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Protective Effects of Loquat (Eriobotrya japonica Lindl.) Leaf Extract on Ethanol-Induced Liver Damage in Rats

비파 잎 추출물이 에탄올을 투여한 흰쥐의 간 손상 지표의 개선효과

  • Lee, Hwan (Dept. of Food and Nutrition, Chosun University) ;
  • Park, Yeon Jin (Dept. of Hotel Cusine & Baking, Jeonnam Provincial College) ;
  • Lee, Jae-Joon (Dept. of Food and Nutrition, Chosun University)
  • 이환 (조선대학교 식품영양학과) ;
  • 박연진 (전남도립대학교 호텔조리제빵과) ;
  • 이재준 (조선대학교 식품영양학과)
  • Received : 2017.11.09
  • Accepted : 2017.11.17
  • Published : 2017.11.30

Abstract

This study was performed to determine the hepatoprotective effects of ethanol extract of loquat leaf (LL) on alcohol-induced liver damage in rats. Sprague-Dawley rats (n=32) were divided into the following four groups: normal group (NOR), ethanol administrated group (ET), ethanol plus LL 200 mg/kg BW/day administrated group (ET-LLL), and ethanol plus LL 400 mg/kg Bw/day administrated group (ET-LLH). Body weight gain and food intake of the ET group were significantly reduced compared to those of the ET-LLL and ET-LLH groups. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) activities elevated by ethanol administration were significantly reduced by LL administration. Serum triglyceride (TG) and total cholesterol (TC) contents and hepatic TG and TC contents of the ET group were significantly elevated compared to those of the NOR group. However, TG and TC contents in the serum and liver were significantly reduced in the ET-LLH group. Hepatic glutathione (GSH) contents of the ET-LLL and ET-LLH groups were significantly elevated, and hepatic thiobarbituric acid reactive substances (TBARS) contents were reduced compared to that of the ET group. Taken together, these results suggest that LL may have a possible protective effect on the improvement of hepatic injury by ethanol administration.

