The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Analysis of Antioxidant Activity and Cytotoxicity against Human Cancer Cell Lines of Extract from Atractylodes rhizoma fermented with Ganoderma lucidum Mycelium

창출과 영지버섯 균사체 발효 추출물의 항산화 활성 및 인체 암세포주에 대한 세포독성 분석

  • Park, Mi Hye (Dept. of Food Science and Nutrition, Kyungpook National University) ;
  • Kim, Mee Ra (Dept. of Food Science and Nutrition, Kyungpook National University)
  • 박미혜 (경북대학교 식품영양학과) ;
  • 김미라 (경북대학교 식품영양학과)
  • Received : 2017.03.11
  • Accepted : 2017.05.16
  • Published : 2017.06.30
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Abstract

Ganoderma lucidum has been traditionally used as a medicine for treatment of bronchitis, arthritis, and high blood pressure, and it has been reported to display many biological activities including anticancer and immune activities. Since mushroom mycelium is known to have excellent biological activities together with mushroom fruiting body, studies on biological activities of mushroom mycelium have been actively conducted. Thus, the present study compared the biological activities before and after the cultivation of Ganoderma lucidum mycelium on Atractylodes rhizoma. When the radical scavenging activity was assessed by the DPPH assay, ARGL (ethanol extract of Atractylodes rhizoma mycelium fermented with Ganoderma lucidum) showed radical scavenging activity of 5.58~82.56% at concentrations of $10{\sim}500{\mu}g/assay$, while AR (ethanol extract of Atractylodes rhizoma) showed radical scavenging activity of 5.27~72.08% at the same concentrations. When measured by using the ABTS assay, ARGL showed higher radical scavenging activity than AR, which was consistent with the result obtained by the DPPH assay. In the MTT assay, the cytotoxicity of ARGL against all cell lines was higher than that of AR. In particular, the cytotoxicities of AR and ARGL against Hep3B at a concentration of $400{\mu}g/assay$ were 71.81% and 86.40%, respectively. In addition, the result obtained by the SRB assay was consistent with the result obtained by the MTT assay. According to the results mentioned above, there is a high probability that medicinal herb cultures using mycelium can be used as sources of functional foods since the cytotoxicities against cancer cells and antioxidant activities increased when the mycelium was fermented with Atractylodes rhizoma.

