Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
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Salt-water Processing-dependent Change in Anti-oxidative and Anti-inflammatory Effects of Cortex Eucommiae

염수초 포제법에 따른 두충의 항산화 및 항염증 활성 변화 비교연구

  • Koh, Wonil (Department of Korean Medicine Rehabilitation, Jaseng Hospital of Korean Medicine) ;
  • Lee, Jinho (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation) ;
  • Ha, In-Hyuk (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation) ;
  • Chung, Hwa-Jin (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation) ;
  • Lee, In-Hee (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation) ;
  • Lee, Jae-Woong (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation) ;
  • Kim, Eun Jee (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation) ;
  • Gang, Byeong-Gu (Department of Korean Medicine Rehabilitation, Jaseng Hospital of Korean Medicine) ;
  • Jeon, Se Hwan (Department of Korean Medicine Rehabilitation, Jaseng Hospital of Korean Medicine) ;
  • Cho, Yongkyu (Department of Korean Medicine Rehabilitation, Jaseng Hospital of Korean Medicine) ;
  • Kim, Min-Jeong (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation)
  • 고원일 (자생한방병원 한방재활의학과) ;
  • 이진호 (자생의료재단 자생척추관절연구소) ;
  • 하인혁 (자생의료재단 자생척추관절연구소) ;
  • 정화진 (자생의료재단 자생척추관절연구소) ;
  • 이인희 (자생의료재단 자생척추관절연구소) ;
  • 이재웅 (자생의료재단 자생척추관절연구소) ;
  • 김은지 (자생의료재단 자생척추관절연구소) ;
  • 강병구 (자생한방병원 한방재활의학과) ;
  • 전세환 (자생한방병원 한방재활의학과) ;
  • 조용규 (자생한방병원 한방재활의학과) ;
  • 김민정 (자생의료재단 자생척추관절연구소)
  • Received : 2017.03.22
  • Accepted : 2017.04.05
  • Published : 2017.04.30
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Abstract

Objectives The present study aimed to investigate the change in marker compounds, anti-oxidative and anti-inflammatory effects of salt-water processed Cortex Eucommiae. Methods To evaluate the influence of processing on anti-oxidant effect of Cortex Eucommiae, changes in total phenol, total flavonoid, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) free radical scavenging, and ferric reducing antioxidant power (FRAP) between processed and raw Cortex Eucommiae were assessed. In addition, nitrite assay was conducted to determine the influence of processing on anti-inflammatory effect of Cortex Eucommiae. Cell viability was also examined as to elucidate whether processing affects cytotoxicity of Cortex Eucommiae. Finally, high-performance liquid chromatography (HPLC) analysis was conducted to monitor changes in pinoresinol diglucoside amount of processed and raw Cortex Eucommiae. Results Salt-water processed Cortex Eucommiae showed higher total phenol and flavonoid amount, compared to raw Cortex Eucommiae. Furthermore, anti-oxidative activity of processed Cortex Eucommiae was improved as discovered in DPPH, ABTS, and FRAP assays. Anti-inflammatory effect of Cortex Eucommiae was also enhanced following salt-water processing, as evidenced in nitrite assay. HPLC analysis found that the amount of pinoresinol diglucoside, widely known as the marker compound of Cortex Eucommiae, increases through salt-water processing. All experiments were performed with non-toxic concentration of Cortex Eucommiae; processing did not affect the cytotoxicity of Cortex Eucommiae up to the currently adopted concentration. Conclusions The present results support that salt-water processing of Cortex Eucommiae is beneficial in terms of marker compound amount, anti-oxidative, and anti-inflammatory activities. Additional investigations are needed to standardize the processing method of Cortex Eucommiae.

Keywords

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