Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Evaluation of the cosmeceutical activity of ethanol extracts from Perilla frutescens var. acuta

자소엽 에탄올 추출물의 향장학적 활성 평가

  • Park, Do-Young (Interdisciplinary Program of Perfume and Cosmetics,Graduate School of Chonnam National University) ;
  • Lee, Ki-Young (Interdisciplinary Program of Perfume and Cosmetics,Graduate School of Chonnam National University)
  • 박도영 (전남대학교 대학원 향장품학협동과정) ;
  • 이기영 (전남대학교 대학원 향장품학협동과정)
  • Received : 2016.12.08
  • Accepted : 2017.03.10
  • Published : 2017.03.31
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Abstract

The purpose of this study was to investigate the antioxidative and anti-inflammatory activities of ethanol extracts from Perilla frutescens var. acuta by varying the concentration of ethanol at 30, 50, 70, and 90% to utilize the effective extract of Perilla frutescens as a cosmetic and pharmaceutical material. In the DPPH antioxidant activity test, the 70% ethanol extract showed the highest activity with an IC50 of 680.98ppm. ABTS showed a high activity in the 50% ethanol extract and the 70% ethanol extract with an IC50 of 646.94 and 661.94 ppm, respectively. Each ethanol extract showed antioxidant activity at a certain concentration (100-10000 ppm), but did not show any significant relationship with the ethanol extract concentration. In RAW 264.7 macrophages induced by LPS, each of the ethanol extracts showed reduced NO production in all extracts, and more than 50% ethanol extract (10000ppm) inhibited nitric oxide formation by 85% or more. In particular, the 70% ethanol extract showed 90% or more nitric oxide production inhibition. In addition, the MTT assay showed no cytotoxicity at all concentrations (1250-10000 ppm) of each extract. In this study, the ethanol extract of Perilla frutescens var. acuta has antioxidant activity and anti-inflammatory activity that is dependent on the concentration at each extraction concentration.

본 연구는 향장품 소재로 물이 IC50 680.98ppm으로 가장 높은 활성을 나타내었다. ABTS를 이용한 활성 산소 소거능에서는 50% 에탄올 추출물과 70% 에탄올 추출물에서 각각 IC50 646.94, IC50 661.94ppm으로 높은 활성을 보였다. 각 에탄올 추출물은 일정농도(100-10000ppm)에서 항산화 활성을 보였으나 에탄올 추출 농도와는 유의적인 관계를 보이지 않았다. LPS로 염증이 유도된 RAW 264.7 macrophage에 각각의 에탄올 추출물을 처리한 경우 모든 추출물에서 NO생성을 감소시키는 것을 확인 할 수 있었다. 50% 이상의 에탄올 추출물(10000ppm)에서 85%이상의 nitric oxide 생성 억제율을 보였으며, 특히 70% 에탄올 추출물에서 90% 이상의 nitric oxide 생성 억제율을 보였다. 또한 MTT assay를 실시한 결과 각 추출물의 모든 농도에서(1250-10000ppm) 세포독성이 없음을 확인 할 수 있었다. 본 연구를 통해 자소엽 에탄올 추출물은 세포독성이 없는 천연물 유래의 소재로서 각 추출농도에서 농도 의존적으로 항산화 활성과 항염활성을 가짐을 확인할 수 있었다.

Keywords

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