Journal of the Korea Convergence Society (한국융합학회논문지)

Korea Convergence Society (한국융합학회)
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Observation of the Structure of Melanin Granule in Mummy's Hair Shaft Using High-Voltage Electron Microscopy

초고압전자현미경을 이용한 미라 모발 멜라닌과립의 구조 관찰

  • 장병수 (한서대학교 피부미용향장화학과)
  • Received : 2017.06.22
  • Accepted : 2017.08.20
  • Published : 2017.08.28
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Abstract

Three-dimensional melanin granules of mummy's hair analyzed using high voltage electron microscopy at 1250kV. The melanin granules in the cortex of mummy's hair located in close proximity to the outer of cortex in the adjacent place of the cuticles. A lot of melanin granule performed in this area. It is a distinguished difference from modern human. While it rotated up to 60 degree counter-clockwise and taken a photo per one degree using high voltage electron microscopy. The melanin granule displayed with long elliptical and variable at the size. The size of granule is measured from $0.3{\sim}0.6{\mu}m$ in minimum diameter and $0.5{\sim}1{\mu}m$ in maximum diameter. Conclusionally, high voltage electron microscope has higher resolution and penetration power than conventional transmission electron microscope that could be load biological thick specimen.

본 연구는 미라 모발에 존재하는 멜라닌과립의 보존 상태와 모양을 규명하기 위해서 1250kV의 초고압전자현미경(high voltage electron microscope: HVEM)을 사용하여 3차 구조를 분석하였다. 미라 모발에서 피질에 분포하고 있는 멜라닌과립은 큐티클층에 인접한 바깥쪽 피질에 집중적으로 분포하고 있다. 이들 부위에는 다량의 멜라닌과립들이 무리를 지어 존재하고 있어서 현대인의 정상 모발에 있는 멜라닌과립의 분포양상과 뚜렷한 차이점을 나타내었다. 초고압전자현미경으로 ${\pm}60$도의 범위에서 1도 간격으로 촬영한 결과 멜라닌과립은 길쭉한 타원형의 형태로 크기가 다양하게 관찰되었다. 과립의 크기는 단축 직경이 $0.3-0.6{\mu}m$이고, 장축 직경은 $0.5-1{\mu}m$로 측정되었다. 결론적으로 초고압전자현미경은 일반 투과전자현미경보다 높은 해상도와 투과력을 가져서 두꺼운 절편의 생물 시료 구조분석에 용이하다.

