Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Molecular Characterization and Expression Analysis of Interferon Regulatory Factor 8 (IRF8) in the Black Rockfish Sebastes schlegelii

조피볼락(Sebastes schlegelii) Interferon Regulatory Factor 8 (IRF8)의 분자유전학적 특성 및 발현 분석

  • Yang, Hyerim (Department of Marine Life Sciences, Jeju National University) ;
  • Kwon, Hyukjae (Department of Marine Life Sciences, Jeju National University) ;
  • Lee, Seongdo (Department of Marine Life Sciences, Jeju National University) ;
  • Bathige, S.D.N.K (Department of Marine Life Sciences, Jeju National University) ;
  • Kim, Myoung-Jin (Department of Marine Life Sciences, Jeju National University) ;
  • Lee, Jehee (Department of Marine Life Sciences, Jeju National University)
  • 양혜림 (제주대학교 해양생명과학과) ;
  • 권혁재 (제주대학교 해양생명과학과) ;
  • 이성도 (제주대학교 해양생명과학과) ;
  • ;
  • 김명진 (제주대학교 해양생명과학과) ;
  • 이제희 (제주대학교 해양생명과학과)
  • Received : 2017.06.01
  • Accepted : 2017.06.23
  • Published : 2017.06.30
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Abstract

Interferon regulatory factor 8 (IRF8) is essential for the development of B and T cells, as well as for the activity of dendritic cells and macrophages. We performed molecular characterization of IRF8 from rock fish, Sebastes schlegelii (Ss), and investigated the spatial and temporal profile of mRNA expression after challenge with lipopolysaccharide (LPS), polyinosinic:polycytidylic acid (poly I:C), or Streptococcus iniae. The full-length cDNA sequence of SsIRF8 was 1,657 bp, containing an ORF of 1,266 bp. The gene had a predicted molecular mass of 47.7 kDa and an isoelectric point of 5.99. The amino acid sequence coded by this gene showed the highest degree of identity (90.8%) and similarity (96.2%) with IRF8 from Oplegnathus fasciatus. The SsIRF8 mRNA was expressed ubiquitously, at varying levels, with the highest level of expression observed in the spleen. To confirm the role of SsIRF8 in mediating the immune response, we measured SsIRF8 mRNA expression in the splenic tissue at different time points after injection with LPS, poly I:C, or S. iniae. The qRT-PCR results showed that SsIRF8 mRNA expression in the poly I:C-injected group was highly upregulated 6 hr after exposure (P<0.05). Expression of SsIRF8 mRNA in the S. iniae-injected group peaked at 24 hr. These results suggest that SsIRF8 might be important in regulating the strength of the rockfish immune response to immunostimulatory agents.

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References

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