Journal of Korean society of Dental Hygiene (한국치위생학회지)

Korean Society of Dental Hygiene (한국치위생학회)
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Effect of Cimicifuga rhizoma extract on the odontoblastic differentiation of MDPC-23 cells

승마추출물이 MDPC-23세포의 분화에 미치는 영향

  • Choi, Byul-Bora (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Kim, Ji-Young (Department of Dental Hygiene, Kyungnam College of Information & Technology) ;
  • Park, Sang-Rye (Department of Dental Hygiene, Kyungnam College of Information & Technology)
  • 최별보라 (부산대학교 치의학전문대학원 구강해부학교실) ;
  • 김지영 (경남정보대학교 치위생과) ;
  • 박상례 (경남정보대학교 치위생과)
  • Received : 2017.04.04
  • Accepted : 2017.05.22
  • Published : 2017.06.30
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Abstract

Objectives: The purpose of this study was to examine the cell proliferation and expression of alkaline phosphatase (ALP) during the differentiation of murine odontoblast-like cells (MDPC-23) by Cimicifuga rhizoma extract. Cimicifuga rhizoma extract was prepared using 70% ethanol. Then, the cells were treated with 25, 50, 100, 150, and $200{\mu}g$ of Cimicifuga rhizoma extract. Methods: We determined the Cimicifuga rhizoma effects of MDPC-23 using WST-1 (water soluble tetrazolium salt-1) assay, ALP activity assay and histochemical staining. Results: $25-200{\mu}g$ of Cimicifuga rhizoma extract did not inhibit the growth of MDPC-23 cells; $100{\pm}0$, $100{\pm}3.29$, $99{\pm}4.86$, $98{\pm}3.80$, $98{\pm}1.73$, $99{\pm}5.05%$ (p<0.794). $50{\mu}g$ of Cimicifuga rhizoma extract stimulated ALP activity on MDPC-23; $5.1{\pm}0.20units/{\mu}{\ell}$ (p<0.001). Conclusions: It was proven that Cimicifuga rhizoma promoted differentiation of MDPC- 23 cells.

Keywords

References

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