Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Inhibition of growth and biofilm formation of Staphylococcus aureus by corosolic acid

Corosolic acid에 의한 Staphylococcus aureus의 생장 및 생물막 형성 저해

  • Yum, Su-Jin (Department of Food Science and Technology, Chungnam National University) ;
  • Kim, Seung Min (Department of Human Ecology, Korea National Open University) ;
  • Yu, Yeon-Cheol (Department of Food Science and Technology, Chungnam National University) ;
  • Jeong, Hee Gon (Department of Food Science and Technology, Chungnam National University)
  • 염수진 (충남대학교 식품공학과) ;
  • 김승민 (한국방송통신대학교 생활과학과) ;
  • 유연철 (충남대학교 식품공학과) ;
  • 정희곤 (충남대학교 식품공학과)
  • Received : 2016.11.21
  • Accepted : 2017.01.13
  • Published : 2017.04.30
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Abstract

Staphylococcus aureus is a pathogenic bacterium that causes food poisoning, exhibits a strong capacity to form biofilm, and is highly resistant to antimicrobial agents. The purpose of this study was to investigate the antimicrobial characteristics of corosolic acid against S. aureus. S. aureus showed high susceptibility to corosolic acid in a concentration-dependent manner. The minimum inhibitory concentration and colony-forming ability determined by the broth microdilution method showed that corosolic acid had strong antimicrobial activity against the bacteria. The diameters of the inhibition zone and numbers of colony forming units at each concentration of corosolic acid were also measured. In addition, corosolic acid displayed potent biofilm inhibition activity against S. aureus at concentrations below its minimum inhibitory concentration. These results suggest that corosolic acid can be used to effectively prevent biofilm formation by S. aureus, thereby making S. aureus more susceptible to the action of antimicrobials.

본 연구에서는 지금까지 거의 보고된 바 없는 corosolic acid의 항균활성에 대해 대표적 식중독균 중 하나인 S. aureus를 대상으로 연구하였다. 대수기의 S. aureus에 corosolic acid를 처리하여 생장 저해 여부를 확인하였고, broth micro-dilution 방법과 agar disc diffusion 방법을 통해 corosolic acid의 S. aureus에 대한 항균활성을 측정해본 결과, corosolic acid의 농도에 비례하여 S. aureus 생장이 저해되는 것으로 확인되었다. 또한 corosolic acid의 존재 조건에서 S. aureus의 바이오필름 형성능이 MIC 보다 높은 농도뿐 아니라 낮은 농도에서 역시 저해되는 것으로 관찰되었다. 따라서 천연물질인 corosolic acid는 S. aureus에 의한 식품 오염을 억제하고 식중독 예방 및 잠재적 치료제로서 개발 될 가능성이 있는 것으로 판단되며, 나아가 다른 종류의 식중독균들에 대한 적용 가능 연구가 필요할 것으로 사료된다.

Keywords

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