Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Protective effects of red ginseng treated with gold nanoparticles against H2O2-induced oxidative stress in neuronal PC-12 cells

금 나노입자를 처리한 홍삼의 산화스트레스 완화 및 PC-12 신경세포 보호

  • Kim, Ji Won (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Cho, Chi Heung (Department of Food Science and Biotechnology, Kyung Hee University) ;
  • Hwang, Yun-Gu (SMNANOBIO Co., Ltd) ;
  • Park, Woo Jung (Department of Marine Food Science and Technology, Gangneung-Wonju National University) ;
  • Kang, Hee (Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim, Dae-Ok (Department of Food Science and Biotechnology, Kyung Hee University)
  • 김지원 (경희대학교 식품생명공학과) ;
  • 조치흥 (경희대학교 식품생명공학과) ;
  • 황윤구 ((주)에스엠나노바이오) ;
  • 박우정 (강릉원주대학교 해양식품공학과) ;
  • 강 희 (경희대학교 동서의학대학원) ;
  • 김대옥 (경희대학교 식품생명공학과)
  • Received : 2016.11.11
  • Accepted : 2017.02.03
  • Published : 2017.04.30
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Abstract

Red ginseng prepared from fresh 6-year-old ginseng treated with colloidal gold nanoparticles was extracted using hot water to evaluate its total phenolic and flavonoid contents, antioxidant capacity, and neuroprotective effects. Water extract of red ginseng treated with gold nanoparticles (WERGGN) had total phenolic and total flavonoid contents of 212.2 mg gallic acid equivalents/$^{\circ}Bx$ and 3.5 mg catechin equivalents/$^{\circ}Bx$, respectively. The antioxidant capacities of WERGGN measured using ABTS, DPPH, and ORAC assays were 272.3, 141.2, and 868.4 mg vitamin C equivalents/$^{\circ}Bx$, respectively. The WERGGN showed protective effects on the viability of neuron-like PC-12 cells against oxidative stress induced by hydrogen peroxide in a dose-dependent manner, partly because of a reduction in intracellular oxidative stress. Acetylcholinesterase and butyrylcholinesterase, which degrade the neurotransmitter acetylcholine to terminate neurotransmission, were inhibited by treatment with WERGGN. These results suggest that WERGGN is useful as a functional material to decrease oxidative stress and neuronal damage.

본 연구에서는 금 나노입자 용액을 생장하는 6년근 인삼에 직접 시비해서 금 나노입자가 전이된 황금인삼을 열수 추출하여 총페놀 함량, 총플라보노이드 함량, 산화방지능 및 신경세포 보호능을 평가하였다. $1^{\circ}Bx$의 황금홍삼 추출물은 총페놀과 총플라보노이드 함량이 각각 212.2 mg GAE와 3.5 mg CE였다. ABTS, DPPH 및 ORAC 법으로 측정시, 황금홍삼 추출물의 산화방지능은 각각 272.3, 141.2, $868.4mg\;VCE/^{\circ}Bx$였다. 황금홍삼 추출물은 과산화수소로부터 유래한 세포 내 산화스트레스를 감소시켜 PC-12 신경세포의 생존율을 농도 의존적으로 증가시켰다. 또한, 황금홍삼 추출물은 신경전달물질인 아세틸콜린을 가수분해하는 AChE 및 BChE 활성을 억제하였다. 이러한 결과는 금 나노입자를 처리한 홍삼을 이용한 산화방지 및 신경손상억제 소재로 활용할 가능성을 제시하였다.

Keywords

References

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