Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Inhibitory Effects of the Roots of Cudrania tricuspidata Bureau on Osteoclast Differentiation

꾸지뽕나무 뿌리 추출물의 파골세포 분화 억제 효과

  • Received : 2017.06.10
  • Accepted : 2017.06.20
  • Published : 2017.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Cudrania tricuspidata Bureau (Moraceae) is a traditional oriental medicine that has been widely used as anti-oxidant, anti-inflammatory and immunomodulatory in Korea. This study was performed that the 70% ethanol extract of the roots of C. tricuspidata (CTE) suppressed receptor activator of NF-${\kappa}B$ ligand (RANKL)-induced osteoclastogenesis, actin ring formation in RAW 264.7 cell lines. CTE significantly inhibited the JNK/mitogen-activated protein kinase (MAPK) signaling pathway without affecting ERK and p38 signaling in RANKL-stimulated RAW 264.7 cells. Also, CTE inhibited RANKL-induced expression of c-Fos, an upstream activator of NFATc1. Consequently, CTE suppresses osteoclast differentiation by inhibiting RANKL induced MAPK signaling pathways and disrupts the actin rings in mature osteoclasts. Thus, CTE can be used for the development of osteoporosis treatment drug with a natural material.

Keywords

References

  1. Choi, S. R., You, D. H., Kim, J. Y., Park, C. B., Kim, D. Y. and Ryu, J. (2009) Antioxidant activity of methanol extracts from Cudrania tricuspidata Bureau according to harvesting parts and time. Korean J. Medicianl Crop. Sci. 17: 115-120.
  2. Kim, O. K., Ho, J. N., Nam, D. E., Jun, W. J., Hwang, K. T., Kang, J. E., Chae, O. S. and Lee, J. M. (2012) Hepatoprotective effect of Cudrania tricuspidata extracts against oxidative damage. J. Korean Soc. Food Sci. Nutr. 41: 7-13. https://doi.org/10.3746/jkfn.2012.41.1.007
  3. Lee, H. J., Do, J. R., Kwon, J. H. and Kim, H. Y. (2011) Physiological activities of extracts from different parts of Cudrania tricuspidata. J. Korean Soc. Food Sci. Nutr. 40: 942-948. https://doi.org/10.3746/jkfn.2011.40.7.942
  4. Kwon, J. Y., Hiep, N., Kim, D. W., Hong, S. G., Guo, Y., Hwang, B. Y., Lee, H. J., Mar, W. C. and Lee, D. H. (2016) Chemical constituents isolated from the root bark of Cudrania tricuspidata and their potential neuroprotective effects. J. Nat. Prod. 79: 1938-1951. https://doi.org/10.1021/acs.jnatprod.6b00204
  5. Geng, X. L., Yang, L. B., Zhang, C., Qin, H. and Liang, Q, D. (2015) Wogonin inhibits osteoclast differentiation by inhibiting NFATc1 translocation into the nucleus. Exp. Ther. Med. 10: 1066-1070. https://doi.org/10.3892/etm.2015.2615
  6. Goltzman, D. (2002) Discoveries, drugs and skeletal disorders. Nat. Rev. Drug. Discov. 1: 784-796. https://doi.org/10.1038/nrd916
  7. Nie, S. B., Xu, J. W., Zhang, C. H., Xu, C., Liu, M. and Yu, D. G. (2015) Salicortin inhibits osteoclast differentiation and bone resorption by down-reuglating JNK and NF-${\kappa}B$/ NFATc1 signaling pathways. Biochem. Biophys. Res. Commun. 470: 61-67.
  8. Wu, Y. B., Zheng, C. J., Qin, J. P., Sun, L. N., Han, T., Jiao, L., Zhang, Q. Y. and Wu, J. Z. (2009) Antiosteoporotic activity of anthraquinones from Morinda officinalis on osteoblasts and osteoclasts. Molecules 1: 573-583.
  9. Murakiami, H., Takahashi, N., Tanaka, S., Nakamura, I., Udagawa, N., Nakajo, S., Nakaya, K., Abe, M., Yuda, Y., Konno, F., Barbier, A. and Suda, T. (1997) Tiludronate inhibits protein tyrosine phosphatase activity in osteoclasts. Bone 20: 399-404. https://doi.org/10.1016/S8756-3282(97)00025-2
  10. Hadjdaksis, D. J. and Androulakis, I. I. (2006) Bone remodeling. Ann. N. Y. Acad. Sci. 1092: 385-396. https://doi.org/10.1196/annals.1365.035
  11. Li, C. H., Yang, Z. F., Li, Z. X., Ma, Y., Zhang, L. P. Zheng, C. B., Qiu, W. W., Wu, X. A., Wang, X., Li, H., Tang, J., Qian, M., Li, D., Wang, P., Luo, J. A. and Liu, M. G. (2011) Maslinic acid suppresses osteoclastogenesis and prevents ovariectomy-induced bone loss by regulating RANKL-mediated NF-${\kappa}B$ and MAPK signaling pathways. J. Bone Miner. Res. 26: 644-656. https://doi.org/10.1002/jbmr.242
  12. Lee, J. H., Jin, H. X., Shim, H. E., Kim, H. N., Ha, H. I. and Lee, Z. H. (2010) Epigallocatechin-3-gallate inhibits osteoclastogenesis by down-regulating c-Fos expression and suppressing the nuclear factor-${\kappa}B$ signal. E. Mol. Pharmacol. 77: 17-25. https://doi.org/10.1124/mol.109.057877
  13. Teitelbaum, S. L. (2007) Osteoclasts: What do they do how do they do it? Am. J. Pathol. 170: 427-435. https://doi.org/10.2353/ajpath.2007.060834