Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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The Inhibitory Effects of GHJ on Allergic Inflammatory Response in Human Mast Cells (HMC-1)

고삼, 형개, 자초 혼합물(GHJ)의 인간비만세포에서의 항염증 효과

  • Hwang, Man Ki (Seocho Inuri Korean Medicine Clinic) ;
  • Choi, Young Jin (Kyung Hee Dabok Korean Medicine Clinic) ;
  • Kim, Min Ju (Yaksu Inuri Korean Medicine Clinic) ;
  • Lee, Bina (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Jung, Hyuk Sang (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Sohn, Youngjoo (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
  • Received : 2017.04.12
  • Accepted : 2017.05.29
  • Published : 2017.05.31
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Abstract

Objectives : This study aimed to evaluate inhibitory effects of GHJ on allergic inflammatory response in human mast cells (HMC-1). Methods : To investigate the inhibitory effect of GHJ (62.5, 125, 250, 500, $1000{\mu}g/mL$), HMC-1 cells were stimulated with phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI). Enzyme-linked immunosorbent assays (ELISAs), RT-PCR and Western blot analysis were investigated using GHJ extract. Results : GHJ inhibited levels of $TNF-{\alpha}$ and IL-6 of $1000{\mu}g/mL$ concentration in ELISA and mRNA expression. GHJ had inhibitory effects in level of MAPKs, $p-I{\kappa}B-{\alpha}$ and p-NF-kB also. GHJ attenuated Compound 48/80-stimulated histamine release. In addition, GHJ inhibited PCA reaction in vivo. Conclusion : This study indicated that GHJ extract can inhibit allergic responses in HMC-1 cell.

Keywords

References

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