DOI QR코드

DOI QR Code

Quality characteristics and biological activity of Yanggaeng with Glechoma hederacea var. longituba Nakai powder

긴병꽃풀의 생리활성과 이를 첨가한 양갱의 품질특성

  • Lim, Su-Bin (School of Food science and Biotechnology/Food and Bio-industry Research Institute, Kyungpook National University) ;
  • Park, Ki-Tae (School of Culinary Art and Baking technology, Dongju College University) ;
  • Lee, Eun-Ho (School of Food science and Biotechnology/Food and Bio-industry Research Institute, Kyungpook National University) ;
  • Kim, Byung-Oh (School of Food science and Biotechnology/Food and Bio-industry Research Institute, Kyungpook National University) ;
  • Lee, Seon-Ho (Department of Food Science and technology, Yeungnam University) ;
  • Kang, In-Kyu (Department of Horicultural Science, Kyungpook National University) ;
  • Cho, Young-Je (School of Food science and Biotechnology/Food and Bio-industry Research Institute, Kyungpook National University)
  • 임수빈 (경북대학교 식품공학부/식품생물산업연구소) ;
  • 박기태 (동주대학교 외식조리제과계열) ;
  • 이은호 (경북대학교 식품공학부/식품생물산업연구소) ;
  • 김병오 (경북대학교 식품공학부/식품생물산업연구소) ;
  • 이선호 (영남대학교 식품가공학과) ;
  • 강인규 (경북대학교 원예과학과) ;
  • 조영제 (경북대학교 식품공학부/식품생물산업연구소)
  • Received : 2016.11.22
  • Accepted : 2017.03.27
  • Published : 2017.04.30

Abstract

The objective of this study is to investigate the quality characteristics of Yanggaeng by using the functional properties of Glechoma hederacea (GH). Sample was dried at $50^{\circ}C$ dry oven. The results of the study were as follows : The Phenolic compounds of GH was $12.99{\pm}0.3mg/g$ in water extract (GHWE), $3.14{\pm}0.07mg/g$ in 70% ethanol extract (GHEE). The antioxidant activity of GH was determined in various phenolic concentrations at $50-200{\mu}g/mL$. DPPH activities of GHWE and GHEE were 77.16-78.24% and 73.04-77.00%, respectively. The ABTS were 84.35-99.75% and 83.74-99.55%. The anti-oxidant protection factor (PF) were 1.54-1.62 PF and 1.62-2.09 PF and TBARS were 42.93-94.09% and 91.05-95.19%, respectively. Tyrosinase inhibitory activity of GHEE increased concentration dependently. Hyaluronidase inhibition activity of GHEE and GHWE, showing that there were increasing pattern depending on the increases in the phenolics concentration of GH. In texture, Hardness and springiness were significantly different in the control and 2% groups, but cohesiveness and chewiness did not show any significant difference. In color, L value decreased in proportion to concentration, and a and b values did not change. Sensory characteristics showed that the 1% group had the highest score and the 2% group had the lowest score. Thus, when the GHP Yanggaeng was prepared, in consideration of its sensory characteristics, and at appropriate concentration on below 1%.

본 연구에서는 긴병꽃풀의 항산화 효과와 기능성을 활용한 양갱을 제조하여 기계적 특성을 측정하고, 관능적 기호도 평가를 실시하였다. 생리활성 물질의 양을 측정하기 위하여 페놀성 화합물 정량을 실시한 결과 긴병꽃풀 열수추출물에서 $12.99{\pm}0.30mg/g$, 70% ethanol 추출물에서 $3.14{\pm}0.07mg/g$으로 나타내었다. $50-200{\mu}g/mL$의 농도에서 항산화 효과를 측정한 결과, 전자공여능은 열수추출물과 70% ethanol 추출물에서 각각 77.16-78.24%, 73.04-77.00%로 나타내었으며, ABTS는 84.35-99.75%, 83.74-99.55%로 나타내었다. Antioxidant protection factor(PF)는 1.54-1.62, 1.62-2.09 PF를 나타내었으며, TBARs는 42.93-94.09%, 91.05-95.19%로 나타내었다. 긴병꽃풀 추출물의 열수추출물과 70% ethanol 추출물은 거의 비슷한 항산화 효과를 나타내었으나, PF와 TBARs에서는 70% ethanol 추출물에서 더 우수한 항산화 효과를 나타내었다. Tyrosinase 저해활성은 열수추출물에서는 효과가 나타나지 않았으며, 70% ethanol 추출물에서는 30.32-40.56%의 저해활성을 나타내었다. Hyaluronidase 저해활성은 14.23-40.93%, 57.45-72.88%로 70% ethanol 추출물이 더 우수한 저해활성을 나타내었으며, $50-100{\mu}g/mL$의 저농도에서는 positive control로 사용한 PDTC보다 우수한 저해활성을 나타내었다. 색도는 긴병꽃풀 분말의 첨가량이 증가할수록 L 값과 a 값은 감소하는 경향을 나타내었고 b 값은 일정하게 나타내었다. 기계적 조직감은 경도와 탄력성에서 긴병꽃풀 분말의 첨가량이 증가할수록 감소하는 경향을 나타내었고, 응집성은 첨가량과 관계없이 일정한 값을 나타내었다. 씹힘성은 2.0%에서 가장 높은 수치를 나타내었으나 대조군과 첨가군 간의 유의적 차이는 나타나지 않았고, 긴병꽃풀 분말이 양갱의 기계적 조직감에 영향을 준다는 것을 확인할 수 있었다. 긴병꽃풀 분말 첨가 양갱의 관능적 기호도는 점도, 경도, 씹힘성, 탄력성, 맛, 향, 전반적인 기호도 항목에서 대조군을 제외하고 1.0% 이하 첨가군에서 기호도가 높게 나타내었으며 2.0% 첨가군은 모든 항목에서 가장 낮은 기호도를 나타내었다. 위 결과에 따라 긴병꽃풀 분말을 이용한 양갱 제조에는 1.0% 이하의 농도를 사용하여 제조하는 것이 가장 적합하다고 판단되었다.

