Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Callus Induction and Increase in Anti-Inflammatory Activity by Treatment of Methyl Jasmonate in Adenium obesum

석화의 캘러스 유도 및 메틸 자스모네이트 처리에 의한 항염증 활성 증진

  • Received : 2017.01.11
  • Accepted : 2017.03.06
  • Published : 2017.04.30
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Abstract

Background: Callus cultivation has the advantage of producing a large amount of tissue of a plant in a laboratory regardless of the environment, for extracting an active substance. In the present study, callus formation was induced in the leaves of the succulent plant Adenium obesum (Forssk.) Roem & Schult. After callus cultivation, anti-inflammatory activity tests were conducted, because leaves and stems of A. obesum have been reported to possess biological activity. Methods and Results: In order to induce callus formation, various concentrations of plant growth factors, such as kinetin, naphtha-leneacetic acid (NAA), 6-benzyladenine (BA), and indole-3-acetic acid (IAA) were added to MS solid medium. The maximum callus proliferation was induced by mixed medium consisting of NAA ($2mg/{\ell}$) and BA ($1mg/{\ell}$). In addition, an elicitor was added to the medium under optimal conditions for initiating suspension culture. After suspension culturing, the activities of the callus extracts were compared and analyzed. The cytotoxicity and anti-inflammatory activity tests revealed that the anti-inflammatory activity of the callus extract and the content of phenolic compounds were elevated after treatment of the callus culture with the elicitior. Conclusions: A. obesum callus might be considered as potential source of biologically active anti-inflammatory material.

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References

  1. Ahn HY, Choe DJ, Kim BK, Lee JH and Cho YS. (2015). Bioactive materials and antioxidant properties of fermented rice-bran extract. Journal of Life Science. 25:1014-1020. https://doi.org/10.5352/JLS.2015.25.9.1014
  2. Back GY. (2001). Plant tissue culture technology. Hyangmunsa. Seoul, Korea. p.59-288.
  3. Brown DCW and Thorpe TA. (1995). Crop improvement through tissue culture. World Journal of Microbiology and Biotechnology. 11:409-415. https://doi.org/10.1007/BF00364616
  4. Chukwujekwu JC, Fennell CW and van Staden J. (2002). Optimisation of the tissue culture protocol for the endangered Aloe polyphylla. South African Journal of Botany. 68:424-429. https://doi.org/10.1016/S0254-6299(15)30368-9
  5. Dimmitt MA and Hanson C. (1991). The genus Adenium in cultivation part 1: A. obesum and A. multiflorum. Cactus and Succulent Journal. 63:223-225.
  6. Farmer EE and Ryan CA. (1990). Interplant communication: Airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves. Proceedings of the National Academy of Sciences. 87:7713-7716. https://doi.org/10.1073/pnas.87.19.7713
  7. Folin O and Denis W. (1912). On phosphotungstic-phosphomolybdic compounds as color reagents. Journal of Biological Chemistry. 12:239-243.
  8. Gong HY, Lee YC and Choi SY. (2009). Neuroprotective and anti-inflammatory effects of phenolic compounds in Panax ginseng C.A. Meyer. Journal of Ginseng Research. 33:111-114. https://doi.org/10.5142/JGR.2009.33.2.111
  9. Hoffmann JJ and Cole JR. (1977). Phytochemical investigation of Adenium obesum Forskal(Apocynaceae): Isolation and identification of cytotoxic agents. Journal of Pharmaceutical Sciences. 66:1336-1338. https://doi.org/10.1002/jps.2600660935
  10. Hoper J. (1997). Spectrophotometric in vivo determination of local mitochondrial metabolism by use of a tetrazolium salt. Physiological Measurement. 18:61-66. https://doi.org/10.1088/0967-3334/18/1/004
  11. Hossain MA, Alabri THA, Al-Musalami AHS, Akhtar S and Said S. (2014). Evaluation of in vitro antioxidant potential of different polarities stem crude extracts by different extraction methods of Adenium obesum. Journal of Coastal Life Medicine. 2:699-703. https://doi.org/10.12980/JCLM.2.201414D102
  12. Hussain J, Manan S, Ahmad R, Ahmed T and Shah MM. (2013). Biotechnilogies used in genetic transformation of Triticum aestivum: A mini overview. FUUAST Journal of Biology. 3:105-109.
  13. Kang YH. (2008). Encyclopedia of life science. Life Science. Seoul. Korea. p.110-130.
  14. Kim MU and Lee SY. (2012). Induction and cultivation of callus from Aloe vera L. Food Engineering Progress. 16:226-232.
  15. Lee SE, Lee JH, Kim JK, Kim GS, Kim YO, Soe JS, Choi JH, Lee ES, Noh HJ and Kim SY. (2011). Anti-inflammatory activity of medicinal plant extracts. Korean Journal of Medicinal Crop Science. 19:217-226. https://doi.org/10.7783/KJMCS.2011.19.4.217
  16. Lisowska K and Wysokinska H. (2000). In vitro propagation of Catalpa ovata G. Don. Plant Cell, Tissue and Organ Culture. 60:171-176. https://doi.org/10.1023/A:1006461520438
  17. Miles EA, Zoubouli P, Calder PC and Phil D. (2005). Differential anti-inflammatory effects of phenolic compounds from extra virgin olive oil identified in human whole blood cultures. Nutrition. 21:389-394. https://doi.org/10.1016/j.nut.2004.06.031
  18. Takayama S, Misawa M, Ko K and Misato T. (1977). Effect of cullural conditions on the growth of Agrostemma githago cells in suspension culture and the concomitant production of an anti-plant virus substance. Physiologia Plantarum. 41:313-320. https://doi.org/10.1111/j.1399-3054.1977.tb04889.x
  19. Talmadge KW, Keegstra K, Bauer WD and Albersheim P. (1973). The structure of plant cell walls I. The macromolecular components of the walls of suspension-cultured sycamore cells with a detailed analysis of the pectic polysaccharides. Plant Physiology. 51:158-173. https://doi.org/10.1104/pp.51.1.158
  20. Tijjani A, Sallau MS and Sunus I. (2011). Synergistic activity of methanolic extract of Adenium obesum(Apocynaceae) stem-bark and oxytetracycline against some clinical bacterial isolates. Bayero Journal of Pure and Applied Sciences. 4:79-82.
  21. Yamauchi T and Abe F. (1990). Cardiac glycosides and pregnanes from Adenium obesum(studies on the constituents of Adenium. I). Chemical and Pharmaceutical Bulletin. 38:669-672. https://doi.org/10.1248/cpb.38.669