Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Preventive effect of fermented black ginseng against cisplatin-induced nephrotoxicity in rats

  • Jung, Kiwon (College of Pharmacy, CHA University) ;
  • An, Jun Min (GINSENG BY PHARM Co., Ltd.) ;
  • Eom, Dae-Woon (Department of Pathology, University of Ulsan College of Medicine, Gangneung Asan Hospital) ;
  • Kang, Ki Sung (College of Korean Medicine, Gachon University) ;
  • Kim, Su-Nam (Natural Products Research Institute, Korea Institute of Science and Technology)
  • Received : 2015.09.18
  • Accepted : 2016.03.02
  • Published : 2017.04.15
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Abstract

Background: Fermented black ginseng (FBG) is processed ginseng by the repeated heat treatment and fermentation of raw ginseng. The protective effect and mechanism of FBG on cisplatin-induced nephrotoxicity was investigated to evaluate its therapeutic potential. Methods: The free radical scavenging activity of FBG was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH). In addition, the protective effect against cisplatin-induced renal damage was tested in rats. FBG was orally administered every day at a dose of 150 mg/kg body weight for 10 d, and a single dose of cisplatin was administered intraperitoneally (7.5 mg/kg body weight) with 0.9% saline on the $4^{th}$ d. Results: The DPPH radical-scavenging activity of FBG ($IC_{50}=384{\mu}g/mL$) was stronger than that of raw ginseng. The improved DPPH radical-scavenging activity was mediated by the generation phenolic compounds. The decreased cell viability by cisplatin was recovered significantly after treatment with FBG in a dose-dependent manner. Then, the protective effect of FBG on cisplatin-induced oxidative renal damage was investigated in rats. The decreased creatinine clearance levels, which are a reliable marker for renal dysfunction in cisplatin-treated rats, were reduced to the normal level after the administration of FBG. Moreover, FBG showed protective effects against cisplatin-induced oxidative renal damage in rats through the inhibition of $NF-{\kappa}B/p65$, COX-2, and caspase-3 activation. Conclusion: These results collectively show that the therapeutic evidence for FBG ameliorates the nephrotoxicity via regulating oxidative stress, inflammation, and apoptosis.

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References

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