Keywords

References

  1. Lee YK (1995) Identification and antimicrobial activity of cinnamon and clove extracts on food spoilage microorganisms. Sookmyung Women's University, Korea, p 1-4
  2. Lee MH, Jo DJ, Yoon SR, Lee GD (2007) Physicochemical properties of functional herb mixtures. J Korean Soc Food Sci Nutr, 36, 1571-1577 https://doi.org/10.3746/jkfn.2007.36.12.1571
  3. Choi GY, Bae JH, Han GJ (2007) The quality characteristics of sponge cake containing a functional and natural product (1. Mulberry leaf powder). J East Asian Soc Dietary Life, 17, 703-709
  4. Park MS (2008) A study on quality characteristics of Doraji (Platydodon grandiflorum) Yanggaeng by different pre-treatment methods and adding levels of Doraji. Dongguk University, Korea, p 1-3
  5. Jung HS, Lee JS, Yoon HH (2014) Quality characteristics of Yanggaeng sweetened with trehalose and textural changes during storage. Culi Sci Hos Res, 20, 113-124
  6. Choi EH, Kim DS, Choi SK, Park KB (2013) Optimization and quality characteristics of balsamic vinegar jelly with various gelling agents. Culi Sci Hos Res, 19, 151-163
  7. Lee SH, Hong EJ, Cho YJ (2015) Quality characteristics of Yanggaeng with Momordica charantia powder. Korean J Food Preserv, 22, 335-344 https://doi.org/10.11002/kjfp.2015.22.3.335
  8. Jang TS (2008) Systematics of the genus Glechoma L. and related genera (Nepetinae, Lamiaceae). Kyunghee University, Korea, p 2-4
  9. Kim OK (2004) Antidiabetic effect of Glechoma hederacea LINNAEUS in streptozotocin-induced diabietic rats. Korean J Pharmacogn, 35, 300-308
  10. Yao CZ, Li F, Liu YL, Zhang ZD, Wang JW, Cai BJ, Liu YY, Cheng JH, Zhao SL (1981) Influence of Chinese herb Lysimachia christinae Hance on immune responses in mice I. Immunosuppressive effect. Zhongguo Yi Xue Ke Xue Yuan Xue Bao, 3, 123-126
  11. Gu LZ, Zhang BS, Nan, JH (1988) Anti-inflammatory effects of two species of Lysimachia christinae Hance and Desmodium styracifolium (Osbeck) Merr. Zhong Yao Tong Bao, 13, 40-42
  12. Shoji N, Umeyama A, Takemoto T, Kobayashi M, Ohizumi Y (1984) Na+-K+-ATPase inhibitors from Lysimachia japonica. J Nat Prod, 47, 530-532 https://doi.org/10.1021/np50033a023
  13. Hirayama H, Wang Z, Nishi K, Ogawa A, Ishimatu T, Ueda S, Kubo T, Nohara T (1993) Effect of Desmodium styracifolium-triterpenoid on calcium oxalate renal stones. Br J Urol, 71, 143-147 https://doi.org/10.1111/j.1464-410X.1993.tb15906.x
  14. Kim HY, Kim SS, Lee CK, Choi JW (1996) Biological activities of Lysimachiae herba. I. Effects of the pretreatment of Lysimachiae herba on the enzyme activities in galactosamine intoxicated rats. Korean J Pharmacogn, 27, 58-64
  15. Ryoo HS, Nam JY, Jeong JH, In MK, Ro SH Yook CS (2000) Studies on chemical components of the aerial part of Glechoma hederacea var. longituba. Kyung Hee pharmaceutical science, 28, 25-32
  16. Folin Otto, Denis W (1912) On phosphotungsticphosphomolybdic compounds as color reagents. J Biol Chem, 12, 239-243
  17. Blois MS (1958) Antioxidant determination by the use of a stable free radical. Nature, 181, 1199-1200 https://doi.org/10.1038/1811199a0
  18. Fellegrin N, Ke R, Yang M, Rice-Evans C (1998) Screening of diatary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis (3-ethylenebenzothiazoline-6-sulfonic acid) radical cation decolorization assay. Methods Enzymol, 299, 379-389
