The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Evaluation of Immunopotentiation Activities of Combined Extract of Silkworm and Food material

누에 복합 추출물의 면역 활성 증진 효과

  • Lee, AhReum (College of Korean Medicine, Daegu Haany University) ;
  • Kim, SooHyun (College of Korean Medicine, Daegu Haany University) ;
  • Kim, SuJi (College of Korean Medicine, Daegu Haany University) ;
  • Kim, KyeongJo (College of Korean Medicine, Daegu Haany University) ;
  • Lee, Young-Cheol (College of Korean Medicine, Sangji University) ;
  • Roh, Seong-Soo (College of Korean Medicine, Daegu Haany University)
  • 이아름 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김수현 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김수지 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김경조 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이영철 (상지대학교 한의과대학 본초학교실) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2017.06.11
  • Accepted : 2017.07.15
  • Published : 2017.07.30
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Abstract

Objectives : Silkworm is known as immunomodulatory substances and contain various bioactive compounds such as serine, tyrosine and alanine. The aim of this study was to investigated the immunopotentiating activity of combine extract that silkworm and food materials (Eucommia ulmoides, Angelica gigas, Acanthopanax, Allium hookeri, Cinnamomum cassia, Liriope platyphylla, Curcuma longa, Achyranthes japonica, Alpinia oxyphylla, Adenophora triphylla). Methods : Among 10 kinds of food materials, to select food materials with the effect of enhancing the immune function mouse splenocyte proliferation ability was measured by 3-[4,5-dimethylthiazol-2-y]-2,5-diphenyl terazolium bromide (MTT) assay. Then, combine extract of silkworm and food materials were evaluated that mouse splenocyte proliferation ability by EZ-cytox cell viability assay. Morever, cytokines production such as IL-2, IL-4, IL10, IL12, $IFN-{\gamma}$ on mouse T lymphocyte stimulated with concanavalin A (ConA) was measured. Results : Eucommia ulmoides, Acanthopanax, Allium hookeri, Cinnamomum cassia, Liriope platyphylla has high proliferation ability of mouse splenocyte compared with Curcuma longa, Achyranthes japonica, Alpinia oxyphylla, Adenophora triphylla. The silkworm and food material combined extract has a relatively high proliferation ability of mouse splenocyte proliferation when the silkworm and food materials are used as a single material. In particularly, combined extract of silkworm and Cinnamomum cassia was stimulate cytokine production on T lymphocyte such as IL12, $IFN-{\gamma}$. Combined extract of silkworm and Liriope platyphylla was stimulate cytokine production on T lymphocyte such as IL2, IL4, IL10. Conclusion : In conclusion, the combined extract of the silkworm and Cinnamomum cassia or Liriope platyphylla may enhance immune function by regulating mouse splenocyte proliferation and stimulating cytokine production.

