한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제32권2호
  • /
  • Pages.147-153
  • /
  • 2017
  • /
  • 1229-1153(pISSN)
  • /
  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
권호 표지
DOI QR코드

DOI QR Code

농업환경에 서식하는 파리에서 분리된 E. coli의 병원성 유전자 및 항생제 내성 조사

Virulence Profile and Antimicrobial Resistance of Escherichia coli from Flies Captured from Agricultural Environment

  • 윤보현 (국립농업과학원 농산물안전성부 유해생물팀) ;
  • 장윤정 (국립농업과학원 농산물안전성부 유해생물팀) ;
  • 김연록 (국립농업과학원 농산물안전성부 유해생물팀) ;
  • 김황용 (농촌진흥청 기술협력국 국제기술협력과) ;
  • 김원일 (국립농업과학원 농산물안전성부 유해생물팀) ;
  • 한상현 (국립농업과학원 농산물안전성부 유해생물팀) ;
  • 김세리 (국립농업과학원 농산물안전성부 유해생물팀) ;
  • 류재기 (국립농업과학원 농산물안전성부 유해생물팀) ;
  • 김현주 (국립농업과학원 농산물안전성부 유해생물팀)
  • Yun, Bohyun (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institute of Agricultural Sciences (NAS), Rural Development Administration (RDA)) ;
  • Jang, Youn Jung (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institute of Agricultural Sciences (NAS), Rural Development Administration (RDA)) ;
  • Kim, Yeon Rok (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institute of Agricultural Sciences (NAS), Rural Development Administration (RDA)) ;
  • Kim, Hwang-Yong (Technology Cooperation Bureau, RDA) ;
  • Kim, Won-Il (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institute of Agricultural Sciences (NAS), Rural Development Administration (RDA)) ;
  • Han, Sanghyun (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institute of Agricultural Sciences (NAS), Rural Development Administration (RDA)) ;
  • Kim, Se-Ri (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institute of Agricultural Sciences (NAS), Rural Development Administration (RDA)) ;
  • Ryu, Jae-Gee (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institute of Agricultural Sciences (NAS), Rural Development Administration (RDA)) ;
  • Kim, Hyun Ju (Microbial Safety Team, Agro-Food Safety & Crop Protection Department, National Institute of Agricultural Sciences (NAS), Rural Development Administration (RDA))
  • 투고 : 2016.12.21
  • 심사 : 2017.03.27
  • 발행 : 2017.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 다양한 농업 환경에서 채집된 파리의 분비물에서 E. coli을 분리하고 분리된 E. coli의 병원성유전자 및 항생제내성을 조사하기 위하여 수행되였다. 파리는 과일농장(n = 19), 장류생산농장(n = 9), 생활쓰레기 야적장(n = 46), 축사(n = 66), 도축장(n = 38), 퇴비장(n = 10)에서 총 188마리를 채집하여 토사물과 배설물로부터 E. coli을 분리 및 동정하였다. 그 결과, 채집된 파리의 63%(119/188)에서 E. coli이 검출되었으며 특히 도축장에서 채집된 파리에서 E. coli의 검출률이 89%(34/38)로 가장 높았다. 또한 분리된 E. coli을 대상으로 병원성 유전자 8종(ST, LT, VT1, VT2, aggR, bfpA, eaeA, ipaH)을 조사한 결과, 도축장에서 채집된 파리에서 분리된 E. coli 중 91%(31/34)가 장독소를 생산할 수 있는 ST유전자를 보유하고 있었다. 분리된 E. coli의 16%(31/188)가 1종 이상의 항생제에 내성을 보였다. 특히, 항생제 사용빈도가 높은 축사에서 채집된 파리의 E. coli 경우에는 59%(23/39)가 항생제 내성을 나타내었다. 분리된 항생제 내성 E. coli 균주 중 10%(12/119)는 2종 이상의 항생제에 내성을 보였고, 모두 축사 채집 파리에서 분리된 균주였으며, 이 중 2개 균주는 다재내성의 지표인 ESBL (Extended-spectrum beta-lactamase)에 양성을 나타내었다. ESBL 양성균주 중 1 균주는 7종의 항생제에 내성을 보였이는 것으로 조사되었다. 본 연구의 결과, 축산환경 서식 파리에서 분리된 E. coli은 병원성 유전자를 보유하고 있을 가능성이 높을 뿐만 아니라 항생제에 내성을 나타낼 가능성도 높기 때문에 농식품을 생산하는 농장이나 식품공장은 가급적 축산환경으로부터 일정한 거리를 두거나 방충망 등의 차단조치가 필요할 것으로 판단된다.

