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마삭줄(Trachelospermum asiaticum var. intermedium nakai)로부터 추출한 pheonolic compounds의 생리활성

Biological activities of isolated phenolic compounds from Trachelospermum asiaticum var. intermedium nakai

  • 윤의태 (경북대학교 식품공학부) ;
  • 조주영 (경북대학교 식품공학부) ;
  • 정은영 (경북대학교 식품공학부) ;
  • 조재범 (경북대학교 식품공학부) ;
  • 이은호 (경북대학교 식품공학부) ;
  • 김병오 (경북대학교 식품공학부) ;
  • 조영제 (경북대학교 식품공학부)
  • Yun, Ui-Tea (School of Food science and Biotechnology, Kyungpook National University) ;
  • Cho, Ju-Young (School of Food science and Biotechnology, Kyungpook National University) ;
  • Jeong, Eun-Young (School of Food science and Biotechnology, Kyungpook National University) ;
  • Jo, Jae-Bum (School of Food science and Biotechnology, Kyungpook National University) ;
  • Lee, Eun-Ho (School of Food science and Biotechnology, Kyungpook National University) ;
  • Kim, Byung-Oh (School of Food science and Biotechnology, Kyungpook National University) ;
  • Cho, Young-Je (School of Food science and Biotechnology, Kyungpook National University)
  • 투고 : 2017.02.01
  • 심사 : 2017.03.03
  • 발행 : 2017.04.30

초록

본 연구에서 마삭줄(Trachelospermum asiaticum var. intermedium Nakai)을 용매별과 ethanol 농도별로 추출한 결과, 용매별 추출에서 ethanol 추출물이 20.8 mg/g으로 가장 높은 phenolic compounds 용출량을 보였고, 농도별 추출에서 70% ethanol 추출물이 38.1 mg/g으로 가장 높았다. 물 추출물은 16.8 mg/g의 phenolic compounds 용출량을 보였다. 마삭줄을 물과 ethanol을 추출용매로 사용하여 항산화 효과와 항통풍 및 항당뇨 효과를 확인하여 건강 기능성 식품으로의 가능성을 증명 하고자 하였다. DPPH radical 소거능 측정 결과, $100{\mu}g/mL$ phenolic compounds의 농도에서 물 추출물은 80.9%, 70% ethanol 추출물은 83.1%의 활성을 나타내었다. ABTS radical 소거능 측정 결과 $200{\mu}g/mL$ phenolic compounds의 농도에서 물 추출물은 95.0%, 70% ethanol 추출물은 95.8%의 소거능력을 나타내었다. PF 측정 결과, $100{\mu}g/mL$ phenolic compounds의 농도에서 물 추출물은 2.43 PF, 70% ethanol 추출물에서는 2.45 PF를 나타내었다. TBARs 측정 결과, $200{\mu}g/mL$ phenolic compounds의 농도에서 물과 70% ethanol 추출물에서 89.9%와 89.3%의 활성을 나타내었다. 통풍 억제 효과 측정 결과, $200{\mu}g/mL$ phenolic compounds의 농도에서 물 추출물은 33.3%, 70% ethanol 추출물은 50.5%의 저해 효과를 나타내었다. ${\alpha}-glucosidase$ 저해 효과 측정 결과, 물 추출물에는 저해 효과가 나타나지 않았고, 70% ethanol 추출물에서는 $200{\mu}g/mL$ phenolic compounds의 농도에서 92.6%의 저해 효과를 나타내었다. 이러한 결과들로 미루어 보았을 때 마삭줄 추출물은 항산화 작용과 건강 기능성 식품으로 활용 가능성을 확인할 수 있었다.

This study provided the evidence activity for biological of phenolic compounds from Trachelospermum asiaticum var. intermedium nakaier as a beauty food. The contents of phenolic compounds in water and 70% ethanol extracts were 16.8 mg/g and 38.1 mg/g, respectively. DPPH free radical scavenging activities of water and ethanol extracts at $100{\mu}g/mL$ of phenolic concentration were 80.9% and 83.1%, respectively. ABTS radical decolorization activity was 95.0% in water extracts and 95.8% in ethanol extracts at $200{\mu}g/mL$ phenolic concentration. Antioxidant protection factor (PF) was determined to 2.43 and 2.45 PF in water extracts and ethanol extracts at $100{\mu}g/mL$ phenolic concentration. TBARs of water and ethanol extracts were 89.9% and 89.3% each at $200{\mu}g/mL$ phenolic concentration. The inhibitory effect of ethanol extracts at $200{\mu}g/mL$ phenolic concentration on xanthine oxidase was 50.5%. The inhibitory activity of a-glucosidase was 92.6% in ethanol extracts at $200{\mu}g/mL$ phenolic concentration. As a result, this study will provide valuable information as a functional material with antioxidant activity, inhibitory activities of xanthin oxidase and ${\alpha}-glucosidase$.

키워드

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