The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Selection of Biocontrol Agent of Tomato Gray Mold Disease from Flower and Pollinator Hive

토마토 꽃과 수정용 벌집으로부터 잿빛곰팡이병 방제 길항균주 선발

  • Kim, Da-Ran (Department of Plant Medicine, Gyeongsang National University) ;
  • Lee, Jun-Taek (Department of Plant Medicine, Gyeongsang National University) ;
  • Kim, Hye sun (Division of Applied Life Science (BK21 Plus), Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Jeon, Chang Wook (Division of Applied Life Science (BK21 Plus), Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Kwak, Youn-Sig (Division of Applied Life Science (BK21 Plus), Institute of Agriculture & Life Science, Gyeongsang National University)
  • 김다란 (경상대학교 식물의학과) ;
  • 이준택 (경상대학교 식물의학과) ;
  • 김혜선 (경상대학교 농업생명과학연구원) ;
  • 전창욱 (경상대학교 농업생명과학연구원) ;
  • 곽연식 (경상대학교 농업생명과학연구원)
  • Received : 2017.03.09
  • Accepted : 2017.03.22
  • Published : 2017.03.31
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Abstract

Gray mold disease, cause by Botrytis cinerea, occurs severe damage on varieties of fruit and vegetable production, and have no a critical control method. In case of chemicals control, it is a trigger emergence of drug resistance strains due to using them continuously. In addition, the pathogen is difficult to control naturally because it is possible to survive regardless of host status. In this study, microorganisms were isolated from tomato flower and hive samples and in order to select suitable microbial control agents for tomato gray mold disease. During six-months study, we isolated 1,004 isolates from flower and 925 isolates from pollinator hive samples. Among them, 6 strains were selected based on result of antifungal activity test. The selected strains showed not only strong antifungal activity against gray mold pathogen, but also cellulase and protease enzyme activities. The selected strains were identified as Paenibacillus polymyxa. In plant assay, P. polymyxa prevented the gray mold disease occurrence near 75%.

국내에서 생산하는 과채류에서 발생되는 잿빛곰팡이병은 명확한 생물학적 방제 방법으로 제시된 바가 없는 실정이다. 국내에서 사용 및 개발되고 있는 화학적 방제 약제의 경우 약제저항성 병원균의 출현이 지속되고 있는 상황이다. 또한 임의 기생성으로 인해 기주의 여부에 상관 없이 생존이 가능하므로 경종적 방제 또한 어려운 실정이다. 이에 본 연구에서는 토마토 꽃에 정착력이 우수한 길항미생물을 선발하기 위해 시설재배지의 토마토 화기와 벌집 시료로 미생물을 분리하여 방제균을 선발하였다. 총 6개월의 토마토 재배기간 중 꽃에서 1,004개 균주, 벌집에서 925균주를 분리하여, 잿빛곰팡이 병원균을 억제력이 우수한 6균주를 선발하였다. 선발된 6균주는 잿빛곰팡이 병원균에 대한 항균활성이 우수할 뿐 아니라, cellulase와 protease의 활성 또한 우수한 것으로 검증되었다. 선발된 균주는 Paenibacillus polymyxa 로 동정 되었으며, 토마토의 잿빛곰팡이 발병률을 약 75% 감소시키는 효과를 나타내었다.

Keywords

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