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References

  1. Bae MJ, Ye EJ. 2007. Effect of mycelia extracts from Lentinus edodes mushroom-cultured Lonicera japonica thunberg on anticancer and antiallergy activities. J Korean Soc Food Sci Nutr 36:424-430 https://doi.org/10.3746/jkfn.2007.36.4.424
  2. Bae WC, Kim YS, Lee JW. 2005. Bioactive substances from Ganoderma lucidum. Kor J Microbiol Biotechnol 33:75-83
  3. Benzie IFF, Strain JJ. 1996. Ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal Biochem 239:70-76 https://doi.org/10.1006/abio.1996.0292
  4. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181:1199-1200 https://doi.org/10.1038/1811199a0
  5. Carbonero ER, Ruthes AC, Freitas CS, Utrilla P, Galvez J, da Silva EV. 2012. Chemical and biological properties of a highly branched ${\beta}$-glucan from edible mushroom Pleurotus sajor-caju. Carbohydr Polym 90:814-819 https://doi.org/10.1016/j.carbpol.2012.06.005
  6. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. 1987. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47:936-942
  7. Doll R, Peto R. 1981. The causes of cancer: Quantitative estimates of avoidable risks of cancer in the United States today. J Natl Cancer Inst 66:1191-1308
  8. Heim KE, Tagliaferro AR, Bobilya DJ. 2002. Flavonoid antioxidants: Chemistry, metabolism and structure-activity relationships. J Nutr Biochem 13:572-584 https://doi.org/10.1016/S0955-2863(02)00208-5
  9. Joung EM, Kim HY, Hwang IG, Jeong JH, Yu KW, Lee JS, Jeong HS. 2009. Changes of antioxidant activities on cultured ginseng with mushroom mycelia during cultivation. J Korean Soc Food Sci Nutr 39:1346-1352
  10. Kim DB, Shin GH, Lee JS, Lee OH, Park IJ, Cho JH. 2014. Antioxidant and nitrite scavenging activities of Acanthopanax senticosus extract fermented with different mushroom mycelia. Korean J Food Sci Technol 46:205-212 https://doi.org/10.9721/KJFST.2014.46.2.205
  11. Kim H, Yoon HS, Jeong JH, Jeong HS, Hwang JH, Yu KW. 2010. Enhancement of immunostimulation by fractionation of active polysaccharide from fermented ginseng with Phellinus linteus mycelium in solid culture. Korean J Food Sci 42:223-232
  12. Kim S. 2012. The Physiochemical characteristics and whitening and antioxidant effects of colored rice cultured with the mycelium of Phellinus baumii pilst. MS Thesis, Chonnam National University. Gwangju. pp.30
  13. Kim SJ, Lim DK, Hyung SW, Kim MS, Kim MN, Lee KK, Ha YL. 2004. Inhibition of lipid autoxidation by the extract of the submerged-liquid culture of mushroom in the medium containing mulberry tree powder. J Korean Soc Food Sci Nutr 33:249-254 https://doi.org/10.3746/jkfn.2004.33.2.249
  14. Lee HD, Lee KS. 2009. ${\beta}$-Glucan and glucosamine contents in various cereals cultured with mushroom mycelia. Kor J Mycol 37:167-172 https://doi.org/10.4489/KJM.2009.37.2.167
  15. Lee JW, Bang KW. 2001. Biological activity of Phllinus spp. Food Indus Nutr 6:25-33
  16. Lee NY. 2013. Antioxidant effect and tyrosinase inhibition activity of seaweeds ethanol extracts. J Korean Soc Food Nutr 42:1893-1898 https://doi.org/10.3746/jkfn.2013.42.12.1893
  17. Li X, Lin J, Han W, Wang L, Li Q, Lin B, Bai M, Zhang L, Chen D. 2012. Antioxidant ability and mechanism of rhizoma Atractylodes macrocephala. Molecules 17:13457-13472 https://doi.org/10.3390/molecules171113457
  18. Ma Z, Wang J, Zhang L. 2008. Structure and chain conformation of beta-glucan isolated from Auricularia auricula-judae. Biopolymers 89:614-622 https://doi.org/10.1002/bip.20971
  19. Madsen HL, Nielsen BR, Bertelsen G, Skibsted LH, 1996. Screening of antioxidative activity of spices. A comparison between assays based on ESR spin trapping and electrochemical measurement of oxygen consumption. Food Chem 57:331-337 https://doi.org/10.1016/0308-8146(95)00248-0
  20. Moller JKS, Madsen HL, Altonen T, Skibsted LH. 1999. Dittany (Origanum dictamnus) as a source of water extractable antioxidants. Food Chem 64:215-219 https://doi.org/10.1016/S0308-8146(98)00143-5
  21. Moreno MI, Isla MI, Sampietro AR, Vattuone MA. 2007. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71:109-114
  22. Ohno N, Miura NN, Nakajima M, Yadomae T. 2000. Antitumor 1, 3-${\beta}$-Glucan from cultured fruit body of Sparassis crispa. Biol Pharm Bull 23:866-872 https://doi.org/10.1248/bpb.23.866
  23. Park MH, Oh KY, Lee BW. 1998. Anti-cancer activity of Letinus edodes and Pleurotus. Korean J Food Sci Technol 30:702-708
  24. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1998. Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azino-bis(3-ethylene benzothiazoline-6-sulfonic acid) radical cation decolorization assay. Method Enzymol 299:379-389
  25. Roberta R, Pellegrini N, Proteggente A, Pannala A, Yang M, Catherine RE. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  26. Sohn HY, Shin YK, Kim JS. 2010. Anti-proliferative activities of solid-state fermented medicinal herbs using Phellinus baumii against human colorectal HCT116 cell. J Life Sci 20:1268-1275 https://doi.org/10.5352/JLS.2010.20.8.1268
  27. Shon MY. 2007. Antioxidant and anticancer activities of Poria cocos and Machilus thunbergii fermented with mycelial mushrooms. Food Indus Nutr 12:51-57
  28. Wang Y, Zhang L, Li Y, Hou X, Zeng F. 2004. Correlation of structure to antitumor activities of five derivatives of a eta-glucan from Poria cocos sclerotium. Carbohydr Res 339:2567-2574 https://doi.org/10.1016/j.carres.2004.08.003
  29. Wasser SP. 2002. Medical mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl Microbiol Biotechnol 60:258-274 https://doi.org/10.1007/s00253-002-1076-7
  30. Yoshikawa K, Kokudo N, Hashimoto T, Yamamoto K, Inose T, Kimura T. 2010. Novel phthalide compounds from Sparassis crispa (Hanabiratake), Hanabiratakelide A-C, exhibiting anti-cancer related activity. Biol Pharm Bull 33:1355-1359 https://doi.org/10.1248/bpb.33.1355