Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Simultaneous analysis of anthocyanins and flavonols in various flower colors of Rhododendron schlippenbachii (royal azalea)

  • Oh, Soo-Min (Department of Bio-Environmental Chemistry, Chungnam National University) ;
  • Chun, Jin-Hyuk (Department of Bio-Environmental Chemistry, Chungnam National University) ;
  • Lee, Min-Ki (National Academy of Agricultural Science, Rular Development Administration) ;
  • Kim, Jung-Bong (National Academy of Agricultural Science, Rular Development Administration) ;
  • Kim, Sun-Ju (Department of Bio-Environmental Chemistry, Chungnam National University)
  • Received : 2016.05.23
  • Accepted : 2016.08.23
  • Published : 2017.03.31
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Abstract

Anthocyanins contained in Rhododendron schlippenbachii (royal azalea) are expressed in a variety of colors and affect flower colors. R. schlippenbachii flowers of seven colors (white, red group: pink, deep pink, red, purple group: light purple, purple, deep purple) were collected from the garden around KT&G building in the college of agriculture and life science. Seven types of anthocyanins [cyanidin 3-O-diglucoside, cyanidin 3-O-arabinoside-5-O-glucoside, cyanidin 3-O-galactoside, peonidin 3-O-arabinoside-5-O-glucoside, cyanidin 3-O-glucoside, cyanidin 3-O-(6"-O-malonyl) arabinoside, cyanidin 3-O-(6"-O-coumaroyl) glucoside] turned out to be from the cyanidin and peonidin series in R. schlippenbachii flowers. Also, seven types of flavonols [azaleatin 3-O-glucoside, azaleatin 3-O-arabinoside, azaleatin 3-O-rhamnoside, quercetin 3-O-galacatoside, quercetin 3-O-glucoside, quercetin 3-O-arabinoside, quercetin 3-O-rhamnoside] were identified in R. schlippenbachii flowers. Total anthocyanin amounts decreased in R. schlippenbachii flowers in the following order: 'deep pink' (8.07) > 'red' (6.37) > 'pink' (5.35) > 'deep purple' (0.78) > 'purple' (0.43) > 'light purple' ($0.22mg{\cdot}g^{-1}$ dry weight, DW) > 'white' (not detected). Total flavonol amounts decreased in the following order: 'pink' (97.78) > 'deep pink' (63.79) > 'deep purple' (61.98) > 'white' (57.58) > 'light purple' (47.06) > 'purple' (46.76) > 'red' ($7.60mg{\cdot}g^{-1}$ dry weight, DW). This study provided the quantitative and qualitative information for the variation of anthocyanin and flavonol compounds in R. schlippenbachii flowers. Furthermore, this information can contribute to the identification of anthocyanin and flavonol compounds in other Rhododendron flowers.

