Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Blending effect of Campbell Early and aronia wines fermented by the mixed culture of Pichia anomala JK04 and Saccharomyces cerevisiae Fermivin

Pichia anomala JK04와 Saccharomyces cerevisiae Fermivin 혼합발효에 의한 캠벨얼리 와인과 아로니아 와인의 블렌딩 효과

  • Jeong, Hyo-sung (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Lee, Sae-Byuk (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Yeo, Su-bin (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Kim, Da-Hye (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Choi, Jun-Su (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Kim, Dong-Hwan (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Yeo, Soo-Hwan (Fermented Food Science Division, Department of Agro-Food Resources, NIAS, RDA) ;
  • Park, Heui-Dong (School of Food Science and Biotechnology, Kyungpook National University)
  • 정효성 (경북대학교 식품공학부) ;
  • 이새벽 (경북대학교 식품공학부) ;
  • 여수빈 (경북대학교 식품공학부) ;
  • 김다혜 (경북대학교 식품공학부) ;
  • 최준수 (경북대학교 식품공학부) ;
  • 김동환 (경북대학교 식품공학부) ;
  • 여수환 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 박희동 (경북대학교 식품공학부)
  • Received : 2017.03.15
  • Accepted : 2017.05.08
  • Published : 2017.06.30
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Abstract

To improve functionality and palatability of Korean Campbell Early wine. Campbell Early and aronia were fermented by either individually or at 5:5 (v/v) mixed culture of Saccharomyces cerevisiae Fermivin and Pichia anomala JK04. Blending was carried out using those two wines with different mixing ratio. Antioxidant activity analysis and sensory evaluation of blending wines were conducted. The Campbell Early wine and aronia wine blended with 9:1 (v/v) ratio showed excellent antioxidant activity and sensory scores. Total anthocyanin compound, DPPH radical scavenging activity and total phenolic compound of blending wines were higher than those of Campbell Early wine (control). Hue and intensity values increased in the order of A, B, C and D, E, F depending on P. anomala JK04 use. Anonia wine contributed the increase in a and b values of blending wine. Although blending wines fermented by P. anomala JK04 increased small amounts of aldehyde and acid compound, ester compound, the most important factor for wine aroma was also increased sharply. Adding aronia wine fermented by single culture of P. anomala JK04 (A, D) got higher color, taste, sourness and overall preference scores than other wines in the sensory evaluation. All of blending wines showed higher flavor scores than control did. This research shows a possibility of blending and utilizing non-Saccharomyces yeast for Korean wine industry.

본 연구는 국산 캠벨얼리 와인의 주질 개선과 기능성 향상을 위해 캠벨얼리 뿐만 아니라 안토시아닌과 폴리페놀 성분이 풍부하여 항산화능이 우수하다고 알려진 아로니아를 이용하여 상업용 효모인 S. cerevisiae Fermivin과 향미증진능을 지닌 P. anomala JK04를 각각 단독 또는 5:5(v/v)의 비율로 혼합발효한 후 블렌딩하는 실험을 진행하였다. 최적 블렌딩 비율을 정하기 위하여 예비실험으로 캠벨얼리 와인에 아로니아 와인을 각각 5, 10, 20%(v/v) 첨가하여 항산화능과 관능검사를 실시하였으며, 실험 결과를 바탕으로 캠벨얼리 와인과 아로니아 와인의 블렌딩 비율을 9:1(v/v)로 설정하였다. P. anomala JK04로 단독발효한 캠벨얼리 와인은 낮은 알코올 함량으로 인해 블렌딩에서 제외하였고, 나머지는 발효에 이용된 균주에 따라 블렌딩하였다. 블렌딩와인의 기능성을 평가하기 위하여 총 안토시아닌 함량, DPPH 라디칼 소거능, 총 페놀성 화합물 함량 분석을 진행하였으며, 모든 블렌딩와인에서 대조구인 S. cerevisiae로 단독발효한 캠벨얼리보다 높은 수치를 나타내었다. 블렌딩와인의 유리당 분석 결과에서는 P. anomala JK04로 단독발효한 아로니아 와인을 첨가한 시험구(A,D)에서 glucose와 fructose 같은 잔당이 남아있는 것을 확인하였으며, 유기산 분석 결과에서는 상기 두 시험구에서 특히 malic acid 함량이 높게 측정되었다. 블렌딩와인의 외관적 품질을 검사하기 위해 색도, 휘발성 향기성분, 관능검사가 수행되었으며, P. anomala JK04를 사용 여부에 따라 A, B, C와 D, E, F 순으로 hue와 intensity 값이 증가하는 경향을 나타내었다. 또한 Hunter's color value에서는 아로니아 와인의 첨가가 와인의 a value와 b value를 증가시키는 경향을 나타내었다. 휘발성 향기성분분석에서는 P. anomala JK04의 사용이 와인에 부정적인 성분인 aldehyde와 acid 함량이 소폭 증가시키긴 하였지만, 중요한 향기성분은 ester 화합물의 함량이 큰 폭으로 증가하는 것을 확인하였다. 관능검사 결과에서는 P. anomala JK04로 단독발효한 아로니아 와인을 사용한 시험구(A,D)에서 색, 맛, 신맛, 전반적인 기호도에서 다른 시험구보다 높은 점수를 받았으며, 향기 항목에서는 모든 블렌딩와인이 대조구보다 높은 점수를 받았다. 본 연구에서는 캠벨얼리 와인에 아로니아 와인을 블렌딩하여 기능성을 증가시켰으며, S. cerevisiae Fermivin과 P. anomala JK04를 혼합발효하여 향기성분의 증가 및 관능적 품질이 향상됨을 확인하였다.

