Journal of the Korean Wood Science and Technology

  • 제45권3호
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  • Pages.250-257
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  • 2017
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  • 1017-0715(pISSN)
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  • 2233-7180(eISSN)
한국목재공학회 (The Korean Society of Wood Science & Technology)
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계수나무 가수분해형 탄닌의 생리활성 평가

Evaluation of Biological Activity on The Hydrolyzable Tannins of Katsura Tree (Cercidiphyllum japonicum)

  • 민희정 (강원대학교 산림환경과학대학 산림바이오소재공학과) ;
  • 이민성 (강원대학교 산림환경과학대학 산림바이오소재공학과) ;
  • 김영균 (국민대학교 삼림과학대학 임산생명공학과) ;
  • 배영수 (강원대학교 산림환경과학대학 산림바이오소재공학과)
  • Min, Hee-Jeong (Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University) ;
  • Lee, Min-Sung (Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University) ;
  • Kim, Young-Kyoon (Department of Forest Products & Biotechnology, Kookmin University) ;
  • Bae, Young-Soo (Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University)
  • 투고 : 2017.01.06
  • 심사 : 2017.02.21
  • 발행 : 2017.05.25
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초록

계수나무 잎, 목질부 및 수피부의 EtOAc 및 물 분획으로부터 gallic acid (1), methyl gallate (2), kurigalin (3), 1,2,3,6-tetra-O-galloyl-${\beta}$-D-(+)-glucose (4), 1,2,3,4,6-penta-O-galloyl-${\beta}$-D-(+)-glucose (5), 6-m-digalloyl-1,2,3,4,6-penta-O-galloyl-${\beta}$-D-(+)-glucose (6), isocorilagin (7), macabarterin (8) 등 8종류의 가수분해형 탄닌을 단리하였으며, 각 화합물에 대한 항산화 활성 및 항염활성 시험을 실시하였다. 항산화 활성은 DPPH 라디칼 소거법을 사용하였으며, 단리된 화합물 모두 대조군으로 사용된 BHT 및 ${\alpha}$-tocopherol에 비하여 매우 우수한 항산화 활성을 나타냈다. 항염활성 시험 결과 methyl gallate, 1,2,3,6-tetra-O-galloyl-${\beta}$-D-glucose 그리고 1,2,3,4,6-penta-O-galloyl-${\beta}$-D-glucose가 NO생성을 억제하였으며, 특히 methyl gallate가 가장 높은 NO 생성 억제효과를 가지는 것으로 나타났으나 항염제로 이용될 수 있는 효능을 가지고 있지는 않았다. 이와 같은 결과를 토대로 계수나무의 가수분해형 탄닌 화합물은 항염제보다 합성 항산화제를 대체할 수 있는 천연 항산화 소재로써의 적용 가능성을 나타내었다.

Eight hydrolyzable tannin compounds, such as gallic acid (1), methyl gallate (2), kurigalin (3), 1,2,3,6-tetra-O-galloyl-${\beta}$-D-(+)-glucose (4), 1,2,3,4,6-penta-O-galloyl-${\beta}$-D-(+)-glucose (5), 6-m-digalloyl-1,2,3,4,6-penta-O-galloyl-${\beta}$-D-(+)-glucose (6), isocorilagin (7), macabarterin (8), were isolated from the EtOAc and $H_2O$ soluble fractions of Katsura tree (Cercidiphyllum japonicum) leaves, wood and bark. Then antioxidative and anti-inflammatory activity were evaluated on the each isolated compound. The antioxidative test was DPPH radical scavenging activity and all of the isolated compounds indicated much higher antioxidative values compare to the controls, BHT and ${\alpha}$-tocopherol. In the anti-inflammatory test measuring nitric oxide (NO) inhibition activity, methyl gallate, 1,2,3,6-tetra-O-galloyl-${\beta}$-D-glucose and 1,2,3,4,6-penta-O-galloyl-${\beta}$-D-glucose inhibited NO production, and especially, methyl gallate showed high inhibition activity. However, the anti-inflammatory activity of the hydrolyzable tannins did not show positive effect. Based on the above results, the hydrolyzable tannins of katsura tree may be used as one of the natural biomass sources that can substitute with the synthetic antioxidant.

