Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Extracts of Five Species of Korean Native Forest Plants on Lipid Accumulation and Reactive Oxygen Species Production during Differentiation of 3T3-L1 Preadipocytes

3T3-L1 세포분화 중 지방축적 및 활성산소종 생성에 대한 국내 산림자원 5종 추출물의 효과

  • Choi, Sun-Il (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Lee, Jong Seok (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Lee, Sarah (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Lee, Hye Jin (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Yeo, Joohong (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Cho, Bong-Yeon (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Lee, Jin-Ha (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Kim, Jae-Min (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Jung, Tae-Dong (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Choi, Seung-Hyun (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Kim, Jong-Yea (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Kang, Suk-Nam (Department of Animal Resource, Daegu University) ;
  • Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
  • 최선일 (강원대학교 식품생명공학과) ;
  • 이종석 (국립생물자원관 유용자원분석과) ;
  • 이사라 (국립생물자원관 유용자원분석과) ;
  • 이혜진 (국립생물자원관 유용자원분석과) ;
  • 여주홍 (국립생물자원관 유용자원분석과) ;
  • 조봉연 (강원대학교 식품생명공학과) ;
  • 이진하 (강원대학교 식품생명공학과) ;
  • 김재민 (강원대학교 식품생명공학과) ;
  • 정태동 (강원대학교 식품생명공학과) ;
  • 최승현 (강원대학교 식품생명공학과) ;
  • 김종예 (강원대학교 식품생명공학과) ;
  • 강석남 (대구대학교 동물자원학과) ;
  • 이옥환 (강원대학교 식품생명공학과)
  • Received : 2017.01.12
  • Accepted : 2017.02.09
  • Published : 2017.04.30
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Abstract

This study investigate the effects of extracts of five species of Korean native forest plants on lipid accumulation and reactive oxygen species (ROS) production during the differentiation of 3T3-L1 cells. Our results show that Korean native forest plants extracts significantly reduced lipid accumulation and ROS production during adipogenesis in 3T3-L1 cells. Especially, Rubus coreanus Miq. was most effective in the inhibition of lipid accumulation and ROS production at a concentration of $100{\mu}g/mL$. Moreover, Rubus coreanus Miq. extracts significantly inhibited adipocyte differentiation, which is dependent on down-regulation of peroxisome proliferator-activated receptor gamma, CCAAT/enhancer-binding protein alpha, and adipocyte-specific fatty acid binding protein, a key adipogenic transcription factor. Therefore, these results suggest that Rubus coreanus Miq. might be a valuable source of bioactive compounds with anti-adipogenic activity.

본 연구는 국내 산림자생 식물 추출물 5종(밤나무, 진달래, 산수유, 복분자딸기, 갈참나무)에 대한 지방세포 분화 억제 및 활성산소종(ROS) 생성 억제 효능을 평가하였다. 지방세포 분화 중 세포 내 지방축적 및 ROS 생성량을 비교한 결과, 국내 산림 식물 5종 모두 지방축적량 및 ROS 생성량을 농도 유의적으로 감소키는 것으로 나타났다. 특히 복분자딸기 추출물을 처리한 지방세포에서 지방 축적 및 ROS 저감에 가장 크게 나타났으며 양성대조군으로 사용된 N-acetyl-L-cysteine과 유사한 결과를 나타내었다. 또한, 복분자딸기 추출물을 처리함으로써 지방세포의 분화를 조절하는 전사인자인 $PPAR{\gamma}$, $C/EBP{\alpha}$ 및 aP2 발현이 억제됨을 확인하였다. 국내 산림 식물 5종 추출물은 천연물에서 유래한 항비만 및 항산화 활성 기능성식품 소재로 활용 가능할 것으로 생각한다.

Keywords

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