The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Effects of Lonicerae Caulis (LC) on Allergic Contact Dermatitis (ACD) induced by DNCB in mice

인동등(忍冬藤)이 DNCB로 유발된 알레르기성 접촉피부염에 미치는 영향

  • Lee, Yu-Jin (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Lee, Ho-Chan (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Choi, Jung-Hwa (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Kim, Jong-Han (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Park, Soo-Yeon (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University)
  • 이유진 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 이호찬 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 최정화 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 김종한 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 박수연 (동신대학교 한의과대학 안이비인후피부과학교실)
  • Received : 2017.07.11
  • Accepted : 2017.08.08
  • Published : 2017.08.25
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Abstract

Objectives : Allergic contact dermatitis(ACD) is a type IV delayed hypersensitivity reaction that results from cumulative exposures and subsequent sensitization to an environmental chemical. Lonicerae Caulis(LC) can clear away heat and relieve toxin, disperse wind and heat, dredge the channel. The present study was designed to investigate the effects of LC on allergic contact dermatitis(ACD) induced by 2,4-dinitrochlorobenzene(DNCB) in mice. Methods : In this experiment, the effects of LC on changes in body weights, ear and dorsum skin thicknesses, ear weights, clinical aspects on the dorsum skin, histopathological changes, spleen/body ratio, cytokines were investigated. In addition, the effects on proliferations of splenocytes were also investigated in vitro and vivo study. Results : LC spread(SPR) group and LC spread and administered(SPR+ADM) group showed diminished ear thicknesses. In SPR+ADM group, ear weights were lowered significantly compared to contact dermatitis control(CTL) group. LC treatment diminished erythema, desquamation and keratosis which were induced by repeated painting of DNCB. In histopathological observation, spongiosis and edema were diminished in SPR and SPR+ADM group. In cytokines, SPR+ADM group were increased in IL-10, and SPR and SPR+ADM group were decreased in TNF-${\alpha}$ compared with control group. Conclusions : These data suggest that LC can decrease symptoms of ACD, then LC is useful to treat patient with ACD.