Keywords

Acknowledgement

Supported by : Chosun University

References

  1. Banno N, Akihisa T, Tokuda H, Yasukawa K, Taguchi Y, Akazawa H, Ukiya M, Kimura Y, Suzuki T, Nishino H(2005) Anti-inflammatory and antitumor promoting effect of the triterpene acid from the leaves of Eriobotrya japonica. Biol Pharm Bull 28(10), 1995-1999 https://doi.org/10.1248/bpb.28.1995
  2. Bjorntorp P(1988) The associations between obesity, adipose tissue distribution and disease. Acta Med Scand 723(1), 121-134
  3. Bjorntorp P(1990) "Portal" adipose tissue as a generator of risk factors for cardiovascular disease and diabetes. Arterioscrosis 10(4), 493-496 https://doi.org/10.1161/01.ATV.10.4.493
  4. Buege JA, Aust SD(1978) The thiobarbituric acid assay. Methods Enzymol 5(4), 306-307
  5. Biggs HG, Erikson TM, Moorehead WR(1975) A manual colorimetric assay of triglyceride in serum. Clin Chem 21(3), 437-441
  6. Cerutti PA(1985) Prooxidant states and tumor promotion. Sci 227(4685), 375-381 https://doi.org/10.1126/science.2981433
  7. Chiart A, February AW, Mancru A, Lewis B(1972) Clinical and metabolic study of alcoholic ethanol. Pharmacol 7(1), 62-64 https://doi.org/10.1159/000136273
  8. Chen J, Li WL, Wu JL, Ren BR, Zhang HQ(2008) Hypoglycemic effects of a sesquiterpene glycoside isolated from leaves of loquat(Eriobotrya japonica (Thunb.) Lindl.). Phytomed 15(1-2), 98-102 https://doi.org/10.1016/j.phymed.2006.12.014
  9. Cho SY, Oh YJ, Park JY, Lee MK, Kim MJ(2003) Effect of dandelion (Taraxacum officinale) leaf extracts on hepatic antioxidative system in rats fed high cholesterol diet. J Korean Soc Food Sci Nutr 32(3), 458-463 https://doi.org/10.3746/jkfn.2003.32.3.458
  10. Choi SY, Cho HS, Sung NJ(2006) The antioxidative and nitrite scavenging ability of solvent extracts from wild grape(Vitis coignetiea) skin. J Korean Soc Food Sci Nutr 35(8), 961-966 https://doi.org/10.3746/jkfn.2006.35.8.961
  11. De Tommasi N, De Simone F, Pizza C, Mahmood N, Moore PS, Conti C, Orsi N, Stein ML(1992) Constituents of Eriobotrya japonica. A study of their antiviral properties. J Nat Prod 55(8), 1067-1073 https://doi.org/10.1021/np50086a006
  12. Folch J, Lees M, Sloane-Stanley G(1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226(1), 497-509
  13. Friedwald W, Levy R, Fredrickson D(1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18(6), 499-502
  14. Huang Y, Li J, Wang R, Wu Q, Li YH, Yu SC, Cheng WM, Wang YY(2007) Effect of triterpene acids of Eriobotrya japonica(Thunb.) Lindl. leaf on inflammatory cytokine and mediator induction from alveolar macrophages of chronic bronchitic rats. Inflamm Res 56(2), 76-82 https://doi.org/10.1007/s00011-006-5185-0
  15. Ito H, Kobayashi E, Takamatsu Y, Li SH, Hatano T, Sakagami H, Kusama K, Satoh K, Sugita D, Shimura S, Itoh Y, Yoshida T(2000) Polyphenols from Eriobotrya japonica and their cytotoxicity against human oral tumor cell lines. Chem Pharm Bull 48(5), 687-693 https://doi.org/10.1248/cpb.48.687
  16. Ito H, Kobayashi E, Li SH, Hatano T, Sugita D, Kubo N, Shimura S, Itoh Y, Tokuda H, Nishino H, Yoshida T(2002) Antitumor activity of compounds isolated from leaves of Eriobotrya japonica. J Agric Food Chem 50(8), 2400-2403 https://doi.org/10.1021/jf011083l
  17. Jung HA, Park JC, Chung HY, Kim J, Choi JS(1999) Antioxidant flavonoids and chlorogenic acid from the leaves of Eriobotrya japonica. Arch Pharm Res 22(2), 213-218 https://doi.org/10.1007/BF02976549
  18. Kanh BH, Son HY, Lee HS, Song SW(1995) Reference values of hematology and serum chemistry in kite Sprague-Dawley rats. Korean J Lab Ani Sci 11(1), 141-145
  19. Ki HY, Song SW, Ha CS, Han SS(1993) Effects of the population density on growth and various physiological values of Sprague-Dawley rats. Korean J Lab Ani Sci 9(3), 71-82
  20. Kim E, Kim MS, Rhyu DY, Min OJ, Back HY, Kim YJ, Kim HA(2009) Hypoglycemic effect of Eriobotrya japonica(E. japonica) in db/db mice. Korean J Food Nutr 22(2) 159-165
  21. Ko BS, Jang JS, Hong SM, Kim DW, Sung SR, Park HR, Lee JE, Jeon WK, Park SM(2006) Effect of new remedies mainly comprised of Hovenia dulcis Thunb on alcohol degradation and liver protection in Sprague Dawley male rats. J Korean Soc Food Sci Nutr 35(7), 828-834 https://doi.org/10.3746/jkfn.2006.35.7.828
  22. Lee CB(1982) Korean pictorial book of plants. Seoul: Hyangmoonsa, pp684-687
