Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Evaluation of Molecular Structural Changes in Starch Depending on Amylose Content Using Dynamic Light Scattering

동적광산란법을 이용한 아밀로즈 함량에 따른 전분 분자 구조 변화 분석

  • Moon, Ju-Hyeon (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Ma, Jin-Gyeong (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Kim, Jong-Yea (Department of Food Science and Biotechnology, Kangwon National University)
  • 문주현 (강원대학교 식품생명공학과) ;
  • 마진경 (강원대학교 식품생명공학과) ;
  • 김종예 (강원대학교 식품생명공학과)
  • Received : 2017.02.24
  • Accepted : 2017.04.19
  • Published : 2017.05.31
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Abstract

To evaluate the effects of amylose content and dissolution media on the molecular structure of maize starch, changes in the hydrodynamic diameter of starch molecules were assessed via dynamic light scattering depending on amylose content and dissolution media. As the amylose content of starch increased, the hydrodynamic diameter of starch molecules proportionally decreased from 204 to 92 nm. To alter ionic strength, hydrogen bonding, or polarity of dissolution media, various contents of NaCl (1, 2, or 3 M), urea (1, 2, or 3 M), or 1-butanol (0.1, 0.5, or 1.0%) were added to media, respectively, resulting in increased hydrodynamic diameter of starch. However, the degree of expansion was dependent on amylose content and the concentration and/or type of additive. The hydrodynamic diameter of starch molecules exhibited significant correlation with amylose content obtained by size exclusion chromatography, regardless of dissolution conditions.

본 연구에서는 동적광산란법을 이용하여 아밀로즈 함량과 용매 조건에 따른 전분 분자 구조 변화를 규명하였다. 분자량이 다른 여러 가지 덱스트란 표준물질을 이용하여 동적광산란법의 정확성을 검증하였으며, 이를 전분에 적용해본 결과 아밀로즈 함량에 따른 전분 분자의 $D_h$ 변화를 규명할 수 있었고 아밀로즈 함량이 높아질수록 전분 분자의 $D_h$ 값이 증가하였다. 또한, 용매 조건에 따른 전분 분자의 $D_h$ 변화를 동적광산란법으로 규명할 수 있었으며, NaCl의 경우 농도가 높아질수록 아밀로펙틴의 $D_h$가 증가했지만, 아밀로즈의 $D_h$는 urea에 더 큰 영향을 받는 것으로 생각된다. 1-Butanol의 경우 전분 분자의 $D_h$를 증가시켰지만 주목할 만한 경향은 관찰하지 못하였다. 또한, 전분의 아밀로즈 함량과 전분 분자의 $D_h$는 유의적인 상관관계를 보였으며, 이를 활용할 경우 전분의 아밀로즈 함량을 예측하는 데 도움이 될 수 있을 것이라고 기대한다.

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References

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