  19. Andarwulan N, Shetty K (1999) Phenolic content in differentiated tissue cultures of untransformed and Agrobacterium-transformed roots of anise (Pimpinella anisum L.). J Agric Food Chem, 47, 1776-1780 https://doi.org/10.1021/jf981214r
  20. Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Methods Enzymol, 52, 302-310
  21. Hearing VJ (1987) Mammalian monophenol monooxygenase (tyrosinase): Purification, properties, and reactions catalyzed. Methods Enzymol, 142, 154-165
  22. Dorfman A, OTT ML (1948) A turbidimetric method for the assay of hyaluronidase. J Biol Chem, 172, 367-375
  23. Kim AJ, Han MR Lee SJ (2012) Antioxidative capacity and quality characteristics of Yanggaeng using fermented red ginseng for the elderly. Korean J Food Nutr, 25, 83-89 https://doi.org/10.9799/ksfan.2012.25.1.083
  24. Kim HJ, Jun BS, Kim SK, Cha JY, Cho YS (2000) Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower (Carthamus tinctorius L.). J Korean Soc Food Sci Nutr, 29, 1127-1132
  25. Jeong MK (2010) Monitoring the changes of 2,2-diphenyl-1-picrylhydrazyl (DPPH) absorbance and oxidation products in thermally oxidized linoleic acid. Seoul National University, Korea, p 5-6
  26. Kim CK, In MJ, Kim DC (2015) In vitro Antioxidant activity of ethanol extract from Boehmeria nivea L. leaves. Food Eng Prog, 19, 76-81 https://doi.org/10.13050/foodengprog.2015.19.1.76
  27. Bae JS, Kim TH (2011) Pancreatic lipase inhibitory and antioxidant activities of Zingiber officinale extracts. Korean J Food Preserv, 18, 390-396 https://doi.org/10.11002/kjfp.2011.18.3.390
  28. Wettasinghe M, Shahidi F (1997) Antioxidant activity of preformed cooked cured-meat pigment in a ${\beta}$ -carotene/linoleate model system. Food Chem, 58, 203-207 https://doi.org/10.1016/S0308-8146(96)00097-0
  29. Park HJ, Kang SA, Lee JY, Cho YJ (2012) Antioxidant activities of extracts from medicinal plants. Korean J Food Preserv, 19, 744-750 https://doi.org/10.11002/kjfp.2012.19.5.744
  30. Chung JH, Ho JS, Moon CK (1990) Direct interaction of streptozotocin with TBA (thiobarbituric acid) in lipid peroxidation analysis. Korean J Food Hyg Saf, 5, 237-242
  31. Jung MS, Lee GS, Chae HJ (2004) In vitro biological activity assay of ethanol extract of radish. J Korean Soc Appl Biol Chem, 47, 67-71
  32. Choi SY, Kim YC, Chang BS (2011) Inhibitory efficacy of black tea water extract on melanogenesis in melan-a cells and its action mechanisms. Korean J Microscopy, 41, 169-177
  33. Cabanes J, Chazarra S, Garcia-Carmona F (1994) Kojic acid, a cosmetic skin whitening agent, is a slow-binding inhibitor of catecholase activity of tyrosinase. J Pharm Pharmacol, 46, 982-985 https://doi.org/10.1111/j.2042-7158.1994.tb03253.x
  34. Yoon MY (2016) A study on peanut spouts extract as the anti-oxidant activity and the skin whitening cosmetic ingredients. Korean Soc Biotech Bioeng J, 31, 14-19
  35. Jeong SJ, Ko YS, Ahn NH, Kim YC (1998) Hyaluronidase inhibitor from Uncariae Ramulus et Uncus. Korean J Pharmcogn, 29, 169-172
  36. Cho KS (2015) Evaluation of Achyranthes japonica ethanol extraction on the inhibition effect of hyluronidase and lipoxygenase. J Life Sci, 25, 1370-1376 https://doi.org/10.5352/JLS.2015.25.12.1370

Cited by

  1. Quality Characteristics of Yanggaeng prepared with Osmanthus fragrans Powder vol.28, pp.2, 2017, https://doi.org/10.17495/easdl.2018.4.28.2.166