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Physicochemical characteristics of lactic acid fermented Seomaeyaksuk (Artemisia argyi H) Sikhye added with different addition ratio of MSG

MSG 첨가 비율을 달리한 섬애약쑥(Artemisia argyi H) 식혜의 유산균 발효에 따른 이화학적 특성

  • Shin, Jeong Yeon (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Shin, Jung Hye (Namhae Garlic Research Institute) ;
  • Kang, Min Jung (Namhae Garlic Research Institute) ;
  • Choi, Myung Hyo (Namhae Garlic Research Institute) ;
  • Park, Hee Rin (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Choi, Jine Shang (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Bae, Won Yeol (Department of Food technology, Gyeongsang National University) ;
  • Seo, Won Tak (Department of Food Science, Gyeongnam National University of Science and Technology)
  • Received : 2016.10.12
  • Accepted : 2017.03.24
  • Published : 2017.04.30

Abstract

For the production of ${\gamma}-aminobutyric$ acid (GABA) contents increased Seomaeyaksuk fermentant, 0, 0.25, 0.5 and 1.0% of monosodium galutamate (MSG) was added in Seomaeyaksuk water extract 15% contained Sikhye and inoculated with Lactobacillus brevis (KI271266). Physicochemical properties were sampling and analyzed at each 1 day during 3 days fermentation. Goes on fermentation periods, the turbidity was gradually reduced, but lightness and the yellowness was increased, redness was decreased. Soluble solid was also decreased. The reducing sugars content were also decreased during fermentation. pH was 4.65-4.83 before fermentation but it was lower 3.15-3.68 after three days fermentation. The GABA contents increased by fermentation periods and it was the highest in MSG 1.0% added sample (354.38 mg/L). Fructose, glucose and sucrose contents were 50-67% decreased at three days fermentation than before fermentation. Among the organic acids, propionic acid, oxalic acid, citric acid and fumaric acid contents were decreased and lactic acid, acetic acid and succinic acid were increased during fermentation periods. Contents of total polyphenol and DPPH radical scavenging activity were the highest in MSG 0.5% added sample. From these results, we confirmed that increasing of GABA content when the manufacturing of Seomaeyaksuk lactic acid fermentation product, is possibile by addition of MSG without affecting physicochemical characteristics.

GABA 함량이 증가된 섬애약쑥 발효물의 제조를 위하여 섬애약쑥 추출물을 15% 첨가하여 제조한 식혜에 MSG를 0, 0.25, 0.5 및 1.0% 첨가하고, Lactobacillus brevis(KI271266)를 접종하여 3일간 발효하면서 일자별로 시료를 취해 이화학적 특성을 분석하였다. 발효기간이 경과할수록 탁도는 점차 감소하였고, 명도와 황색도는 증가하였고, 적색도는 감소하는 경향이었다. 가용성고형분도 점차 낮아져 발효 3일에는 $19.70-20.80^{\circ}Brix$의 범위였다. 환원당의 함량은 MSG의 첨가량이 많을수록 더 높았는데, 발효기간이 경과할수록 감소하는 경향이었다. pH는 4.65-4.83이던 것이 발효 3일 후에는 3.15-3.68로 낮아졌다. MSG의 첨가농도가 높을수록 GABA의 함량도 높았으며, 발효기간이 경과할수록 그 함량도 증가하여 MSG 1.0% 첨가군에서는 354.38 mg/L로 가장 높은 함량이었다. Fructose, glucose 및 sucrose는 발효 전에 비해 3일간 발효 후에 50-67%가 감소하였다. 유기산 중 propionic acid, oxalic acid, citric acid 및 fumaric acid의 함량은 발효기간의 경과와 더불어 감소하였고, lactic acid, acetic acid, succinic acid의 함량은 점차 증가하였는데, lactic acid의 증가폭이 가장 컸다. 3일간 발효가 완료된 발효물 중의 총 페놀화합물의 함량 및 DPPH 라디칼 소거활성은 동일한 경향으로 MSG 0.5% 첨가군이 유의적으로 가장 높았고, 다음으로 1.0% 첨가군이었다. 이상의 결과로부터 섬애약쑥 유산균 발효물 제조시 MSG를 첨가함으로써 발효물의 이화학적 특성에는 영향을 미치지 않으면서 GABA의 함량 증가가 가능함을 확인할 수 있었다.

