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미산성 차아염소산수의 신선 채소류에 대한 살균 유효성

Disinfection efficacy of slightly acidic electrolyzed water (SlAEW) against some fresh vegetables

  • Park, Kee-Jai (Food Storage and Distribution Research Group, Korea Food Research Institute) ;
  • Lim, Jeong-Ho (Food Storage and Distribution Research Group, Korea Food Research Institute) ;
  • Jung, Heeyong (Food Storage and Distribution Research Group, Korea Food Research Institute) ;
  • Jeong, Mooncheol (Food Storage and Distribution Research Group, Korea Food Research Institute)
  • 투고 : 2016.12.16
  • 심사 : 2017.04.10
  • 발행 : 2017.04.30

초록

신선 농산물의 비가열 살균에 사용되는 살균소독제는 처리시간과 살균소독제의 농도에 비선형적인 감균효과를 나타낸다. 따라서 실제 사용에 있어서는 적정 농도와 적정 시간에 대한 고려가 매우 중요하다. 본 연구에서는 희석염산(6%, v/v)을 원료로 생성한 미산성 차아염소산수(slightly acidic electrolzyed water, SlAEW)($20{\pm}1^{\circ}C$에서의 유효염소 30 ppm, ORP $562{\pm}23mV$, pH 6.4)로 4종의 채소류(상추, 깻잎, 치콘 및 케일)에 대한 미생물 저감 특성을 분석하여 비가열 살균공정설계에 필요한 살균효과를 평가하였다. SlAEW에 30분간 3회 침지하면서 핵심인자인 유효염소와 미생물군수 및 잔류 미생물군수와의 관계를 분석하였다. 대부분의 총균수 감소는 1차 침지 초기 10분간 이루어졌으며 4종의 채소류에 생존하는 3 log CFU/g의 총균수가 침지를 통해 감소시킬 수 있는 한계값으로 판단되었다. 또한 SlAEW에 10분간 침지함으로써 감소시킬 수 있는 균수는 평균적으로 약 2 log CFU/g이었다. 초기 10분후의 감소된 유효염소는 상추, 깻잎, 치콘 및 케일에 대해 각각 2.2 ppm, 2.0 ppm, 1.7 ppm 및 2.5 ppm이었고 감소된 유효염소량의 약 50-80%가 초기 10분내에 감소되었다.

In the present study, disinfection efficacy of slightly acidic electrolyzed water [SlAEW, 30 ppm of effective chlorine at $20{\pm}1^{\circ}C$, oxidation-reduction potential (ORP) $562{\pm}23mV$, pH 6.4] on 4 kinds of vegetables (lettuce leaf, endive leaf, perilla leaf and kale leaf) was evaluated to obtain a microbial reduction characteristics which are necessary to design a process control for non-thermal sterilization of fresh vegetables. Active chlorine, residual chlorine, microbial counts and residual microbial counts, which are the key factors in the non-thermal sterilization process were measured by dipping them in SlAEW three times for 30 minutes in order to analyze the relationship between factors. Total microbial count was decreased mostly during the first 10 minutes of washing, and the limit value that can be reduced by immersion treatment was 3 log CFU/g for the total microbial count surviving in 4 kinds of vegetables. The total number of microorganism that can be reduced by washing in SIAEW for 10 min was found to be about 2 log CFU/g on average. In addition, the active chlorine decreased in the initial 10 minutes in 2.2 ppm, 2.0 ppm, 1.7 ppm and 2.5 ppm in lettuce, perilla leaf, endive leaf and kale leaf, respectively, and about 50-80% of the chlorine was reduced in the initial 10 min appear.

