한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제46권4호
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  • Pages.409-416
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  • 2017
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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LPS로 자극한 대식세포에서 1,2,3,4,6-Penta-O-Galloyl-β-D-Glucose의 염증 억제 효과

Anti-Inflammatory Effects of 1,2,3,4,6-Penta-O-Galloyl-β-D-Glucose in LPS-Stimulated Macrophages

  • 이희원 (대구대학교 식품영양학과) ;
  • 강예림 (대구대학교 식품영양학과) ;
  • 배민서 (대구대학교 식품영양학과) ;
  • 김윤희 (대구대학교 식품영양학과)
  • Lee, Hee Won (Department of Food and Nutrition, College of Engineering, Daegu University) ;
  • Kang, Ye Rim (Department of Food and Nutrition, College of Engineering, Daegu University) ;
  • Bae, Min Seo (Department of Food and Nutrition, College of Engineering, Daegu University) ;
  • Kim, Yoon Hee (Department of Food and Nutrition, College of Engineering, Daegu University)
  • 투고 : 2017.01.18
  • 심사 : 2017.02.23
  • 발행 : 2017.04.30
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초록

천연물 유래 물질의 항염증 활성에 대한 잠재성을 평가하기 위한 일환으로 오배자에서 분리한 PGG가 LPS로 자극한 마우스 대식세포인 RAW264.7 세포에서 염증반응에 미치는 영향에 대해 평가하고 관련 메커니즘에 대해 검토하였다. PGG는 LPS 자극에 의해 유도된 iNOS 및 COX-2 단백질 발현량을 감소함으로써 NO와 $PGE_2$ 생성을 억제할 뿐만 아니라 IL-6, TNF-${\alpha}$와 같은 pro-inflammatory cytokine의 분비를 억제하였다. 이러한 효과는 전사인자인 NF-${\kappa}B$의 세포질에서 핵으로의 이동을 억제함으로써 나타나는 것으로 판단된다. 이러한 결과로부터 PGG가 염증 반응을 저해하는 효과가 있는 것으로 나타나 향후 염증성 질환을 예방, 개선 및 치료하는 데 유용한 물질로 사용될 가능성이 있을 것으로 생각된다.

1,2,3,4,6-Penta-O-galloyl-${\beta}$-D-glucose (PGG) is a gallotannin isolated from Galla Rhois. In a previous study, PGG was shown to suppress the allergic response by attenuating immunoglobulin E production both in vitro and in vivo. However, the effect of PGG on bacteria-induced inflammation at physiological concentration remains unclear. Therefore, the aim of this study was to investigate the effect of PGG on lipopolysaccharide (LPS)-stimulated macrophages. PGG inhibited release of nitric oxide (NO) and prostaglandin $E_2$ by alleviating protein expression of inducible NO synthase and cyclooxygenase-2 in LPS-treated RAW264.7 cells. Furthermore, PGG suppressed the release of interleukin-6 and tumor necrosis factor-${\alpha}$ induced by LPS. Further study indicated that PGG blocked translocation of the p65 subunit of nuclear factor-${\kappa}B$ from the cytosol into the nucleus, which is one of the underlying mechanisms of the anti-inflammatory action of PGG. Collectively, these data suggest that PGG might be useful for the treatment of inflammatory disease.

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