Keywords

References

  1. Hwang JY (2008) Optimization of the lactic acid fermentation of Maesil (Prunus mume). Korean J Food Nutr, 21, 391-396
  2. Cho KM, Ahn BY, Seo WT (2008) Lactic acid fermentation of Gamju manufactured using medicinal herb decoction. Korean J Food Sci Technol, 40, 649-655
  3. Jin HS (2001) Lactic acid fermentation of chestnut broth. Korean J Appl Microbiol Biotechnol, 29, 162-168
  4. Jo SJ, Oh SM, Jang EK, Hwang K, Lee SP (2008) Physicochemical properties of carrot juice fermented by Leuconostoc mesenteroides SM. J Korean Soc Food Sci Nutr, 37, 210-216 https://doi.org/10.3746/jkfn.2008.37.2.210
  5. Jeon JM, Choi SK, Kim YJ, Jang SJ, Cheon JW, Lee HS (2011) Antioxidant and antiaging effect of ginseng berry extract fermented by lactic acid bacteria. J Soc Cosmet Scientists Korea, 37, 75-81
  6. Ryu IH, Kwon TO (2013) Functional quality characteristics of extracts by sugar-leaching and lactic acid fermentation of mulberry leaves (Morus alba L.). J Seric Entomol Sci, 51, 164-172
  7. Park KB, Oh SH (2007) Cloning, sequencing and expression of a novel glutamate decarboxylase gene from a newly isolated lactic acid bacterium, Lactobacillus brevis OPK-3. Bioresource Technol, 98, 312-319 https://doi.org/10.1016/j.biortech.2006.01.004
  8. Nomura M, Nakajima I, Fujita Y, Kobayashi M, Kimoto H, Suzuki I, Aso H (1999) Lactococcus lactis contains only one glutamate decarboxylase gene. Microbiology, 145, 1375-1380 https://doi.org/10.1099/13500872-145-6-1375
  9. Krogsgaard-Larsen P (1989) GABA receptors. In: Receptor phamacology and function. Williams M, Glennon RA, Timmermans PMWM (editor), Marcel Dekker Inc, New York, NY, Korea, p 349-383
  10. Mody I, DeKoninck Y, Otis TS, Soltesz I (1994) Bringing the cleft at GABA synapses in the brain. Trends Neurosci, 17, 517-525 https://doi.org/10.1016/0166-2236(94)90155-4
  11. Lim SD, Kim KS (2009) Effects and utilization of GABA. Korean J Dairy Sci Technol, 27, 45-51
  12. Kono I, Himeno K (2000) Changes in $\gamma$-aminobutyric acid content during beni-koji making. Biosci Biotechnol Biochem, 64, 617-619 https://doi.org/10.1271/bbb.64.617
  13. Saikusa T, Horino T, Moki Y (1994) Accumulation of $\gamma$-aminobutyric acid (GABA) in the germ during water soaking. Biosci Biotechnol Biochem, 58, 2291-2292 https://doi.org/10.1271/bbb.58.2291
  14. Bown AW, Shelp BJ (1997) The metabolism and function of $\gamma$-aminobutyric acid. Plant Physiol, 115, 1-5 https://doi.org/10.1104/pp.115.1.1
  15. Miller LP, Walters JR, Martin DL (1977) Post-mortem changes implicate adenine nucleotides and pyridoxal-5'- phosphate in regulation of brain glutamate decarboxylase. Nature, 266, 847-848 https://doi.org/10.1038/266847a0
  16. Kang TJ, Oh SH (2007) Generation and utilization of GABA. BioWave, 9, 1-18
  17. https://www.khidi.or.kr (2007) GABA enhanced functional food material production technology. Issue Report, 3, 86-97
  18. Suk MH (2002) Explanation of function of GABA and possibility of development of new materia. Bulletin of Food Technol, 15, 81-85
  19. Tsushida T, Murai T (1987) Conversion of glutamic acid to $\gamma$-aminobutyric acid in tea reaves under anaerobic conditions. Agric Biol Chem, 51, 2865-2871
  20. Blankenhorn D, Phillips J, Slonczewski JL (1999) Acidand base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis. J Bacteriol, 181, 2209-2216
  21. Park SY, Kim KS, Lee MK, Lim SD (2013) Physiological characteristics and GABA production of Lactobacillus plantarum K255 isolated from kimchi. Korean J Food Sci An, 33, 595-602 https://doi.org/10.5851/kosfa.2013.33.5.595
  22. Gutfinger T (1981) Polyphenols in olive oils. J Am Oil Chem Soc, 58, 966-968 https://doi.org/10.1007/BF02659771
  23. Blois MS (1958) Antioxidant determination by the use of a stable free radical. Nature, 181, 1199-1200 https://doi.org/10.1038/1811199a0
  24. Kim SY, Choi EH (2002) Optimization for the lactic acid fermentation of mixed fruit and vegetable juice. Korean J Food Sci Technol, 34, 303-310
  25. Lee HS, Kwon SY, Lee SO, Lee SP (2016) Production of fermented Omija (Schizandra chinensis) beverage fortified with high content of gamma-amino butyric acid using Lactobacillus plantarum. Korean J Food Preserv, 23, 326-334 https://doi.org/10.11002/kjfp.2016.23.3.326
  26. Lee KS, Kim JN, Chung HC (2015) Study on anti-oxidative activities and beverage perferences relating to fermented lotus roots and Platycodon grandiflorum extracts with sugar through lactic acid fermentation. J East Asion Soc Dietary Life, 25, 183-192 https://doi.org/10.17495/easdl.2015.2.25.1.183
  27. Feehily C, Karatzas KAG (2013) Role of glutamate metabolism in bacterial responses towards acid and other stresses. J Appl Microbiol, 114, 11-24 https://doi.org/10.1111/j.1365-2672.2012.05434.x
  28. Kim NJ, Yoon KY (2013) Qualities and antioxidant activity of lactic acid fermented-potato Juice. J Korean Soc Food Sci Nutr, 42, 542-549 https://doi.org/10.3746/jkfn.2013.42.4.542

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