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미선나무 미성숙 종자의 항산화 및 미백 활성

Antioxidative Activities and Whitening Effects of Ethyl Acetate Fractions from The Immature Seeds of Abeliophyllum distichum

  • 장태원 (중원대학교 생약자원개발학과) ;
  • 박재호 (중원대학교 생약자원개발학과)
  • Jang, Tae Won (Department of Medicinal Plant Science, Jungwon University) ;
  • Park, Jae Ho (Department of Medicinal Plant Science, Jungwon University)
  • 투고 : 2016.09.12
  • 심사 : 2017.05.16
  • 발행 : 2017.05.30

초록

미선나무 미성숙 종자는 물푸레나무과의 관목으로 전세계적으로 1속 1종의 중요한 식물자원이다. 대한민국에서는 미선나무 자생지를 보존하고 멸종위기식물로 보호하고 있다. 이러한 이유로 미선나무 미성숙 종자에 대한 연구는 미비하다. 본 연구에서 미선나무 미성숙 종자의 항산화 활성과 미백 관련 단백질인 tyrosinase, TRP-1, TRP-2, MITF의 단백질 발현 및 mRNA 수준의 발현 억제 활성을 확인하였다. 미선나무 미성숙 종자는 활성산소종에 효과가 뛰어났으며, 활성산소종은 노화, 염증, 암 등 다양한 질병을 야기시킨다. 항산화 활성은 DPPH, ABTS 라디칼 소거활성 및 환원력을 평가하였으며, 이러한 활성은 페놀류 화합물과 관계가 있는 것으로 알려져 있다. 페놀류 화합물은 천연 폴리페놀이라고 불리는 파이토케미칼로서 다양한 환경적 요인에 의한 식물 방어 기작의 일환으로 생성되는 2차 대사산물이다. 페놀류 화합물은 노화, 항암을 포함한 많은 인간의 건강에 긍정적인 영향을 준다고 알려져 있다. 미선나무 미성숙 종자는 tyrosinase, TRP-1, TRP-2 단백질 및 mRNA를 조절하였으며, 이러한 요인은 멜라닌 생합성에 중요한 역할을 한다. 또한 microphthalmia-associated transcription factor (MITF)의 단백질 및 mRNA를 억제하였다. MITF는 Tyrosinase, TRP-1, TRP-2의 발현과 전사에 연관된 인자로 알려져 있다. 미선나무 미성숙 종자의 미백활성, 페놀류 화합물, 항산화 활성 사이의 연관관계를 확인하였으며, 결론적으로 미선나무 미성숙 종자는 천연 식물 자원으로부터 얻을 수 있는 항산화제 및 피부 미백을 위한 기능성 화장품 원료로 사용될 수 있다.

Abeliophyllum distichum Nakai is deciduous shrubs of flowering plant in Oleaceae. It is important plant resources and consists of one species in the world. Also the endemic plant of A. distichum has been protected and designed endangered plant in Korea. For this reason, study on the immature seeds of A. distichum (ADS) hasn't progressed. In the present study, we evaluated the antioxidant activity and inhibitory effects on proteins and mRNA levels were related in the whitening effect in B16F10 cells. ADS was effective for reaction oxygen species (ROS). ROS causes various diseases such as aging, inflammation, cancer, and etc. Antioxidant properties were evaluated DPPH, ABTS radical scavenging activity and Reducing power. Plants were known that contained phenolic compounds were related in antioxidant activity. Phenolic compounds were phytochemicals commonly named natural polyphenols. These are secondary metabolites of plants involved in the defense against different types of stresses. In results, ADS suppressed the expression and transcription of Tyrosinase, TRP-1, TRP-2, and Microphthalmia-associated transcription factor (MITF). Tyrosinase, tyrosinase-related protein 1 (TRP-1), tyrosinase-related protein 1 (TRP-2) are known to play an important role in melanin biosynthesis. MITF regulated the expression and transcription of Tyrosinase, TRP-1, and TRP-2. In conclusion, ADS was effective in both antioxidant activities and whitening effects. Also, they were associated with the content of phenolic compounds. We suggested that ADS can be use antioxidants and skin-whitening functional cosmetics material derived from natural plant resources.