Keywords

References

  1. Kim MA (2015) Fermentation characteristics of Campbell Early wine based on the amount of Korean indigenous Hanseniaspora uvarum cells. MS Thesis, Kyungpook National University, Korea, p 1
  2. Yook C, Seo MH, Kim DH, Kim JS (2007) Quality improvement of Campbell Early wine by mixing with different fruits. Korean J Food Sci Technol, 39, 390-399
  3. Kim SH, Choi IM, Cho JG, Han JH, Hwang JH, Seo HH, Yun HK (2011) Correlation analysis between fruit quality of ‘Campbell Early' grapes and climatic factors. Korean J Agric For Meteorol, 13, 93-100 https://doi.org/10.5532/KJAFM.2011.13.2.093
  4. Lee JB (2014) Effects of a Hanseniaspora uvarum isolated from Korean grapes on the quality of wine fermented using Campbell Early grape must. MS Thesis, Kyungpook National University, Korea, p 2
  5. Kim DH, Hong YA, Park HD (2008) Co-fermentation of grape must by Issatchenkia orientalis and Saccharomyces cerevisiae reduces the malic acid content in wine. Biotechnol Lett, 30, 1633-1638 https://doi.org/10.1007/s10529-008-9726-1
  6. Chang EH, Jeong ST, Jeong SM, Lim BS, Noh JH, Park KS, Park SJ, Choi JU (2011) Deacidification effect of Campbell Early must through carbonic-maceration treatment: Isolation and properties of the bacteria associated with deacidification. Korean J Food Preserv, 18, 973-979 https://doi.org/10.11002/kjfp.2011.18.6.973
  7. Lee SB, Kim DH, Park HD (2016) Effects of protectant and rehydration conditions on the survival rate and malolactic fermentation efficiency of freeze-dried Lactobacillus plantarum JH287. Appl Microbiol Biotechnol, 100, 7853-7863 https://doi.org/10.1007/s00253-016-7509-5
  8. Hong YA, Park HD (2013) Role of non-Saccharomyces yeasts in Korean wines produced from Campbell Early grapes: Potential use of Hanseniaspora uvarum as a starter culture. Food Microbiol, 34, 207-214 https://doi.org/10.1016/j.fm.2012.12.011
  9. Kahkonen MP, Hopia AI, Heinonen M (2001) Berry phenolics and their antioxidant activity. J Agric Food Chem, 49, 4076-4082 https://doi.org/10.1021/jf010152t
  10. Hou DX (2003) Potential mechanisms of cancer chemoprevention by anthocyanins. Curr Mol Med, 3, 149-159 https://doi.org/10.2174/1566524033361555
  11. Park HJ, Chung HJ (2014) Influence of the addition of aronia powder on the quality and antioxidant activity of muffins. Korean J Food Preserv, 21, 668-675 https://doi.org/10.11002/kjfp.2014.21.5.668
  12. Song JH (2016) Quality characteristics of yogurt dressing containing aronia (black chokeberry). MS Thesis, Sejong University, Korea, p 1-3
  13. Park SH, Park JH, Noh JG, Shin HM, Lee SH, Kim YH, Eom HJ (2016) Quality characteristics and antioxidant activities of aronia jams added with apple. Korean J Food Preserv, 23, 180-187 https://doi.org/10.11002/kjfp.2016.23.2.180
  14. Rojas V, Gil JV, Pinaga F, Manzanares P (2001) Studies on acetate ester production by non-Saccharomyces wine yeasts. Int J Food Microbiol, 70, 283-289 https://doi.org/10.1016/S0168-1605(01)00552-9
  15. Kwon SJ, Choi JS, Yeo SH, Park HD (2015) Fermentation characteristics of persimmon wine by the mixed culture of Pichia anomala JK04 and Saccharomyces cerevisiae Fermivin cells. Korean J Food Preserv, 22, 768-777 https://doi.org/10.11002/kjfp.2015.22.5.768
  16. Wahyono A, Lee SB, Kang WW, Park HD (2016) Improving bread quality using co-cultures of Saccharomyces cerevisiae, Torulaspora delbrueckii JK08, and Pichia anomala JK04. Ital J Food Sci, 28, 298-313
  17. Kwon SJ (2015) Characteristics of persimmon wine fermented by the mixed culture of Pichia anomala JK04 and Saccharomyces cerevisiae Fermivin. MS Thesis, Kyungpook National University, Korea, p 18-27
  18. AOAC (2000) Official method of analysis. 17th Ed, Association of official analytical chemists, Washington DC, USA, p 210-219
  19. Ahmed H (2004) Principles and reactions of protein extraction, purification and characterization. CRC Press, London, England, p 350-352