Keywords

References

  1. Korean Dermatological Association Textbook Compilation Committee. Text of Dermatology. 5th ed. Seoul:Yeomoongak. 2008:63-84, 165-70,179-85.
  2. Ahn SG, Jang GH, Song JW, Cheon SH. Common Skin Diesease. Seoul:Doctor's book. 2009:173-90.
  3. Jeon JH, Kim HA, Kang YH. The literature study on Contact dermatitis. The Journal of Oriental Medical surgery, Ophthalmology, Otolaryngology. 1997;10(1):263-83.
  4. So WB. Sossiceypyengwenhwulon. Seoul: Daesung P CO. 1992:258.
  5. Jin SG. Oegwajeongjong. Beijing:Inminwisaengchulpansa. 1983:259.
  6. Oh G. Uijonggeumgam. Shenyang:Liaonínggwahakgisulchulpansa. 1997:649,693,698.
  7. Jin ST. Dongcheonchongnok. Beijing:Inminwisaengchulpansa. 1999:148.
  8. Zhao J. Seongjechongnok. Beijing:Inminwisaengchulpansa. 1995:1577-9,1610-1.
  9. Jeon KW. Junguioegwahak. BeiJing:Junguigojeokchulpansa. 1994:340-2.
  10. Jung GM. Allergy and Korean medicine. Seoul:Jeillo. 1993:46-9,120-1.
  11. Jeon JH, Kim HA, Kang YH. The literature study on Contact dermatitis. The Journal of Oriental Medical surgery, Ophthalmology, Otolaryngology. 1998;11(1):197-218.
  12. Textbook publishing Committee of Korean Medicine. Bonchohak. Seoul:Younglimsa. 2004:267-8.
  13. Lee YC, Kwon TH, Ok IS, Seo CW, Kim YJ, Roh SS, et al. The Experimental Studies on the Immunomodulational Effects of Lonicerae Caulis et Folium(2). Kor J Herbology. 2005;20(4):141-9.
  14. Lee SJ, Shin EJ, Son KH, Chang HW, Kang SS, Kim HP. Anti-inflammatory Actibity of the Major Constituents of Lonicera japonica. Archives of Pharmacal Research. 1995;18(2):133-5. https://doi.org/10.1007/BF02979147
  15. Lee SJ, Son KH, Chang HW, Kang SS, Park PU, Kawk WJ, et al. Development of Plant Anti-inflammatory Agents: Comparison of Anti-inflammatory and Analgesic Activities of Extracts from Lonicera japonica. Kor J Pharmacogn. 1994;25(4):363-7.
  16. Ryu KH, Han CK, Rhee HI, Kim TS, Jung IH, Lee SJ, et al. Anti-asthmatic Activities of the Extract of Lonicera japonica. Kor J Pharmacogn. 1999;30(4):377-83.
  17. Yuan XY, Liu W, Zhang P, Wang RY, Guo JY. Effects and mechanisms of aloperine on 2, 4-dinitrofluorobenzene-induced allergic contact dermatitis in BALB/c mice. European Journal of Pharmacology. 2010;629(1):147-52. https://doi.org/10.1016/j.ejphar.2009.12.007
  18. Wysocki LJ, Sato VL. Planning for lymphocytes:a method for cell selection. Proceedings of the National Academy of Sciences of the United States of America. 1978;75(6):2844-8. https://doi.org/10.1073/pnas.75.6.2844
  19. Mizel SB, Rosenstreich D. Regulation of Lymphocyte-Activating Factor (LAF) Production and Secretion in P388D1 Cells: Identification of High Molecular Weight Precursors of LAF. J Immunol. 1979;122(6):2173-9.
  20. Stepaniak JA, Gould KE, Sun D, Swanborg RH. A comparative study of experimental autoimmune encephalomyelitis in Lewis and DA rats. J Immunol. 1995;155(5):2762-9.
  21. Rivera DL, Olister SM, Liu X, Thompson JH, Zhang X, Pennline K., et al. Interleukin-10 attenuates experimental fetal growth restriction and demise. The FASEB Journal. 1998;12(2):189-97. https://doi.org/10.1096/fasebj.12.2.189
  22. Okura Y, Takeda K, Honda S, Hanawa H, Watanabe H, Kodama M, et al. Recombinant murine interleukin-12 facilitates induction of cardiac myosin-specific type 1 helper T cells in rats. Circ Res. 1998;82(10):1035-42. https://doi.org/10.1161/01.RES.82.10.1035
  23. Lobell A, Weissert R, Storch MK, Svanholm C, de Graaf KL, Lassmann H, et al. Vaccination with DNA encoding an immunodominant myelin basic protein peptide targeted to Fc of immunoglobulin G suppresses experimental autoimmune encephalomyelitis. The Journal of Experimental Medicine. 1998;187(9):1543-8. https://doi.org/10.1084/jem.187.9.1543
  24. Kim MH, Kim MC, Park JS, Kim JW, Lee JO. The antioxidative effects of the water-soluble extracts of plants used as tae materials. Korean J Food Sci Technol. 2001;33:12-8.
  25. Chio KU, Paek DM. Asthma and air polltion in Korea. Korean J Eidemiology. 1995;17:64-75.
  26. Lee JH, Kim KH, Kim MN, Kim JW, Ro YS, Park YL, et al. Report from ADRG: The Treatment Guideline of Korean Atopic Dermatitis. Korean J Dermatol. 2006;44(8):907-13.
  27. Heo J. Donguibogam. Gyeongnam hapcheon: Donguibogamchulpansa. 2005;196,1138,1595.
  28. Kim JA, Kim DK, Kang OH, Choi YA, Park JH, Choi SC, et al. Inhibitory effect of luteolin on TNF-${\alpha}$-induced IL-8 production in human colon epithelial cells. International Immunopharmacology. 2005;5(1):209-17. https://doi.org/10.1016/j.intimp.2004.09.027
  29. Tae J, Han SW, Yoo JY, Kim JA, Kang OH, Baek OS, et al. Anti-inflammatory effect of Lonicera japonica in proteinase-activated receptor 2-mediated paw edema. Clinica Chimica Acta. 2003;330(1-2):165-71. https://doi.org/10.1016/S0009-8981(03)00017-2
  30. Ha TK, Lee YC. Immunomodulatory effects of chlorogenic acid and ethyl acetate fraction from Lonicera japonica on cytokine gene expression profiles in spleen and thymus. Kor J Herbology. 2011;26(2):1-10. https://doi.org/10.6116/KJH.2011.26.2.001
  31. Kim SS. Anti-cancer and whitening effects of Lonicerae Caulis (LC) in melanoma cells. Graduate school of Dongshin University. 2010.
  32. Kim JK, Kim YH, Lee HH, Lim SS, Park KW. Effect of Scrophularia buergeriana Extract on the Degranulation of Mast Cells and Ear Swelling Induced by Dinitrofluorobenzene in Mice. Inflammation. 2012;35(1):183-91. https://doi.org/10.1007/s10753-011-9304-x
  33. Saint MP, Rosieres A, Krasteva M, Berard F, Dubois B, Kaiserlian D, et al. Allergic contact dermatitis. European Journal of Dermatology. 2004;14(5):284-95.
  34. Burk RL, Ackerman N. Small animal radiology and ultrasonography: a diagnostic atlas and text. 2nd ed. Philadelphia: WB Saunders Co. 1996:410-4.
  35. Jung IJ, Wang JH, Yun JW, Lee KW, Yeon SC, Lee HJ, et al. Radiographic Estimation of Experimentally Induced Splenomegaly in Dogs. J Vet Clin. 2006;23(3):263-6.
  36. Seo YB, Lee YC. Effects on Ethyl Acetate Fraction of ELC on Highly Purivied Mice T cells. Kor J Herbology. 2003;18(3):113-29.
  37. Stasiuk LM, Abehsira AO, Fournier C. Collagen-Induced Arthritis in DBA/1 Mice: Cytokine Gene Activation Following Immunization with Type II Collagen. Celluar Immunol. 1996;173(2):269-75. https://doi.org/10.1006/cimm.1996.0277
  38. Dayer JM, Rochemonteix BD, Burres B, Demczuk S, Dinarello CA. Human recombinant interleukin 1 stimulates collagenase and prostaglandin E2 production by human synovial cells. J Clin Invest. 1986;77(2):645-8. https://doi.org/10.1172/JCI112350
  39. Corsini E, Galli CL. Epidermal cytokines in experimental contact dermatitis. Toxicology. 2000;142(3):203-11. https://doi.org/10.1016/S0300-483X(99)00145-6
  40. Maeda A, Schwarz A, Bullinger A, Morita A, Peritt D, Schwarz T. Experimental extracorporeal photopheresis inhibits the sensitization and effector phases of contact hypersensitivity via two mechanisms: generation of IL-10 and induction of regulatory T cells. J Immunol. 2008;181(9):5956-62. https://doi.org/10.4049/jimmunol.181.9.5956