키워드

참고문헌

  1. Ahn, J. H., Jin, S. H. and Kang, H. Y. 2008. LPS induces melanogenesis through p38 MAPK activation in human melanocytes. Arch. Dermatol. Res. 300, 325-329. https://doi.org/10.1007/s00403-008-0863-0
  2. AOAC. 1995. Official Methods of Analysis. pp.8-35, 14th ed., Association of Official Analytical Chemists, Washington DC, USA.
  3. Asensi, M., Ortega, A., Mena, S., Feddi, F. and Estrela, J. M. 2011. Natural polyphenols in cancer therapy. Crit. Rev. Clin. Lab. Sci. 48, 197-216. https://doi.org/10.3109/10408363.2011.631268
  4. Bentley, N. J., Eisen, T., Goding, C. R. 1994. Melanocyte-specific expression of the human tyrosinase promoter:activation by the microphthalmia gene product and role of the initiator. Mol. Cell. Biol. 14, 7996-8006. https://doi.org/10.1128/MCB.14.12.7996
  5. Bondet, V., Brand-Williams, W. and Berset, C. 1997. Kinetics and mechanisms of antioxidant activity using the DPPH free radical method. LWT - Food Sci. Technol. 30, 609-615. https://doi.org/10.1006/fstl.1997.0240
  6. Chung, J. H., Hanft, V. N. and Kang, S. 2003. Aging and photoaging. J. Am. Acad. Dermatol. 49, 690-697. https://doi.org/10.1067/S0190-9622(03)02127-3
  7. Curto, E. V., Kwong, C., Hermersdorfer, H., Glatt, H., Santis, C., Virador, V., Hearing, V. J. and Dooley, T. P. 1999. Inhibitors of mammalian melanocyte tyrosinase: in vitro comparisons of alkyl esters of gentisic acid with other putative inhibitors. Biochem. Pharmacol. 57, 663-672. https://doi.org/10.1016/S0006-2952(98)00340-2
  8. Eberlein-Konig, B., Placzek, M. and Przybilla, B. 1998. Protective effect against sunburn of combined systemic ascorbic acid (vitamin C) and d-${\alpha}$-tocopherol (vitamin E). J. Am. Dermatol. 38, 45-48. https://doi.org/10.1016/S0190-9622(98)70537-7
  9. Fantone, J. C. and Ward, P. A. 1982. Role of oxygen-derived free radicals and metabolites in leukocyte-dependent inflammatory reactions. Am. J. Pathol. 107, 395-418.
  10. Ferreres, F., Gomes, D., Valentano, P., Goncalves, R., Pio, R., Chagas, E. A., Seabra, R. and Andrade, P. 2009. Improved loquat (Eriobotrya japonica Lindl.) cultivars: Variation of phenolics and antioxidative potential. Food Chem. 114, 1109-1127.
  11. Ferreira, I. C., Baptista, P., Vilas-Boas, M. and Barros, L. 2007. Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: Individual cap and stipe activity. Food Chem. 100, 1511-1516. https://doi.org/10.1016/j.foodchem.2005.11.043
  12. Fisher, G. J., Kang, S., Varani, J., Bata-Csorgo, Z., Wan, Y., Datta, S. and Voorhees, J. J. 2002. Mechanisms of photoaging and chronological skin aging. Arch. Dermatol. 138, 1462-1470.
  13. Harold, E. S., Darrell, E. A., Evan, I. F. and John, A. M. 2007. A review of the interaction among dietary antioxidants and reactive oxygen species. J. Nutr. Biochem. 18, 567-579. https://doi.org/10.1016/j.jnutbio.2006.10.007
  14. Hearing, V. J. and Jimenez, M. 1987. Mammalian tyrosinase -the critical regulatory control point in melanocyte pigmentation. Int. J. Biochem. 19, 1141-1147. https://doi.org/10.1016/0020-711X(87)90095-4
