Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Comparison and validation of rutin and quercetin contents according to the extraction method of tartary Buckwheat (Fagopyrum tataricum Gaertn.)

쓴메밀 종자의 추출방법에 따른 루틴 및 퀘세틴 함량 비교

  • Kim, Su Jeong (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Sohn, Hwang Bae (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Geum Hee (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Yu Young (Department of Central Area, National Institute of Crop Science, Rural Development Administration) ;
  • Hong, Su Young (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Ki Deog (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Nam, Jeong Hwan (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Chang, Dong Chil (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Suh, Jong Taek (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Koo, Bon Cheol (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Yul Ho (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration)
  • 김수정 (국립식량과학원 고령지농업연구소) ;
  • 손황배 (국립식량과학원 고령지농업연구소) ;
  • 김금희 (국립식량과학원 고령지농업연구소) ;
  • 이유영 (국립식량과학원 중부작물부) ;
  • 홍수영 (국립식량과학원 고령지농업연구소) ;
  • 김기덕 (국립식량과학원 고령지농업연구소) ;
  • 남정환 (국립식량과학원 고령지농업연구소) ;
  • 장동칠 (국립식량과학원 고령지농업연구소) ;
  • 서종택 (국립식량과학원 고령지농업연구소) ;
  • 구본철 (국립식량과학원 고령지농업연구소) ;
  • 김율호 (국립식량과학원 고령지농업연구소)
  • Received : 2017.02.15
  • Accepted : 2017.03.28
  • Published : 2017.06.30
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Abstract

The stability and accuracy of ultra-performance liquid chromatography (UPLC) used for evaluating the contents of rutin and quercetin in tartary buckwheat (Fagopyrum tataricum Gaertn.) seeds extracted by seven different extraction methods were determined. The seven extraction methods were reflux extraction (RE), ultra-sonification extraction (UE), stirrer extraction (SE), RE after UE (UE+RE), RE after SE (SE+RE), UE after SE (SE+UE), and RE with UE after SE (SE+UE+RE). Among the seven extraction methods used, RE yielded comparatively higher contents of rutin (2,277 mg/ 100 g) and quercetin (158 mg/100 g) than those yielded by other six extraction methods. The intra-day repeatability and inter-day precision of RE was 0.4-3.2% considering relative standard deviation (RSD), while accuracy was 88.8-102.4%. Therefore, RE with UPLC would be a rapid, accurate, and stable method for analyzing rutin and quercetin contents in tartary buckwheat.

본 연구는 초고성능액체크로마토그래피(Ultra Performance Liquid Chromatography, UPLC)를 이용하여 쓴메밀 종자에서 루틴 및 퀘세틴 함량을 신속 정확히 평가하기 위한 효율적인 추출방법을 구명하고자 수행하였다. 추출방법으로 환류냉각추출, 초음파추출, 상온교반추출, 초음파후 환류냉각추출, 상온교반 후 환류냉각추출, 상온교반후 초음파추출, 상온교반과 초음파 후 환류냉각추출 등 7가지 방법을 비교하였다. 루틴 함량에서 7가지 추출방법은 통계적으로 유의한 차이를 보였으나, 퀘세틴 함량에서 추출방법간 유의차가 없었다. 환류냉각추출의 루틴과 퀘세틴 함량은 각각 2,277, 158 mg/100 g으로 추출방법 중 함량이 가장 많아 추출수율이 높았으며 검출횟수도 가장 높았다. 반면, 초음파 추출의 루틴과 퀘세틴의 함량이 처리구 중 가장 낮았으며, 검출횟수도 가장 적었다. 환류냉각추출에서 일내 반복성(intra-day repeatablility)과 일간 정밀성(inter-day precision)은 상대적표준편차(relative standard deviation, RSD)가 0.4-3.2%로 안정적으로 추출되었고, 정확도는 88.8-102.4%로 국제적 회수율 권고치 기준에 부합하였다. 따라서, 쓴메밀 루틴 추출방법으로는 추출 소요시간이 1시간 이내로 짧으면서 루틴 함량이 가장 안정적으로 많이 추출되는 환류냉각추출이 가장 적합하였다.

Keywords

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