The purpose of this study was to isolate Escherichia coli from flies and to assess pathogenic genes and antibiotic resistance of the isolates. A total of 188 flies were captured in agricultural environment including fruits farms (n = 19), fermented soybean farms (n = 9), municipal waste (n = 46), livestock farms (n = 66), slaughterhouses (n = 38), and manure ground (n = 10). E. coli isolates of captured flies were tested for pathogenic gene and antibiotic resistance using PCR methods and VITEK2 systems. As a result, E. coli from 63% (119/188) of the captured flies has been detected, and the detection rate of E. coli was the highest (89%, 31/34) in flies captured at particular slaughterhouse. Of the 34 isolates, 94% (32/34) were pathogenic gene (ST gene) positive. Twenty-six percent (31/119) of the E. coli isolates were observed being resistant to one or more antibiotics. Markedly, one of E. coli isolates from Livestock farms was resistant to 7 antibiotics including ampicillin, ampicillin/sulbactam, cefazolin, cefotaxime, gentamicin, levofloxacin, and trimethoprim/sulfamethoxazole. In addition, it was ESBL positive. The results of the present study may suggest a risk of transmission of pathogenic and antimicrobial resistant bacteria from flies to livestock environment Therefore, it may need to prevent introducing flies into the agricultural production environment for safe food production.