  15. Hearing, V. J. and Tsukamoto, K. 1991. Enzymatic control of pigmentation in mammals. FASEB J. 5, 2902-2909. https://doi.org/10.1096/fasebj.5.14.1752358
  16. Hemesath, T. J., Price, E. R., Takemoto, C., Badalian, T. and Fisher, D. E. 1998. MAP kinase links the transcription factor Microphthalmia to c-kit signalling in melanocytes. Nature 391, 298-301. https://doi.org/10.1038/34681
  17. Huang, M. T., Ho, S. T. and Lee, C. Y. 1992. Phenolic compounds in food and their effects on health(II). pp.54-71, Antioxidants and cancer prevention. Am. Chem. Soc. Washington DC. USA.
  18. Jagota, S. K. and Dani, H. M. 1982. A new colorimetric technique for the estimation of vitamin C using Folin phenol reagent. Anal. Biochem. 127, 178-182. https://doi.org/10.1016/0003-2697(82)90162-2
  19. Jang, J. Y., Kim, H. N., Kim, Y. R., Choi, Y. H., Kim, B. W., Shin H. K. and Choi, B. T. 2012. Aqueous fraction from Cuscuta japonica seed suppresses melanin synthesis through inhibition of the p38 mitogen-activated protein kinase signaling pathway in B16F10 cells. J Ethnopharmacol. 141, 338-344. https://doi.org/10.1016/j.jep.2012.02.043
  20. Jeong, H. J., Park, S. B., Kim, S. and Kim, H. K. 2007. Total polyphenol content and antioxidative activity of wild grape (Vitis coignetiae) extracts depending on ethanol concentrations. J. Kor. Soc. Food Sci. Nutr. 36, 1491-1496. https://doi.org/10.3746/jkfn.2007.36.12.1491
  21. Jimenez-Cervantes, C., Solano, F., Kobayashi, T., Urabe, K., Hearing, V. J., Lozano, J. A. and Garcia-Borron, J. C. 1994. A new enzymatic function in the melanogenic pathway. The 5,6-dihydroxyindole-2-carboxylic acid oxidase activity of tyrosinase- related protein-1 (TRP-1). J. Biol. Chem. 269, 17993-18000.
  22. Kim, B., Choi, Y. E. and Kim, H. S. 2014. Eruca sativa and its flavonoid components, quercetin and isorhamnetin, improve skin barrier function by activation of peroxisome proliferator- activated receptor (PPAR)-${\alpha}$ and suppression of inflammatory cytokines. Phytother. Res. 28, 1359-1366. https://doi.org/10.1002/ptr.5138
  23. Kim, E. C., Ahn, S. Y., Hong, E. S., Li, G. H., Kim, E. K. and Row, K. H. 2005. Extraction of whitening agents from natural plants and whitening effect. Kor. J. Ind. Eng. Chem. 16, 348-353.
  24. Lee, M. S., Yoon, H. D., Kim, J. I., Choi, J. S., Byun, D. S. and Kim, H. R. 2012. Dioxinodehydroeckol inhibits melanin synthesis through PI3K/Akt signalling pathway in ${\alpha}$-melanocyte- stimulating hormone-treated B16F10 cells. Exp. Dermatol. 21, 471-473. https://doi.org/10.1111/j.1600-0625.2012.01508.x
  25. Lee, S. Y., Hwang, E. J., Kim, G. H., Choi, Y. B., Lim, C. Y. and Kim, S. M. 2005. Antifungal and antioxidant activities of extracts from leaves and flowers of Camellia japonica L. Kor. J. Med. Crop. Sci. 13, 93-100.
  26. Lin, Y. S., Chuang, M. T., Chen, C. H., Chien, M. Y. and Hou, W. C. 2012. Nicotinic acid hydroxamate downregulated the melanin synthesis and tyrosinase activity through activating the MEK/ERK and AKT/GSK3${\beta}$ signaling pathways. J. Agric. Food. Chem. 60, 4859-4864. https://doi.org/10.1021/jf301109p
