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HSV-1 ICP27 represses NF-κB activity by regulating Daxx sumoylation

  • Kim, Ji Ae (Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University) ;
  • Choi, Mi Sun (Department of predictive toxicology, Korea Institute of Toxicology (KIT)) ;
  • Min, Jung Sun (Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University) ;
  • Kang, Inho (Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University) ;
  • Oh, Jeongho (Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University) ;
  • Kim, Jin Chul (Division of Biological Sciences, University of California) ;
  • Ahn, Jeong Keun (Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University)
  • Received : 2017.01.18
  • Accepted : 2017.03.16
  • Published : 2017.05.31

Abstract

Herpes simplex virus type 1 ICP27 is a multifunctional protein responsible for viral replication, late gene expression, and reactivation from latency. ICP27 interacts with various cellular proteins, including Daxx. However, the role of interaction between ICP27 and Daxx is largely unknown. Since Daxx is known to repress $NF-{\kappa}B$ activity, there is a possibility that ICP27 may influence the inhibitory effect of Daxx on $NF-{\kappa}B$ activity. In this study, we tested whether ICP27 affects the $NF-{\kappa}B$ activity through its interaction with Daxx. Interestingly, ICP27 enhanced the Daxx-mediated repression of $NF-{\kappa}B$ activity. In addition, we found that sumoylation of Daxx regulates its interaction with p65. ICP27 binds to Daxx, inhibits Daxx sumoylation, and enhances p65 deacetylation induced by Daxx. Consequently, ICP27 represses the $NF-{\kappa}B$ activity, by elevating the inhibitory effect of Daxx on $NF-{\kappa}B$ activity through desumoylation of Daxx.

Acknowledgement

Supported by : Chungnam National University

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