Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Sinus augmentation using rhBMP-2-loaded synthetic bone substitute with simultaneous implant placement in rabbits

  • Joo, Myung-Jae (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Cha, Jae-Kook (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Lim, Hyun-Chang (Department of Periodontology, Kyung Hee University School of Dentistry) ;
  • Choi, Seong-Ho (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Jung, Ui-Won (Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry)
  • Received : 2016.12.06
  • Accepted : 2017.02.15
  • Published : 2017.04.30
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Abstract

Purpose: The aim of this study was to determine the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2)-loaded synthetic bone substitute on implants that were simultaneously placed with sinus augmentation in rabbits. Methods: In this study, a circular access window was prepared in the maxillary sinus of rabbits (n=5) for a bone graft around an implant (${\varnothing}3{\times}6mm$) that was simultaneously placed anterior to the window. Synthetic bone substitute loaded with rhBMP-2 was placed on one side of the sinus to form the experimental group, and saline-soaked synthetic bone substitute was placed on the other side of the sinus to form the control group. After 4 weeks, sections were obtained for analysis by micro-computed tomography and histology. Results: Volumetric analysis showed that the median amount of newly formed bone was significantly greater in the BMP group than in the control group ($51.6mm^3$ and $46.6mm^3$, respectively; P=0.019). In the histometric analysis, the osseointegration height was also significantly greater in the BMP group at the medial surface of the implant (5.2 mm and 4.3 mm, respectively; P=0.037). Conclusions: In conclusion, an implant simultaneously placed with sinus augmentation using rhBMP-2-loaded synthetic bone substitute can be successfully osseointegrated, even when only a limited bone height is available during the early stage of healing.