키워드

참고문헌

  1. Forster, M., Klimpel, S., Mehlhorn, H., Sievert, K., Messler, S., Pfeffer, K.: Pilot study on synanthropic flies (eg Musca, Sarcophaga, Calliphora, Fannia, Lucilia, Stomoxys) as vectors of pathogenic microorganisms. Parasitol. Res., 101, 243-246 (2007). https://doi.org/10.1007/s00436-007-0522-y
  2. Graczyk, T.K., Knight, R., Gilman, R.H., Cranfield, M.R.: The role of non-biting flies in the epidemiology of human infectious diseases. Microb. Infect., 3, 231-235 (2001). https://doi.org/10.1016/S1286-4579(01)01371-5
  3. Moon, R.D.: Muscid flies (Muscidae). Med. Vet. Entomol., 279-301 (2002).
  4. Gupta, A.K., Nayduch, D., Verma, P., Shah, B., Ghate, H.V., Patole, M.S., Shouche, Y.S.: Phylogenetic characterization of bacteria in the gut of house flies (Musca domestica L.). FEMS Microbiol. Ecol., 79, 581-593 (2012). https://doi.org/10.1111/j.1574-6941.2011.01248.x
  5. Echeverria, P., Harrison, B., Tirapat, C., McFarland, A.: Flies as a source of enteric pathogens in a rural village in Thailand. Appl. Environ. Microbiol., 46, 32-36 (1983).
  6. Rochon, K., Lysyk, T., Selinger, Persistence, L.: Persistence of Escherichia coli in immature house fly and stable fly (Diptera: Muscidae) in relation to larval growth and survival. J. Med. Entomol., 41, 1082-1089 (2004). https://doi.org/10.1603/0022-2585-41.6.1082
  7. Kobayashi, M., Sasaki, T., Saito, N., Tamura, K., Suzuki, K., Watanabe, H., Agui, N.: Houseflies: not simple mechanical vectors of enterohemorrhagic Escherichia coli O157: H7. Am. J. Trop. Med. Hyg., 61, 625-629 (1999). https://doi.org/10.4269/ajtmh.1999.61.625
  8. Choo, H., Kim, H., Lee, D., Park, Y.: Microbial control of fly maggots with entomopathogenic nematodes and fungus in outhouses of farmhouses. Korean J. Appl. Entomol., (Korea Republic) (1996).
  9. Gestmann, F., Forster, M., Mehlhorn, H., Sievert, K., Messler, S., Neuhausen, N., Petersdorf, S., Pfeffer, K.: Flies as vectors of microorganisms potentially inducing severe diseases in humans and animals. Arthropods as vectors of emerging diseases: Springer Berlin Heidelberg, pp. 195-226 (2012).
  10. Wales, A., Carrique-Mas, J., Rankin, M., Bell, B., Thind, B., Davies, R.: Review of the carriage of zoonotic bacteria by arthropods, with special reference to Salmonella in mites, flies and litter beetles. Zoonoses Public Health, 57, 299-314 (2010).
  11. Olsen, A.R., Hammack, T.S.: Isolation of Salmonella spp. from the housefly, Musca domestica L., and the dump fly, Hydrotaea aenescens (Wiedemann) (Diptera: Muscidae), at caged-layer houses. J. Food Prot., 63, 958-960 (2000). https://doi.org/10.4315/0362-028X-63.7.958
  12. Iwasa, M., Makino, S.I., Asakura, H., Kobori, H., Morimoto, Y.: Detection of Escherichia coli O157: H7 from Musca domestica (Diptera: Muscidae) at a cattle farm in Japan. J. Med. Entomol., 36, 108-112 (1999). https://doi.org/10.1093/jmedent/36.1.108
  13. Wasala, L., Talley, J.L., DeSilva, U., Fletcher, J., Wayadande, A.: Transfer of Escherichia coli O157: H7 to spinach by house flies, Musca domestica (Diptera: Muscidae). Phytopathology, 103, 373-380 (2013). https://doi.org/10.1094/PHYTO-09-12-0217-FI
  14. Butler, J.F., Garcia-Maruniak, A., Meek, F., Maruniak, J.E.: Wild Florida house flies (Musca domestica) as carriers of pathogenic bacteria. Fla. Entomol., 93, 218-223 (2010). https://doi.org/10.1653/024.093.0211
  15. Nmorsi, O., Agbozele, G., Ukwandu, N.: Some aspects of epidemiology of filth flies: Musca domestica, Musca domestica vicina, Drosophilia melanogaster and associated bacteria pathogens in Ekpoma, Nigeria. Vector Borne Zoonotic Dis., 7, 107-117 (2007). https://doi.org/10.1089/vbz.2006.0539
  16. Khalil, K., Lindblom, G.B., Mazhar, K., Kaijser, B.: Flies and water as reservoirs for bacterial enteropathogens in urban and rural areas in and around Lahore, Pakistan. Epidemiol. Infect., 113, 435-444 (1994). https://doi.org/10.1017/S0950268800068448
