics were determined to 6.3 days and 6.4 days, respectively. CONCLUSION: Bistrifluron and chlorantraniliprole are suggested to use in strawberry 10 days before harvest to reach residual safety levels.

본 연구는 딸기 중 bistrifluron 및 chlorantraniliprole을 예산군 포장(포장 1), 논산시 포장(포장 2)에 살포한 후, 약제처리 2시간 후를 0일차로 하여 0, 1, 2, 3, 5, 7, 10일에 시료를 채취하였다. 두 시험농약을 LC-MS/MS를 이용하여 분석 한 후 생물학적 반감기를 산출하고 생산단계 농약 잔류허용기준을 설정하였다. 정량한계는 모두 0.005 mg/kg이었으며, 회수율 분석 결과 bistrifluron의 경우 82.8~103.9%, chlorantraniliprole에서는 82.7~100.2%의 회수율을 보였으며, 10% 미만의 변이계수(Coefficient of variation, C.V.) 값을 만족하였다. 딸기 중 bistrifluron 및 chlorantraniliprole의 초기 잔류량은 포장 1에서 0.16 mg/kg, 0.17 mg/kg이었고, 포장 2에서는 0.15 mg/kg, 0.17 mg/kg으로 MRL(bistrifluron: 0.5 mg/kg, chlorantraniliprole: 1.0 mg/kg)보다 낮은 수준을 보였다. 10일 후 잔류량은 포장 1에서 0.05 mg/kg, 0.04 mg/kg, 포장 2에서는 0.05 mg/kg, 0.06 mg/kg이었으며, 초기농도 대비 66.67%, 76.49% 감소하는 것을 보였다. 잔류량에 따른 잔류감소 회귀식을 산출한 결과 딸기 중 두 약제에 대한 반감기는 bistrifluron에서는 두 포장 모두 6.3일, chlorantraniliprole은 4.7일(포장 1)과 6.4일(포장 2)로 나타났다. 잔류감소 회귀식을 이용하여 산출한 생산단계 잔류허용기준은 bistrifluron과 chlorantraniliprole의 수확 10일전 1.10 mg/kg, 2.29 mg/kg으로 제안하였다.

Keywords

References

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