  27. Marnett, L. 2000. Oxiradicals and DNA damage. Carcinogenesis 21, 361-370. https://doi.org/10.1093/carcin/21.3.361
  28. Masaki, H., Sakaki, S., Atsumi, T. and Sakurai, H. 1995. Active oxygen scavenging activity of plant extracts. Biol. Pharm. Bull. 18, 162-166. https://doi.org/10.1248/bpb.18.162
  29. Maxwell, S. R. 1995. Prospects for the use of antioxidant therapies. Drugs 49, 345-361. https://doi.org/10.2165/00003495-199549030-00003
  30. Oyaizu, M. 1986. Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn. J. Nutr. 44, 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  31. Padayatty, S. J., Katz, A., Wang, Y., Eck, P., Kwon, O., Lee, J. H., Chen, S., Corpe, C., Dutta, A., Dutta, S. K. and Levine, M. 2003. Vitamin C as an antioxidant: evaluation of its role in disease prevention. J. Am. Coll. Nutr. 22, 18-35. https://doi.org/10.1080/07315724.2003.10719272
  32. Park, H. Y., Kosmadaki, M., Yaar, M. and Gilchrest, B. A. 2009. Cellular mechanisms regulating human melanogenesis. Cell. Mol. Life. Sci. 66, 1493-1506. https://doi.org/10.1007/s00018-009-8703-8
  33. Park, J. H. 2011. Antioxidant activities and inhibitory effect on oxidative DNA damage of extracts from Abeliophylli distichi folium. Kor. J. Herbology 26, 95-99.
  34. Park, S. N. 2003. Protective effect of isoflavone, genistein from soybean on singlet oxygen induced photohemolysis of human erythrocytes. Kor. J. Food. Sci. Technol. 35, 510-518.
  35. Que, F., Mao, L. and Pan, X. 2006. Antioxidant activities of five Chinese rice wines and the involvement of phenolic compounds. Food Res. Int. 39, 581-587. https://doi.org/10.1016/j.foodres.2005.12.001
  36. Quideau, S., Deffieux, D., Douat-Casassus, C. and Pouysegu, L. 2011. Plant polyphenols: chemical properties, biological activities, and synthesis. Angew. Chem. Int. Ed. Engl. 50, 586-621. https://doi.org/10.1002/anie.201000044
  37. Seo, J. Y., Cho, K. H., Eun, H. C. and Chung, J. H. 2001. Skin aging from phenotype to mechanism. Kor. J. Investig. Dermatol. 8, 187-194.
  38. Shosuke, K., Yusuke, H. and Shinji, O. 2001. Mechanism of guanine-specific DNA damage by oxidative stress and its role in carcinogenesis and aging. Mutat Res. 488, 65-76. https://doi.org/10.1016/S1383-5742(00)00059-4
  39. Sohn, E. S., Kim, S. W., Kang, J. S. and Lee, S. P. 2004. Technology trend and patent information analysis of cosmetic materials derived from natural products. Appl. Chem. 8, 466-469.
  40. Svobodova, A., Psotova, J. and Walterova, D. 2003. Natural phenolics in the prevention of UV-induced skin damage a review. Biomed. Pap. 147, 137-145. https://doi.org/10.5507/bp.2003.019
  41. Van den Berg, R., Haenen, G. R., Van den Berg, H. and Bast, A. 1999. Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures. Food Chem. 66, 511-517. https://doi.org/10.1016/S0308-8146(99)00089-8
  42. Yang, E. S., Hwang, J. S., Choi, H. C., Hong, R. H. and Kang, S. M. 2008. The effect of genistein on melanin synthesis and in vivo whitening. Kor. J. Soc. Microbiol. Biotechnol. 36, 72-81.