Keywords

References

  1. Yoon JW, Rhee SK, Lee KB. Effects of silkworm extract powder on plasma lipids and glucose in rats. Korean J. Food & Nutr. 2005 ; 18(2) : 140-5.
  2. Kwon YJ, Kim HJ. Quality characteristics of white pan bread containing heat treated silkworm steam extract. Food Service Industry Journal. 2016 ; 12(1) : 87-97. https://doi.org/10.22509/kfsa.2016.12.1.009
  3. Ryu KS, Lee HS, Kim SY. Pharmacodynamic study of silkworm powder in mice administered to maltose, sucrose and lactose. Korean J. Seric. Sci. 1999 ; 41(1) : 9-13.
  4. Chon JW, Kweon HY, Jo YY, Park MK, Son YH, Lee HS. A study on the develipment of functional cosmetics using silk-gland powder of silkworm. J. Soc. Cosmet. Scientists Korea. 2012 ; 38(2) : 163-9. https://doi.org/10.15230/SCSK.2012.38.2.163
  5. Cha JY, Kim YS, Kang PD, Ahn HY, Eom KE, Cho YS. Biological activity and chemical characteristics of fermented silkworm powder by mold. Jounal of Life Science. 2010 ; 20(2) : 237-44. https://doi.org/10.5352/JLS.2010.20.2.237
  6. Kim SH, Je YH, Yun EY, Kang SW, Kim KY, Kang SK. Isolation and characterization of inducible genes from Bombyx mori injected with E.coli by differential screening. Korean J. Seric. Sci. 1996 ; 38(1) : 19-24.
  7. Hyun JW, Lee KG, Yeo JH, Choe TB. Hair care effects of hair cosmetics including low molecular weight silk peptide component and micro structure analysis. Korean J. Biotechnol. Bioeng. 2008 ; 23(5) : 439-44.
  8. Yoo JM, Hwang JS, Goo TW, Yun EY. Comparative analysis of nutritional and harmful components in Korean and Chinese mealworms (Tenebrio molitor). J Korean Soc Food Sci Nutr. 2013 ; 42(2) : 249-54. https://doi.org/10.3746/jkfn.2013.42.2.249
  9. Ryu HS, Kim J, Kim HS. Enhancing effect of Sorghum bicolor L. Moench (Sorghum, su-su) Extracts on mouse spleen and macrophage cell activation. Korean J. Food & Nutr. 2006 ; 19(2) : 176-82.
  10. Han SB, Kim YH, Lee CW, Park SM, Lee HY, Ahn KS, Kim IH, Kim HM, Characteristic immunostimulation by angelan isolated from Angelica gigas Nakai. Immunopharmacology. 1998 ; 40(1) : 39-48. https://doi.org/10.1016/S0162-3109(98)00026-5
  11. Park HR, Yu YB, Yee ST, Jo SK, Enhancement of immune response by water extract of Angelica gigas Nakai. J. Life Resources & Industry. 1998 ; 3 : 80-8.
  12. Kim HW, Kim KY, Lee SY, Kim GY, Jeon BG, Cho SI, Jeong HW. Immuno-stimulating effects of Oga-Power (OP) containing extract of Acanthopanax sessiliflorus on Immune cells in mice. Kor. J. Herbology. 2008 ; 23(3) : 141-7.
  13. Park SD, Lee GH, Lee YS, Kwon YK, Park JH, Chou SM, Shin SW. Comparison of immunomodulaltory effects of water-extracted Adenophorae Radix, Liriopis Tuber, Dendrobii Herba, Polygonati Odorati Rhizoma and Polygonati Rhizoma. Korean J. Oriental & Pathology. 2007 ; 21(2) : 414-24.
  14. Lee SH, Sung GD, Baek YH. The effects of 10 weeks Taekwondo training with leek intake on body composition, liver enzymes and immune function in high school male students. J. Korean Soc. Living Environ. Sys. 2013 ; 20(1) : 19-28.
  15. Gereltuya R, Son JY, Magsar U, Paik SH, Lee JY, Nam MS. Fermentation properties and inflammatory cytokines modulating of fermented milk with Curcuma longa L powder. Journal of Life Science. 2015 ; 25(1) : 75-83. https://doi.org/10.5352/JLS.2015.25.1.75
  16. Lee SD, Kim KS. The effect of Achyranthis Radix and Apitoxin Aquaacupuncture on cellular immune responses to LPS-induced arthritis in mice. The Acupuncture. 1999 ; 16(3) : 287-315.
  17. Na CS, Shin W, Lee YM, Moon YS, Noh HK, Seo SH, Son HS. Effect of original kyungokgo & iksuyongjingo plus Sparassis crispa on antioxidant, immunity improvement and sensory evaluation. Kor. J. Herbol. 2016 ; 31(4) : 43-51. https://doi.org/10.6116/kjh.2016.31.4.43.
  18. Roitt IM, Brostoff J, Male DK. Immunology 2nd ed., Churchil Livingstone, London, 1.3-3.10. 1989.
  19. Oh JS, Ham IH, Kim HM, Choi HY. Enhancement of immune activities of Cistanche deserticolae Herba and Cistanche salsae Herba. Kor. J. Herbol. 2005 ; 20(1) : 63-72.
  20. Kim MH, Kim JH, Ahn HJ. Responses of proinflammatory and anti-Inflammatory cytokines with clonidine premedication in patients undergoing spinal surgery. Korean J Anesthesiology. 1998 ; 35 : 1080-8. https://doi.org/10.4097/kjae.1998.35.6.1080
  21. Park DJ, Lee JC, Lee YC, Effect of Sinapis alba L. on expression of interferon-gamma and interleukin-4 production in anti-CD3/anti-CD28-stimulated CD4(+) T cells. Kor. J. Herbol. 2010 ; 25(2) : 129-36.
  22. Raishekhar SA, Ashok Khar. Interleukin-12 secreted by mature dendritic cells mediates activation of NK cell function. FEBS letters, 2004 ; 559(1-3) : 71-76. https://doi.org/10.1016/S0014-5793(04)00026-2
  23. Park JH, Seo YB, Experimental studies on the immunnomodulational effects of Taxilli Ramulus. Kor. J. Herbol. 2001 ; 16(1) : 55-71.