키워드

참고문헌

  1. Afef, A., Ines, B., Ines, S., Kita, V., Malika, K., Pascal, G., Regine, S., Anne-Marie, M., Kamel, G., Francois, L., Marie-Genevieve, D.F., Leila, C.G. 2007. Study of antimutagenic and antioxidant activities of gallic acid and 1,2,3,4,6-pentaglloylglucose from Pistacia lentiscus confirmation by microarray expression profiling. Chemico-Biological Interactions 165: 1-13. https://doi.org/10.1016/j.cbi.2006.10.003
  2. Aviram, M. 2000. Review of human studies on oxidative damage and antioxidant protection related to cardiovascular diseases. Free Radical Research 33(suppl.): 85-97.
  3. Batista, E.F., Costa, D.M., Guilhon, G.M.S.P., Muller, A.H., Santos, L.S., Arruda, M.S.P., Arruda, A.C., Silva, M.N., Silva, J.K., Secco, R.S., Filho, A.P.S.S., Figueira, B.A. 2011. Chemical constituents and allelopathic and antioxidant activities of Alchorneopsis floribunda Mull. Arg. (Euphorbiaceae). Natural Product Research: 1-8.
  4. Blois, M.S. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  5. Kim, H.K., Kim, Y.E., Do, J.R., Lee, Y.C., Lee, B.Y. 1995. Antioxidatives activity and physiological activity of some Korean Medicinal Plants. Korean Journal of Food Science and Technology 27(1): 80-85.
  6. Ko, R.K., Kim, G.O., Hyun, C.G., Jung, D.S., Lee, N.H. 2011. Compounds with tyrosinase inhibition, elastase inhibition and DPPH radical scavenging activities from the branches of Distylium racemosum Sieb. et Zucc. Phytotherapy Research 25: 1451-1456. https://doi.org/10.1002/ptr.3439
  7. Le Blanc, D.T., Akers, H.A. 1989. Maltol and ethyl maltol : From the larch tree to successful food additive. Food Technology 43: 78-84.
  8. Lee, M.S., Min, H.J., Kim, J.K., Bae, Y.S. 2016a. A polyoxygenated ellagitannin from Cercidiphyllum japonicum bark. Journal of the Korean Wood Science Technology 44(4): 551-558. https://doi.org/10.5658/WOOD.2016.44.4.551
  9. Lee, M.S., Min, H.J., Si, C.L., Bae, Y.S. 2016b. Hydrolyzable tannins from Cercidiphyllum japonicum bark. Journal of the Korean Wood Science Technology 44(4): 559-570. https://doi.org/10.5658/WOOD.2016.44.4.559
  10. Lee, T.S., Bae, Y.S. 2015. A gallotannin from Cercidiphyllum japonicum leaves. Journal of the Korean Wood Science Technology 43(5): 558-565. https://doi.org/10.5658/WOOD.2015.43.5.558
  11. Lee, T.S., Min, H.J., Bae, Y.S. 2015. Phenolic glycosides from Cercidiphyllum japonicum leaves. Journal of the Korean Wood Science Technology 43(5): 591-599. https://doi.org/10.5658/WOOD.2015.43.5.591
  12. Lim, D.K., Choi, U., Shin, D.H. 1996. Antioxidant activity of ethanol from Korean Medicinal Plants, Korean Journal of Food Science and Technology 28(1): 83-89.
  13. Liu, X., Cui, C., Zhao, M., Wang, J., Luo, W., Yang, B., Jiang, Y. 2008. Identification of phenolics in the fruit of emblica (Phyllanthus emblica L.) and their antioxidant activities. Food Chemistry 109: 909-915. https://doi.org/10.1016/j.foodchem.2008.01.071
  14. Ngoumfo, R.M., Ngounou, G.E., Tchamadeu, C.V., Qadir, M.I., Mbazoa, C.D., Begum, A., Ngninzeko, F.N., Lontsi, D., Choudhary, M.I. 2008. Inhibitory effect of macabarterin, a polyoxygenated ellagitannin from Macaranga barteri, on human neutrophil respiratory burst activity. Journal of Natural Products 71(11): 1906-1910. https://doi.org/10.1021/np8004634
  15. Park, Y.K., Min, J.Y., Lee, J.H. 2009. The effect of methyl gallate isolated from Paeonia suffruticosa on inflammatory response in LPS-stimulated RAW 264.7cells. Korean Journal of Herbology 24(4): 181-188.
  16. Sugimoto, K., Nakagawa, K., Hayashi, S., Amakura, Y., Yoshimura, M., Yoshida, T., Yamaji, R., Nakano, Y. and Inui, H. 2009. Hydrolyzable tannins as antioxidants in the leaf extract of Eucalyptus globulus possessing tyrosinase and hyaluronidase inhibitory activities. Food Science and Technology Research 15(3): 331-336. https://doi.org/10.3136/fstr.15.331
  17. Wang, K.J., Yang, C.R., Zhang, Y.J. 2007. Phenolic antioxidants from chinese toon (fresh young leaves and shoots of Toona sinensis). Food Chemicstry 101: 365-371. https://doi.org/10.1016/j.foodchem.2006.01.044
  18. Wei, W., Li, X.Y., Zhang, H.Q., Wu, S.G. 2004. Antiinflammatory and immunopharmacolohy. 1st ed. Beijing : Renminweishengchubanshe: 10-17.
  19. Williams, G.M., Iatropoulos, M.J., Whysner, J. 1999. Safety assessment of butylated hydroxyanisole and butylated hydroxytoluene as antioxidant food additives. Food and chemical Toxicology 37: 1027-1038. https://doi.org/10.1016/S0278-6915(99)00085-X
  20. Zhang, X.Y., Yuan, X.Y., Ma, J., Yuan, L.J. 2009. Research on tissue culture and regeneration of Ceridiphyllum japonicum. Northern horticulture (9): 77-79.