  20. Liquors Licence Aid Center (2010) Code for analysis of alcoholic beverages. National Tax Service, Seoul, Korea, p 39, p 104-202
  21. KFDA (2010) Food code. Korean Food & Drug Administration, Seoul, Korea, 10, 3-25
  22. Giusti MM, Wrolstad RE (2001) Characterization and measurement of anthocyanins by UV-visible spectroscopy. Curr Protoc Food Analyt Chem, F1.2.1-F1.2.13.
  23. Ough CS, Amerine MA (1980) Methods for analysis of musts and wines. Wiley & Sons, New York, NY, USA, p 176-180
  24. Yoo CY (2013) Antioxidant and anti-diabetes inhibitory activity of Gossypium semen extracts. MS Thesis, Kyungpook National University, Daegu, Korea, p 6
  25. Kim DH, Lee SB, Park HD (2014) Fermentation characteristics of cider from late harvest Fuji apples by a sugar tolerant yeast, Saccharomyces cerevisiae SS89. Korean J Food Preserv, 21, 917-924 https://doi.org/10.11002/kjfp.2014.21.6.917
  26. SAS (2004) SAS/STAT 9.3 user's guide. SAS Institute Inc, Cary, NC, USA, p 313-383
  27. Domizio P, Romani C, Lencioni L, Comitini F, Gobbi M, Mannazzu I, Ciani M (2011) Outlining a future for non-Saccharomyces yeasts: Selection of putative spoilage wine strains to be used in association with Saccharomyces cerevisiae for grape juice fermentation. Int J Food Micriobiol, 147, 170-180 https://doi.org/10.1016/j.ijfoodmicro.2011.03.020
  28. Park WM, Park HG, Rhee SJ, Lee CH, Yoon KE (2002) Suitability of domestic grape, cultivar Campbell's Early, for production of red wine. Korean J Food Sci Technol, 34, 590-596
  29. Jackson RS (2008) Wine science: Principles and applications. 3th ed, Academic Press, New York, NY, USA, p 1-14
  30. Lee JA, Yoon JY (2016) The quality and antioxidant properties of cookies containing aronia powder. Culi Sci Hos Res, 22, 179-189
  31. Vas G, Gal L, Harangi J, Dobo A, Vekey K (1998) Determination of volatile aroma compounds of blaufrankisch wines extracted by solid-phase microextraction. J Chromtogr Sci, 36, 505-510 https://doi.org/10.1093/chromsci/36.10.505
  32. Mingorance-Cazorla L, Clemente-Jimenez JM, Martinez-Rodriguez S, Las Heras-Vazquez FJ, Rodriguez-Vico F (2003) Contribution of different natural yeasts to the aroma of two alcoholic beverages. World J Microbiol Biotechnol, 19, 297-304 https://doi.org/10.1023/A:1023662409828
  33. Sun SY, Che CY, Sun TF, Lv ZZ, He SX, Gu HN, Shen WJ, Chi DC, Gao Y (2013) Evaluation of sequential inoculation of Saccharomyces cerevisiae and Oenococcus oeni strains on the chemical and aromatic profiles of cherry wines. Food Chem, 138, 2233-2241 https://doi.org/10.1016/j.foodchem.2012.12.032
  34. Jeong EJ, Jeon SY, Baek JH, Cha YJ (2011) Volatile flavor compounds in commercial vinegar beverages derived from fruits. J Life Sci, 21, 292-299 https://doi.org/10.5352/JLS.2011.21.2.292
  35. Molina AM, Swiegers JH, Varela C, Pretorius IS, Agosin E (2007) Influence of wine fermentation temperature on the synthesis of yeast-derived volatile aroma compounds. Appl Microbiol Biotechnol, 77, 675-687 https://doi.org/10.1007/s00253-007-1194-3
  36. Jiang B, Zhang Z (2010) Volatile compounds of young wines from Cabernet Sauvignon, Cabernet Gernischet and Chardonnay varieties grown in the Loess Plateau region of China. Molecules, 15, 9184-9196 https://doi.org/10.3390/molecules15129184
  37. Duarte WF, Dias DR, Oliveira JM, Vilanova M, Teixeira JA, Almeida e Silvar JB, Schwan RF (2010) Raspberry (Rubus idaeus L.) wine: Yeast selection, sensory evaluation and instrumental analysis of volatile and other compounds. Food Res Int, 43, 2303-2314 https://doi.org/10.1016/j.foodres.2010.08.003
  38. Dixon J, Hewett EW (2000) Factors affecting apple aroma/flavour volatile concentration: A Review. New Zeal J Crop Hortic Sci, 28, 155-173 https://doi.org/10.1080/01140671.2000.9514136

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