Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Cathepsin B & D and the Survival of Early Embryos in Red Spotted Grouper, Ephinephelus akaara

  • Gwon, Seo-Hui (Dept. of Aquatic Life Medical Science, Sunmoon University) ;
  • Kim, Hyun Kyu (Dept. of Aquatic Life Medical Science, Sunmoon University) ;
  • Baek, Hea Ja (Dept. of Marine Biology, Pukyong National University) ;
  • Lee, Young-Don (Dept. of Marine Science Institute, Jeju National University) ;
  • Kwon, Joon Yeong (Dept. of Aquatic Life Medical Science, Sunmoon University)
  • Received : 2017.11.18
  • Accepted : 2017.12.02
  • Published : 2017.12.31
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Abstract

Survival of embryos largely depends on yolk processing during early development. Proteolytic enzymes, cathepsin B & D (ctsb & ctsd) are known to have some important roles in yolk processing of various fish species. Mature female red spotted groupers were injected with human chorionic gonadotropin (HCG) to induce ovulation. The fertilized eggs and embryos were sampled at 0, 4 and 24 HPF (hours post fertilization). Survivals of each groups of embryos were checked at 24 and 48 HPH (hours post hatching). Transcripts of ctsb & ctsd showed the highest level at 0 HPF and relatively high at 4 HPF, but greatly decreased at 24 HPF. In bad egg quality group (BE, embryos survived until 24 HPH), transcript level of ctsb at 4 HPF were significantly lower than the transcript level at the same stage in good egg quality group (GE, embryos survived until 48 HPH) while no significant change of ctsb transcript level was observed at 0 or 24 HPF between BE and GE. Transcript level of ctsd was decreased at 24 HPF, but the difference was not as strong as the case of ctsb transcript. These results suggest that maternal ctsb transcript rather than ctsd transcript is likely to be involved in egg quality resulting in the difference of survival rate of embryos at early developmental period in this species.

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References

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