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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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SRSF2 directly inhibits intron splicing to suppresses cassette exon inclusion

  • Moon, Heegyum (School of Life Sciences, Gwangju Institute of Science and Technology) ;
  • Cho, Sunghee (School of Life Sciences, Gwangju Institute of Science and Technology) ;
  • Loh, Tiing Jen (School of Life Sciences, Gwangju Institute of Science and Technology) ;
  • Jang, Ha Na (School of Life Sciences, Gwangju Institute of Science and Technology) ;
  • Liu, Yongchao (School of Life Sciences, Gwangju Institute of Science and Technology) ;
  • Choi, Namjeong (School of Life Sciences, Gwangju Institute of Science and Technology) ;
  • Oh, Jagyeong (School of Life Sciences, Gwangju Institute of Science and Technology) ;
  • Ha, Jiyeon (School of Life Sciences, Gwangju Institute of Science and Technology) ;
  • Zhou, Jianhua (JiangSu Key Laboratory of Neuroregeneration, Nantong University) ;
  • Cho, Sungchan (Bio-Therapeutics Research Institute, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Dong-Eun (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Ye, Michael B. (Division of Liberal Arts and Sciences, Gwangju Institute of Science and Technology) ;
  • Zheng, Xuexiu (School of Life Sciences, Gwangju Institute of Science and Technology) ;
  • Shen, Haihong (School of Life Sciences, Gwangju Institute of Science and Technology)
  • Received : 2017.06.16
  • Accepted : 2017.07.17
  • Published : 2017.08.31
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Abstract

SRSF2, a Serine-Arginine rich (SR) protein, is a splicing activator that mediates exon inclusion and exclusion events equally well. Here we show SRSF2 directly suppresses intron splicing to suppress cassette exon inclusion in SMN pre-mRNA. Through a serial mutagenesis, we demonstrate that a 10 nt RNA sequence surrounding the branch-point (BP), is important for SRSF2-mediated inhibition of cassette exon inclusion through directly interacting with SRSF2. We conclude that SRSF2 inhibits intron splicing to promote exon exclusion.

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References

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