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References

  1. Jensen OT, Shulman LB, Block MS, Iacono VJ. Report of the Sinus Consensus Conference of 1996. Int J Oral Maxillofac Implants 1998;13 Suppl:11-45.
  2. Wallace SS, Froum SJ. Effect of maxillary sinus augmentation on the survival of endosseous dental implants. A systematic review. Ann Periodontol 2003;8:328-43. https://doi.org/10.1902/annals.2003.8.1.328
  3. Peleg M, Mazor Z, Garg AK. Augmentation grafting of the maxillary sinus and simultaneous implant placement in patients with 3 to 5 mm of residual alveolar bone height. Int J Oral Maxillofac Implants 1999;14:549-56.
  4. Peleg M, Mazor Z, Chaushu G, Garg AK. Sinus floor augmentation with simultaneous implant placement in the severely atrophic maxilla. J Periodontol 1998;69:1397-403. https://doi.org/10.1902/jop.1998.69.12.1397
  5. Felice P, Pistilli R, Piattelli M, Soardi E, Barausse C, Esposito M. 1-stage versus 2-stage lateral sinus lift procedures: 1-year post-loading results of a multicentre randomised controlled trial. Eur J Oral Implantol 2014;7:65-75.
  6. Zitzmann NU, Scharer P. Sinus elevation procedures in the resorbed posterior maxilla. Comparison of the crestal and lateral approaches. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85:8-17. https://doi.org/10.1016/S1079-2104(98)90391-2
  7. Jung UW, Hong JY, Lee JS, Kim CS, Cho KS, Choi SH. A hybrid technique for sinus floor elevation in the severely resorbed posterior maxilla. J Periodontal Implant Sci 2010;40:76-85. https://doi.org/10.5051/jpis.2010.40.2.76
  8. Felice P, Scarano A, Pistilli R, Checchi L, Piattelli M, Pellegrino G, et al. A comparison of two techniques to augment maxillary sinuses using the lateral window approach: rigid synthetic resorbable barriers versus anorganic bovine bone. Five-month post-loading clinical and histological results of a pilot randomised controlled clinical trial. Eur J Oral Implantol 2009;2:293-306.
  9. Choi Y, Lee JS, Kim YJ, Kim MS, Choi SH, Cho KS, et al. Recombinant human bone morphogenetic protein-2 stimulates the osteogenic potential of the Schneiderian membrane: a histometric analysis in rabbits. Tissue Eng Part A 2013;19:1994-2004. https://doi.org/10.1089/ten.tea.2012.0724
  10. Seeherman H, Wozney J, Li R. Bone morphogenetic protein delivery systems. Spine (Phila Pa 1976) 2002;27:S16-23. https://doi.org/10.1097/00007632-200208151-00005
  11. Jung IH, Lim HC, Lee EU, Lee JS, Jung UW, Choi SH. Comparative analysis of carrier systems for delivering bone morphogenetic proteins. J Periodontal Implant Sci 2015;45:136-44. https://doi.org/10.5051/jpis.2015.45.4.136
  12. Alam I, Asahina I, Ohmamiuda K, Enomoto S. Comparative study of biphasic calcium phosphate ceramics impregnated with rhBMP-2 as bone substitutes. J Biomed Mater Res 2001;54:129-38. https://doi.org/10.1002/1097-4636(200101)54:1<129::AID-JBM16>3.0.CO;2-D
  13. Brodie JC, Goldie E, Connel G, Merry J, Grant MH. Osteoblast interactions with calcium phosphate ceramics modified by coating with type I collagen. J Biomed Mater Res A 2005;73:409-21.
  14. Kim JS, Cha JK, Cho AR, Kim MS, Lee JS, Hong JY, et al. Acceleration of bone regeneration by BMP-2-loaded collagenated biphasic calcium phosphate in rabbit sinus. Clin Implant Dent Relat Res 2015;17:1103-13. https://doi.org/10.1111/cid.12223
  15. Watanabe K, Niimi A, Ueda M. Autogenous bone grafts in the rabbit maxillary sinus. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1999;88:26-32. https://doi.org/10.1016/S1079-2104(99)70189-7
  16. Wada K, Niimi A, Watanabe K, Sawai T, Ueda M. Maxillary sinus floor augmentation in rabbits: a comparative histologic-histomorphometric study between rhBMP-2 and autogenous bone. Int J Periodontics Restorative Dent 2001;21:252-63.
  17. Sun XJ, Zhang ZY, Wang SY, Gittens SA, Jiang XQ, Chou LL. Maxillary sinus floor elevation using a tissue-engineered bone complex with OsteoBone and bMSCs in rabbits. Clin Oral Implants Res 2008;19:804-13. https://doi.org/10.1111/j.1600-0501.2008.01577.x
  18. Kim YS, Kim SH, Kim KH, Jhin MJ, Kim WK, Lee YK, et al. Rabbit maxillary sinus augmentation model with simultaneous implant placement: differential responses to the graft materials. J Periodontal Implant Sci 2012;42:204-11. https://doi.org/10.5051/jpis.2012.42.6.204
  19. Brunner E, Langer F. Nonparametric analysis of ordered categorical data in designs with longitudinal observations and small sample sizes. Biom J 2000;42:663-75. https://doi.org/10.1002/1521-4036(200010)42:6<663::AID-BIMJ663>3.0.CO;2-7
  20. Wikesjo UM, Qahash M, Thomson RC, Cook AD, Rohrer MD, Wozney JM, et al. rhBMP-2 significantly enhances guided bone regeneration. Clin Oral Implants Res 2004;15:194-204. https://doi.org/10.1111/j.1600-0501.2004.00971.x
  21. Yasko AW, Lane JM, Fellinger EJ, Rosen V, Wozney JM, Wang EA. The healing of segmental bone defects, induced by recombinant human bone morphogenetic protein (rhBMP-2). A radiographic, histological, and biomechanical study in rats. J Bone Joint Surg Am 1992;74:659-70. https://doi.org/10.2106/00004623-199274050-00005
  22. Sigurdsson TJ, Fu E, Tatakis DN, Rohrer MD, Wikesjo UM. Bone morphogenetic protein-2 for peri-implant bone regeneration and osseointegration. Clin Oral Implants Res 1997;8:367-74. https://doi.org/10.1034/j.1600-0501.1997.080503.x
  23. Guillot R, Gilde F, Becquart P, Sailhan F, Lapeyrere A, Logeart-Avramoglou D, et al. The stability of BMP loaded polyelectrolyte multilayer coatings on titanium. Biomaterials 2013;34:5737-46. https://doi.org/10.1016/j.biomaterials.2013.03.067
  24. Jung UW, Unursaikhan O, Park JY, Lee JS, Otgonbold J, Choi SH. Tenting effect of the elevated sinus membrane over an implant with adjunctive use of a hydroxyapatite-powdered collagen membrane in rabbits. Clin Oral Implants Res 2015;26:663-70. https://doi.org/10.1111/clr.12362
  25. Chen D, Zhao M, Harris SE, Mi Z. Signal transduction and biological functions of bone morphogenetic proteins. Front Biosci 2004;9:349-58. https://doi.org/10.2741/1090
  26. Khoury F. Augmentation of the sinus floor with mandibular bone block and simultaneous implantation: a 6-year clinical investigation. Int J Oral Maxillofac Implants 1999;14:557-64.

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