  23. Lee CH, Jung YJ, Park DK, Kim CW, Han YB, Lee WC, Kim JB(1993) Effect of ascorbate and ${\alpha}$-tocopherol administration on liver function in chronically ethanoltreated rats. J Korean Soc Food Nutr 22(2), 132-137
  24. Lee EH, Chyun JH(2009) Effects of Chonggukjang intake on lipid metabolism and liver function in alcoholic fatty liver rats. J Korean Soc Food Sci Nutr 38(11), 1506-1515 https://doi.org/10.3746/jkfn.2009.38.11.1506
  25. Lee H, Kim YK, Lee JJ(2015) A comparison of nutritional components of loquat(Eriobotrya japonica Lindl.) powder in different aerial components. Korean J Community Living Sci 26(3), 541-549 https://doi.org/10.7856/kjcls.2015.26.3.541
  26. Lee MH, Son YK, Han YN(2004) Tissue factor inhibitory sesquiterpene glycoside from Eriobotrya japonica. Arch Pharm Res 27(6), 619-623 https://doi.org/10.1007/BF02980160
  27. Lieber CS(1980) Interaction of ethanol with drug, hepatotoxic agent, carcinogen and vitamins. Alcoholism 25(2-3), 157-171
  28. Lieber CS(1985) Alcohol and the liver metabolism of ethanol metabolic effects and pathogenesis of injury. Acta Med Scand Suppl 703(1), 11-55
  29. Lieber CS(1994) Alcohol and the liver. Gastroenterol 106(4), 1085-1105 https://doi.org/10.1016/0016-5085(94)90772-2
  30. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ(1951) Protein measurement with the folin phenol reagent. J Biol Chem 193(1), 265-275
  31. Mendenhall CL, Bradford RH, Furman RH(1969) Effects of ethanol on glycerolipid metabolism in rat liver. Biochim Biophys Acta 187(4), 501-509 https://doi.org/10.1016/0005-2760(69)90047-2
  32. Park EM, Ye EJ, Kim SJ, Choi HI, Bae MJ(2006) Eliminatory effect of health drink containing Hovenia dulcis Thunb extract on ethanol-induced hangover in rats. Korean J Food Cult 21(1), 71-75
  33. Pirola R, Lieber CS(1972) The energy cost of the metabolism of drugs including ethanol. Pharmacol 7(3), 185-194 https://doi.org/10.1159/000136288
  34. Reeves PG, Nielson FH, Fahey Jr GC(1993) AIN-93 purified diets for laboratory rodents: final report of the American institute of nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123(11), 1939-1951 https://doi.org/10.1093/jn/123.11.1939
  35. Rosenfeld L(1989) Lipoprotein analysis. Early methods in the diagnosis of atherosclerosis. Arch Pathol Lab Med 113(10), 1101-1110
  36. Seo KH, Kim SH(2001) A study on the analysis of oriental functional beverage and on the blood alcohol concentration of rat after drinking liquors. J Korean Food Nutr 14(3), 222-227
  37. Shin HK, Seo YJ, Kim JY, Kim CS, Noh SK(2007) Onion favorably affects serum markers of ethanol-induced fatty liver in rats. Korean J Food Preserv 14(6), 662-668
  38. Soung DY, Kim JS, Chung HY, Jung HA, Park JC, Choi JS (1999) Flavonoids and chlorogenic acid from Eriobotrya japonica scavenge peroxynitrite. Nat Prod Sci 5(2), 80-84
  39. Sugano M, Goto S, Yamada Y, Yoshida K, Hashimoto Y, Matsuo T, Kimoto M(1990) Cholesterol-lowering activity of various undigested fractions of soybean protein in rats. J Nutr 120(9), 974-978
  40. Taniguchi S, Imayoshi Y, Kobayashi E, Takamatsu Y, Ito H, Hatano T, Sakagami H, Tokuda H, Nishino H, Sugita D, Shimura S and Yoshida T(2002) Production of bioactive triterpenes by Eriobotrya japonica calli. Phytochem 59(3), 315-323 https://doi.org/10.1016/S0031-9422(01)00455-1
  41. Tietze F(1969) Enzymatic methods for quantitative determination of nanogram amounts of total and oxidized glutathione. Anal Biochem 27(3), 502-522 https://doi.org/10.1016/0003-2697(69)90064-5
  42. Tuma DJ, Casey CA(2003) Dangerous byproducts of alcohol breakdown-focus on adducts. Alcohol Res Health 27(4), 258-290
  43. Whang TE, Lim HO, Lee JW(1996) Anticancer effect of Eriobotrya japonica Lindl by specificity test with serveral cancer cell line. Korean J Medi Crop Sci 4(4), 314-320
  44. Won HR, Lee SG, Park DY(2005) Effects of hot water soluble extract from green tea on the lipid metabolism and antioxidant effect in rats fed animal or vegetable protein and a hypercholesterol diet. Korean J Community Living Sci 16(12) 39-45
  45. Yook CS(1998) Coloured medicinal plants of korea. Seoul: Academic Publishing Co, p261
  46. Zheng CX, Yim DS, Lee SY(2004) The effects of Ka-Mi-Chung-Gan-Tang on rat with alcoholic fatty liver. Korean J Pharmacogn 35(3), 229-232
  47. Zlatkis A, Zak B(1969) Study of a new cholesterol reagent. Anal Biochem 29(1), 143-148 https://doi.org/10.1016/0003-2697(69)90017-7

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