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References

  1. Andersen OM, Jordheim M. 2006. The anthocyanins. In Flavonoids (Chemistry, biochemistry and applications). pp. 471-515. CRC Press. Boca Raton, USA.
  2. Asen S, Budin PS. 1966. Cyanidin 3-arabinoside-5-glucoside, an anthocyanin with a new glycosidic pattern, from flowers of "Red Wing" azaleas. Phytochemistry 5:1257-1261. https://doi.org/10.1016/S0031-9422(00)86121-X
  3. Bate-Smith EC. 1953. Colour reactions of flowers attributed to (a) flavonols and (b) carotenoid oxides. Journal of Experimental Botany 4:1-9. https://doi.org/10.1093/jxb/4.1.1
  4. Castaneda OA, Pacheco HML, Paez HME, Rodriguez JA, Galn VCA. 2009. Chemical studies of anthocyanins: A review. Food Chemistry113:859-871. https://doi.org/10.1016/j.foodchem.2008.09.001
  5. Choi SJ. 2010. The Difference of anthocyanin pigment composition and color expression in fruit skin of several grape cultivars. Korean Journal of Food Preservation 17:847-852. [in Korean]
  6. Cooman LD, Everaert ESW, Faché P, Casteele VK, Sumere CFV. 1993. Flavonoid biosynthesis in petals of Rhododendron simsii. Phytochemistry 33:1419-1426. https://doi.org/10.1016/0031-9422(93)85102-W
  7. Crozier A, Burns J, Aziz AA, Stewart AJ, Rabiasz, HS, Jenkins GI, Edwards CA, Lean MEJ. 2000. Antioxidant flavonols from fruits, vegetables and beverages: Measurements and bioavailability. Biological Research 33:79-88.
  8. Delgado VF, Paredes LO.2003. Anthocyanins and betalains. In Natural colorants for food and nutraceutical uses. pp. 167-219. CRC Press. Boca Raton, USA.
  9. Griesbach RJ. 1987. Rhododendron flower color: Genetic/cultural interaction. Ph. D. dissertation, USDA, Florists & Nursery Crops Laboratory, Beltsville, Maryland, USA.
  10. Han BH. 2006. Anti-oxidative and nitric oxide production inhibitory activities of phenolic compounds from roots of Rhododendron mucronulatum. Ph.D. dissertation, Chungang Pharmacy Univ., Seoul, Korea. [in Korean]
  11. Hang NTT, Miyajima I, Ureshino K, Kobayshi N, Kurashige Y, Matusi T, Okubo H. 2011. Anthocyanins of wild Rhododendron simsii Planch. Flowers in Vietnam and Japan. Journal of the Japanese Society for Horticultural Science 80:206-213. https://doi.org/10.2503/jjshs1.80.206
  12. Hong YH, Song MJ, Han JT, Jang TO, Lee YH, Kim SH, Kim SA, Park MH, Back NI. 2003. Isolation and identification of terpenoids from the flower of Rhododendron yedoense var. poukhanense. Journal of the Korean Society of Agricultural Chemistry and Biotechnology 46:144-149. [in Korean]
  13. Kim GB, Shin KS, Kim CM, Kwon KS. 2006. Flavonoids from the leaves of Rhododendron schlippenbachii. Korean Journal of Pharmacognosy 37:177-183.
  14. Kim SI. 2006. Rhododendron (naturalized species). pp. 4-11. Landscaping tree association, Korea.
  15. Kong JM, Chia LS, Goh NK, Chia TF, Brouillard R. 2003. Analysis and biological activities of anthocyanins. Phytochemistry 64:923-933. https://doi.org/10.1016/S0031-9422(03)00438-2
  16. Li CH, Wang LS, Shu QY, Xu YJ, Zhang, J. 2008. Pigments composition of petals and floral color change during the blooming period in Rhododendron mucronulatum. Acta Horticulturae Sinca 35:1023-1030. [in Chinese]
  17. Loose RD. 1969. The flower pigments of the belgian hybrids of Rhododendron simsii and other species and varieties from Rhododendron subseries obtusum. Phytochemistry 8:253-259. https://doi.org/10.1016/S0031-9422(00)85822-7
  18. Loose RD 1970. Flavonoid glycosides in the petals of some Rhododendron species and hybrids. Phytochemistry 9:875-879. https://doi.org/10.1016/S0031-9422(00)85196-1
  19. Mizuta D, Ban T, Miyajima I, Nakatsuka A, Kobayashi, N. 2009. Comparison of flower color with anthocyanin composition patterns in evergreen azalea. Scientia Horticulturae 122:594-602. https://doi.org/10.1016/j.scienta.2009.06.027
  20. Mizuta D, Nakatsuka A, Ban T, Miyajima I, Kobayashi N. 2014. Pigment composition patterns and expression of anthocyanin biosynthesis genes in Rhododendron kiusianum, R. kaempferi, and their natural hybrids on kirishima mountain mass, Japan. Journal of the Japanese Society for Horticultural Science 83:156-162. https://doi.org/10.2503/jjshs1.CH-087
  21. Natural parks of Korea. 2009. The story of flowers - The story of Rhododendron schlippenbachii flowers (Ericaceae includes in summergreen shrub). pp. 26-27. Natural parks of Korea, Korea.
  22. To KY, Wang CK. 2006. Molecular breeding of flower color. Floriculture, Ornamental and Plant Biotechnology. pp. 300-310. Global Science Books, UK.
  23. Wu LY, Luo XD, Dai LF, Cao JF, Liu LP, Hong HY, Pan WY. 2011. Extraction and primary identification of anthocyanidins in Rhododendron Flowers. Food Science 32:19-22. [in Chinese]
  24. Wu X, Prior RL. 2005a. Systematic identification and characterization of anthocyanins by HPLC-ESI-MS/MS in common foods in the United States: Fruits and berries. Journal of Agricultural and Food Chemistry 53:2589-2599. https://doi.org/10.1021/jf048068b
  25. Wu X, Prior RL. 2005b. Identification and characterization of anthocyanins by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry in common foods in the united states: Vegetables, nuts, and grains. Journal of Agricultural and Food Chemistry 53:3101-3113. https://doi.org/10.1021/jf0478861

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