Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Antibacterial Activity of Graviola extract to inhibit the Staphylococcus epidermidis

Staphylococcus epidermidis를 억제하는 Graviola 추출물의 항균활성

  • Choi, Jong-Hwa (Interdisciplinary Program of Perfume and Cosmetics, Graduate School of Chonnam National University) ;
  • Ohk, Seung-Ho (Interdisciplinary Program of Perfume and Cosmetics, Graduate School of Chonnam National University)
  • 최종화 (전남대학교 대학원 향장품학 협동과정) ;
  • 옥승호 (전남대학교 대학원 향장품학 협동과정)
  • Received : 2017.03.16
  • Accepted : 2017.05.12
  • Published : 2017.05.31
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Abstract

This study was carried out to verify the antimicrobial effect of Graviola leaves against pathogenic bacteria for the purpose of developing an antibacterial material. The extraction conditions of graviola leaves were $60^{\circ}C$, $80^{\circ}C$ and $98^{\circ}C$, and graviloa leaves werewater extracted at $60^{\circ}C$, $80^{\circ}C$ and $98^{\circ}C$.The extraction yields and extraction conditions were determined. The antimicrobial activity against S. epidermidis, S. aureus and E. coli TOP10 was evaluated by agar diffusion method. The extraction yields were 3.02%, 14.73% and 20.76% at $60^{\circ}C$, $80^{\circ}C$ and $98^{\circ}C$, respectively. The antimicrobial activity against S. epidermidis, S. aureus and E. coli TOP10 was found in S. epidermidis.In the samples extracted at $98^{\circ}C$, a clear zone of 13 mm was observed at 200 mg/mL and of 20 mm at 500 mg/mL. The MIC was 100 mg/mL. The higher the extraction temperature and concentration, the better was the growth inhibition effect. As a result, the natural antimicrobial activity contained in natural materials can solve the problem of resistance to antibiotics. It is considered that antimicrobial activity against S. epidermidis in skin is highly applicable to basic cosmetics and cosmetic materials.

본 연구는 그라비올라 (Graviola; Annona muricata Linn.) 잎의 항균소재 개발 목적으로 병원성 세균에 대한 항균효과를 검증하기 위해 시행된 실험 연구이다. 그라비올라 잎의 추출 온도를 $60^{\circ}C$, $80^{\circ}C$, $98^{\circ}C$ 달리하여 열수 추출하여 수율 및 추출 조건을 찾았으며 실험 균주 S. epidermidis, S. aureus 및 E. coli TOP 10에 대한 항균 활성은 한천 배지 확산법으로 평가하였다. 추출 온도 $60^{\circ}C$, $80^{\circ}C$, $98^{\circ}C$에서 3.02%, 14.73%, 20.76% 수율을 얻었고, S. epidermidis, S. aureus 및 E. coli TOP 10에 대한 항균 활성은 S. epidermidis에서 항균 활성이 나타났으며 $98^{\circ}C$에서 추출한 그라비올라 잎 시료 중 200 mg/mL에서 13 mm, 500 mg/mL에서 20 mm 투명환이 나타났다. MIC는 100 mg/mL이다. 추출 온도, 농도가 높을수록 우수한 성장억제효과를 확인하였다. 위 결과로 천연물속에 함유되어 있는 천연 항균 성분이 피부 상재균에서 우수한 항균력은 앞으로 피부미용 기초자료와 화장품 소재로서의 활용 가능성이 높을 것으로 사료된다.

Keywords

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