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References

  1. Y. S. Kim, I. S. Lee, G. U. Jung, M. J. Kim, C. S. Oh, D. S. Yoo, W. J. Lee, E Lee, S. C. Cha, D. H. Shin, "Radiological Diagnosis of Congenital Diaphragmatic Hernia in 17th Century Korean Mummy". PLoS One, Vol. 9, No. 7, DOI: 10.1371/journal.pone.0099779, 2014.
  2. D. H. Shin, Y. H. Choi, K. J. Shin, G. R. Han, M. Youn, C. Y. Kim, S. H. Han, J. C. SEO, S. S. Park, Y. J. Cho, B. S. Chang, "Radiological analysis on a mummy from a medieval tomb in Korea". Annals of Anatomy. Vol. 185, No. 4, pp: 377-382, 2003. https://doi.org/10.1016/S0940-9602(03)80065-1
  3. D. H. Shin, M. Youn, B. S. Chang, "Histological analysis on the medieval mummy in Korea". Forensic Science International, Vol, 137, No. 26, pp. 172-182, 2003, https://doi.org/10.1016/S0379-0738(03)00335-9
  4. D. H. Shin, C. S. Oh, S. J. Lee, E,,J, Lee, M, Seo, "Ectopic paragonimiasis from 400-year-old female mummy of Korea" Journal of Archaeological Science, Vol. 39, No. 4, pp. 1103-1110. 2012. https://doi.org/10.1016/j.jas.2011.12.011
  5. I. S. Lee, M. J. Kim, D. S. Yoo, Y. S. Lee, D. H. Shin, "Three-dimensional reconstruction of medieval child mummy in Yangju, Korea, using multi-detector computed tomography" Annals of Anatomy, Vol. 189, No. 6, pp. 558-568, 2007. https://doi.org/10.1016/j.aanat.2007.03.005
  6. G. Y. Lee, B. S. Chang, "Studies on the preservative condition and the ultrastracture of hair of newly found sixteenth century mummy in Paju". Korean Journal of Electron Microscopy Vol. 35, No. 4, pp. 211-218, 2005.
  7. B. S. Chang, C. S. Uhm, C. H. Park, H. K. Kim, G. Y. Lee, H. H. Cho, Y. H. Chung, D. H. Shin, "Ultramicroscopic study on the hair of newly found 15th century mummy in Daejeon, Korea". Annals of Anatomy, Vol. 188, No. 5, pp. 5439-45, 2006.
  8. Korea university museum. "Papyeong Yun Ssi Mira Jonghap Yeongu Nonmun Jip". Korea university museum: Seoul Korea. pp. 205-230, 2003. (Korean)
  9. B. S. Chang, C. S. Uhm, C. H. Park, H. K. Kim, G. Y. Le, H. H. Cho, M. J. Kim, Y. H. Chung, K. W. Song, D. S. Lim, D. H. Shin, "Preserved skin structure of a recently found fifteenth-century mummy in Daejeon, Korea". Journal of Anatomy, Vol. 209, No. 5, pp. 671-680, 2006. https://doi.org/10.1111/j.1469-7580.2006.00607.x
  10. B, S, Chang, "Analysis on the preservation of scalp collected from full-term baby mummy of medieval Joseon Dynasty". Applied Microscopy, Vol. 38, No. 2, pp.135-140, 2008.
  11. J. R. Kremer, D. N. Mastronarde, J. R. McIntosh, "Computer visualization of the three-dimensional image data using IMOD". Journal of Structural Biology, Vol. 116, No. 1, pp. 71-76, 1996. https://doi.org/10.1006/jsbi.1996.0013
  12. J. Y. Lee, B. S. Chang, "Morphogenic types of cuticles and melanin granules in the anagen phase of hair cuticles". Scientific Research and Essays, Vol. 8, No. 20, pp. 807-814, 2013.
  13. K. S. Kim, B. S. Chang, "Keratinization of the cuticle layer of hair follicles during the anagen phase". Journal of Investigative Cosmetology, Vol. 13, No. 1, pp. 43-47, 2017. https://doi.org/10.15810/jic.2017.13.1.007
  14. G. Y. Lee, B. S. Chang, "General characteristics on the fine structure of scalp hair -I. Ultrastructure of hair shaft-", Journal of Investigative Cosmetology, Vol. 6, No. 3, pp. 265-271, 2010. https://doi.org/10.15810/jic.2010.6.3.006
  15. B. S. Chang, "Study on the ultrastructural characteristics of human hair medulla". Journal of the Korea Convergence Society, Vol 8, No. 7, pp. 123-129, 2017. https://doi.org/10.15207/JKCS.2017.8.7.123
  16. J. S. Lee, J. A. Nor, B. S. Chang, "Ultrastructural characteristics on the cuticle layer according to different thickness of hairs". Journal of Investigative Cosmetology, Vol. 11, No. 1, pp. 81-87, 2015. https://doi.org/10.15810/jic.2015.11.1.010
  17. K. H. Lee, G. Y. Lee, M. S. Kim, G. E. Kim, Y. K. Kim, B. S. Chang, "Morphological study of the bleaching agent and bleaching hair".Journal of Investigative Cosmetology, Vol. 2, No. 3, pp. 79-89, 2006.
  18. G. Y. Lee, B. S. Chang, "Ultrastructural changes of hair cuticle layer treated with permanent dye". Journal of Investigative Cosmetology, Vol.7, No. 3, pp. 267-271, 2011. https://doi.org/10.15810/jic.2011.7.3.005
  19. J. A. Rho, T. B. Choi, B. S. Chang, "Ultrastructural analysis on the straight permed hair". Journal of Investigative Cosmetology, Vol. 6, No. 1, pp.41-47, 2010. https://doi.org/10.15810/jic.2010.6.1.006
  20. G. Y. Lee, B. S. Chang, "Electron microscopic studies on the morphological differences of ethnic hair. Korean Journal of Electron Microscopy Vol. 38, No. 3, pp. 265-273, 2008.
  21. S. Y. Kang, K. A. Lee, "A study of convergence management types based on self perception of scalp and hair among college students majoring in cosmetology". Journal of the Korea Convergence Society, Vol 8, No. 2, pp. 235-241, 2017. https://doi.org/10.15207/JKCS.2017.8.2.235
  22. H. W. Kim, J. W. Kim, I. M. Rhyu, "The high-voltage electron microscopy in biomedical research. Korean Journal of Anatomy, Vol. 42, No. 2, pp. 73-81, 2009.
  23. J. W. Kim, S. J. Lee, I. M. Rhyu, "Construction of anaglypic stero pair image using adobe photoshop program". Korean Journal of Electron microscopy, Vol. 36, No. 3, pp. 143-146, 2007.
  24. H. W. Kim, S. T. Hong, S.H. Oh, C. H. Park, H. Kim, I. J. Rhyu, "Whole mount preparation of primary cultured neuron for HVEM observation". Applied Microscopy, Vol. 41, No.1, pp. 69-73. 2011.