The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Effects of Patrinia Scabiosaefolia Aqueous Extract on Cytokine and NF-κB Activation in LPS-induced RAW 264.7 Cells and Mouse

패장(敗醬) 물 추출물의 LPS로 유도된 RAW 264.7 세포와 mouse 염증모델에서 cytokine 및 NF-κB의 활성에 미치는 효과

  • Ryu, Ik-Han (Dept. of Gynecology, College of Korean Medicine, Won-Kwang University) ;
  • Cho, Hae-Joong (Dept. of Obstetircs and Gynecology, College of Medicine, Won-Kwang University) ;
  • Song, Mi-Hwa (Dept. of Gynecology, College of Korean Medicine, Won-Kwang University) ;
  • Choi, Chang-Min (Dept. of Gynecology, College of Korean Medicine, Won-Kwang University)
  • 류익한 (원광대학교 한의과대학 부인과학교실) ;
  • 조해중 (원광대학교 의과대학 산부인과학교실) ;
  • 송미화 (원광대학교 한의과대학 부인과학교실) ;
  • 최창민 (원광대학교 한의과대학 부인과학교실)
  • Received : 2017.04.19
  • Accepted : 2017.05.22
  • Published : 2017.05.26
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Abstract

Objectives: The object of this study was to identify the anti-inflammatory effects of Patrinia scabiosaefolia aqueous extract (PSE). Methods: RAW 264.7 cells were pre-treated with PSE and then incubated with or without lipopolysaccharide (LPS). Cell viability, production of nitric oxide (NO), secretion of pro-inflammatory cytokine, activation of mitogen-activated protein kinases (MAPKs) and nuclear factor-kappa B (NF-${\kappa}B$) were measured. In addition, we observed mice survival rate after LPS and their cytokine levels of serum. We also observed inflammatory and hemorrhagic change on the histological sections of the liver. Results: PSE inhibited LPS-induced NO production, interleukin (IL)-6 secretion, c-Jun NH2-terminal kinase (JNK) and NF-${\kappa}B$ activation. In addition, PSE reduced the death rate of LPS-induced mice and IL-6 production on the serum of mice. PSE inhibited inflammation and hemorrhage on liver tissue as well. Conclusions: The results suggest that PSE have anti-inflammatory effects by inhibited NF-${\kappa}B$ and JNK activation, IL-6 secretion, and NO production. So PSE may be effective treatment for the inflammatory disease.

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References

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