  17. Lee, J.I., Han, G.Y., Park, H.H.: Characteristics and antibiotics susceptibility of Escherichia coli isolated from fishery products. Korean J. Food Nutr., 16, 111-115 (2003).
  18. Kim, H.T., Jung, K.T., Lee, D.S., Lee, K.W.: Study on antimicrobial resistance of Escherichia coli isolated from domestic beef on sale (2). Korean J. Vet. Serv., 32, 93-102 (2009).
  19. Jo, M.J., Jeong, A.R., Kim, H.J., Lee, N.R., Oh, S.W., Kim, Y.J., Chun, H.S., Koo, M.S.: Microbiological quality of fresh-cut produce and organic vegetables. Korean J. Food Sci. Technol., 43, 91-97 (2011). https://doi.org/10.9721/KJFST.2011.43.1.091
  20. Yoo, J., Hwang, J.S., Goo, T.W., Yun, E.Y.: Comparative analysis of nutritional and harmful components in Korean and Chinese mealworms (Tenebrio molitor). J. Korean Soc. Food Sci. Nutr., 42, 249-254 (2013). https://doi.org/10.3746/jkfn.2013.42.2.249
  21. Chung, M.Y., Gwon, E.Y., Hwang, J.S., Goo, T.W., Yun, E.Y.: Analysis of general composition and harmful material of Protaetia brevitarsis. J. Life Sci., 23, 664-668 (2013). https://doi.org/10.5352/JLS.2013.23.5.664
  22. Kim, E.M., Lim, J.H., Chang, Y.J., An, S.H., Ahn, M.Y.: Changes in the quality characteristics of cricket (Gryllus bimaculatus) under various processing conditions. Korean J. Food Preserv., 22, 218-224 (2015). https://doi.org/10.11002/kjfp.2015.22.2.218
  23. Azambuja, P., Garcia, E.S., Ratcliffe, N.A.: Gut microbiota and parasite transmission by insect vectors. Trends Parasitol., 21, 568-572 (2005). https://doi.org/10.1016/j.pt.2005.09.011
  24. Moriya, K., Fujibayashi, T., Yoshihara, T., Matsuda, A., Sumi, N., Umezaki, N., Kurahashi, H., Agui, N., Wada, A., Watanabe, H.: Verotoxin-producing Escherichia coli O157: H7 carried by the housefly in Japan. Med. Vet. Entomol., 13, 214-216 (1999). https://doi.org/10.1046/j.1365-2915.1999.00161.x
  25. Alam, M.J., Zurek, L.: Association of Escherichia coli O157: H7 with houseflies on a cattle farm. Appl. Environ. Microbiol., 70, 7578-7580 (2004). https://doi.org/10.1128/AEM.70.12.7578-7580.2004
  26. Rahuma, N., Ghenghesh, K., Ben Aissa, R., Elamaari, A.: Carriage by the housefly (Musca domestica) of multiple-antibiotic-resistant bacteria that are potentially pathogenic to humans, in hospital and other urban environments in Misurata, Libya. Ann. Trop. Med. Parasitol., 99, 795-802 (2005). https://doi.org/10.1179/136485905X65134
  27. Ahmad, A., Ghosh, A., Schal, C., Zurek, L.: Insects in confined swine operations carry a large antibiotic resistant and potentially virulent enterococcal community. BMC microbiol., 11, 23 (2011). https://doi.org/10.1186/1471-2180-11-23
  28. Du, B., Long, Y., Liu, H., Chen, D., Liu, D., Xu, Y., Xie, X.: Extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae bloodstream infection: risk factors and clinical outcome. Intensive Care Med., 28, 1718-1723 (2002). https://doi.org/10.1007/s00134-002-1521-1
  29. Jeong, K.O., Heo, J.H., Lee, J.M., Yun, I.R., Choi, Y.J., Kim, J.S.: Surveillance of antimicobial resistance ratio of E. coli and Enterococcus spp. isolated from fecal and carcasses of pigs in slaughterhouse. Korean J. Vet. Serv., 33, 241-248 (2010).
  30. Kim, A.R., Cho, Y.M., Lim, S.K., Her, M., Jeong, W.S., Jung, S.C., Kown, J.H.: Antimicrobial resistance of commensal bacteria isolated from food-producing animals 3. Antimicrobial resistance of Escherichia coli and Enterococcus spp. isolated from chicken faecal samples. Korean J. Vet. Public Health., 31, 41-49 (2007).
  31. Lim, S.K., Lee, H.S., Byun, J.R., Park, S.Y., Jung, S.C.: Antimicrobial resistance of commensal bacteria isolated from food-producing animals 1. Antimicrobial resistance of Escherichia coli and Enterococcus spp. isolated from cattle faecal samples. Korean J. Vet. Public Health., 31, 21-29 (2007).