키워드

참고문헌

  1. Beuchat LR (1998) Surface decontamination of fruits and vegetables eaten raw: a review. Food Safety Unit, World Health Organization, Geneva, WHO/FSF/FOS/98.2, p 3-14
  2. Hricova D, Stephan R, Zweifel C (2008) Electrolyzed water and its application in the food industry. J Food Prot, 71, 1934-1947 https://doi.org/10.4315/0362-028X-71.9.1934
  3. Huang YR, Hung YC, Hsu SY, Huang YW, Hwang DF (2008) Application of electrolyzed water in the food industry. Food Control, 19, 329-345 https://doi.org/10.1016/j.foodcont.2007.08.012
  4. EPA (1999) Alternative disinfectants and oxidants guidance manual, United States Environmental Protection Agency, EPA 815-R-99-014
  5. Len SV, Hung YC, Erickson M, Kim C (2000) Ultraviolet spectrophotometric characterization and bactericidal properties of electrolyzed oxidizing water as influenced by amperage and pH. J Food Prot, 63, 1534-1537 https://doi.org/10.4315/0362-028X-63.11.1534
  6. Subrota H, Surajit M, Minz PS, Shilpa V, Yogesh K, Singh BP, Dipika Y (2012) Electrolyzed oxidized water (EOW): Non-thermal approach for decontamination of food borne microorganisms in food industry. Food Nutr Sci, 3, 760-768 https://doi.org/10.4236/fns.2012.36102
  7. Rahman SME, Ding T, Oh DH (2010) Effectiveness of low concentration electrolyzed water to inactivate foodborne pathogens under different environmental conditions. Int J Food Microbiol, 139, 147-153 https://doi.org/10.1016/j.ijfoodmicro.2010.03.020
  8. Jianxiong H, Huiying L, Yangfang W, Haijie L (2015) Effect of slightly acidic electrolyzed water (SAEW) treatment on the microbial reduction and storage quality of fresh-cut cilantro. J Food Process Pres, 39, 559-556 https://doi.org/10.1111/jfpp.12261
  9. Suzuki K, Nakamura T, Kokubo S, Tomita M (2005) The chemical properties of slightly acidic electrolyzed water prepared with hydrochloric acid as a raw material. Bokin Bobai, 33, 63-71
  10. Quan Y, Choi KD, Chung DH, Shin IS (2010) Evaluation of bactericidal activity of weakly acidic electrolyzed water (WAEW) against Vibrio vulnificus and Vibrio parahaemolyticus. Int J Food Microbiology, 136, 255-260 https://doi.org/10.1016/j.ijfoodmicro.2009.11.005
  11. Okamoto M, Komagata Y, Okuda S, Nishimoto Y, Kamoshida M, Nakamura T, Komiyama K (2006) Microbial effect of slightly acidic electrolyzed water. Bokin Bobai, 34, 3-10
  12. Issa-Zacharia A, Kamitani Y, Morita K, Iwasaki K (2010) Sanitization potency of slightly acidic electrolyzed water against pure cultures of Escherichia coli and Staphylococcus aureus, in comparison with that of other food sanitizers. Food Control, 21, 740-745 https://doi.org/10.1016/j.foodcont.2009.11.002
  13. Koide S, Takeda JI, Shi J, Shono H, Atungulu GA (2009) Disinfection efficacy of slightly acidic electrolyzed water on fresh cabbage. Food Control, 29, 294-297
  14. Suzuki K, Nakamura T, Doi T, Kokubo S, Tomita M (2005) The disinfectant effect of slightly acidic electrolyzed water prepared with hydrochloric acid to wash vegetables. Bokin Bobai, 33, 509-522
  15. American Public Health Association and Water Environment Federation (1992) Standard Methods for the Examination of Water and Wastewater. 18th ed, Method 4500-Cl G, American Public Health Association, Washington DC, USA, p 4-46
  16. Jung SH, Hur MJ, Ju JH, Kim KA, Oh SS, Go JM, Kim YH, Im JS (2006) Microbiological evaluation of raw vegetables. J Fd Hyg Safety, 21, 250-257
  17. Adams MR, Hartley AD, Cox LJ (1989) Factors affecting the efficacy of washing procedures used in the production of prepared salads. Food Microbiol, 6, 69-77 https://doi.org/10.1016/S0740-0020(89)80039-5
  18. Pirovani ME, Guemes DR, Piagnetini AM (2001) Predictive models for available chlorine depletion and total microbial count reduction during washing of fresh-cut spinach. J Food Sci, 66, 860-864 https://doi.org/10.1111/j.1365-2621.2001.tb15187.x
  19. Carmichael I, Harper IS, Conventry MJ, Taylor PWJ, Wan J, Hickey MW (1998) Bacterial colonization and biofilm development on minimally processed vegetables. J Appl Microbiol, 85, 45S-51S https://doi.org/10.1111/j.1365-2672.1998.tb05282.x
  20. Beuchat LR (2002) Ecological factors influencing survival and growth of human pathogens on raw fruits and vegetables. Microbes Infect, 4, 413-423 https://doi.org/10.1016/S1286-4579(02)01555-1
  21. Beuchat LR (2004) Difficulties in eliminating human pathogenic microorganisms on raw fruits and vegetables. Acta hort, 642, 151-160
  22. Morris CE, Monier JM, Jacques MA (1998) A technique to quantify the population size and composition of the biofilm component in communities of bacteria in the phyllosphere. Appl Environ Microbiol, 64, 4789-4795
  23. Norwood DE, Gilmour A (2000) The growth and resistance to sodium hypochlorite of Listeria monocytogenes in a steady-state multispecies biofilm. J Appl Microbiol, 88, 512-520 https://doi.org/10.1046/j.1365-2672.2000.00990.x
  24. Morris CE, Monier J, Jacques M (1997) Methods for observing microbial biofilms directly on leaf surfaces and recovering them for isolation of culturable microorganisms. Appl Environ Microbiol, 63, 1570-1576