Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Anti-proliferative effect of methanolic extracts from Citrus junos seeds and seed oils on HT-29 human colon cancer cells and identification of their major bioactive compounds

유자(Citrus junos)씨와 유자씨 유지의 메탄올 추출물에 의한 HT-29 대장암 세포 생장 억제 효과 및 유효 성분 분석

  • Kim, Kyungeun (Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University) ;
  • Cho, Hyunnho (Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University) ;
  • Jung, Hana (Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University) ;
  • Lee, Hee Jae (Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University) ;
  • Hwang, Keum Taek (Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University)
  • 김경은 (서울대학교 식품영양학과.생활과학연구소) ;
  • 조현노 (서울대학교 식품영양학과.생활과학연구소) ;
  • 정하나 (서울대학교 식품영양학과.생활과학연구소) ;
  • 이희재 (서울대학교 식품영양학과.생활과학연구소) ;
  • 황금택 (서울대학교 식품영양학과.생활과학연구소)
  • Received : 2017.01.19
  • Accepted : 2017.04.03
  • Published : 2017.06.30
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Abstract

The purpose of this study was to investigate the anti-proliferative effect of methanolic extracts from Citrus junos (yuja) seeds and yuja seed oils against HT-29 human colon cancer cells and to identify the key compounds responsible for this effect. Extracts from yuja seeds, yuja seed oil prepared using hexane, and cold-pressed yuja seed oil were prepared using 60% methanol (ES, EHO, and ECO, respectively). The key compounds in the extracts were determined using HPLC-MS. Among the extracts, EHO and ECO inhibited proliferation of HT-29 cells. EHO and ECO were fractionated using preparative LC and the bioactive compounds were determined. Five of the fractions showed a significant anti-proliferative effect and the main compounds in the fractions were isopimpinellin, bergapten, and ichangensin. These compounds showed anti-proliferative effects on HT-29 cells when treated individually, and ichangensin showed the highest anti-proliferative activity. These results suggest that these compounds may be responsible for the anti-cancer effect of EHO and ECO.

본 연구의 목적은 유자씨와 유자씨 유지로부터 HT-29 암세포 생장 억제효과를 확인하고 주요 원인 물질을 확인하는 것이다. 유자씨, 헥산 추출 유자씨 유지, 냉압착 유자씨 유지로부터 60% 메탄올을 이용하여 추출물(각각 ES, EHO, ECO)을 얻었다. 추출물의 성분은 HPLC-MS를 이용하여 확인하였다. ES, EHO, ECO를 HT-29 세포에 처리하여 생장 억제 효과를 확인한 결과, EHO와 ECO가 유의적인 효과가 있었다(p<0.05). 반면, ES와 리모닌, 노밀린은 24시간과 48시간 처리 후에 암세포 생장 억제 효과가 없었다(p>0.05). 유자씨 유지 추출물의 암세포 생장 억제효과에 주요 역할을 하는 성분을 탐색하기 위해 제조용 LC로 EHO와 ECO를 분획하여 이 분획물의 암세포 생장 억제 효과와 조성을 확인하였다. 분획물 중에서 EHO의 3개 분획물과 ECO의 2개 분획물이 유의적인 HT-29 세포 생장 억제 효과가 있었다(p<0.05). 이 5개 분획물의 HPLC-MS 분석 결과, 아이소핌피넬린, 버갑텐, 이찬젠신이 주요 성분일 것으로 추정되었다. 아이소핌피넬린, 버갑텐, 이찬젠신을 HT-29 세포에 처리한 결과, 이찬젠신이 유의적인 생장 억제 효과가 있었고(p<0.05), 아이소핌피넬린과 버갑텐은 약간의 생장 억제 효과가 있었으나 유의적이지는 않았다(p>0.05). 따라서 아이소핌피넬린, 버갑텐, 이찬젠신이 유자씨 유지 내의 주요 암세포 생장 억제 물질이며, 이 중에서 이찬젠신이 그 활성이 가장 높은 물질인 것으로 추정한다.

Keywords

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