Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Isolation and characterization of cholesterol-lowering lactic acid bacteria from kimchi

김치에서 분리된 콜레스테롤 감소능을 가진 젖산세균의 특성

  • Park, Hong-Yeop (Department of Food Science and Technology, Pukyong National University) ;
  • Park, Seul-Ki (Department of Food Science and Technology, Pukyong National University) ;
  • Kim, Bo-Geum (Department of Food Science and Technology, Pukyong National University) ;
  • Ryu, Dae-Gyu (Department of Food Science and Technology, Pukyong National University) ;
  • Lim, Eun-Seo (Department of Food Science and Nutrition, Tongmyong University) ;
  • Kim, Young-Mog (Department of Food Science and Technology, Pukyong National University)
  • Received : 2017.03.24
  • Accepted : 2017.05.18
  • Published : 2017.08.31
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Abstract

The objective of this study was to isolate and characterize lactic acid bacteria (LAB) exhibiting cholesterol-lowering activity from the Korean traditional fermented food, kimchi. The previously isolated LAB strains were assessed for cholesterol-lowering efficacy in the presence of 0.1% cholesterol. All LAB strains tested in this study were able to assimilate cholesterol at varying levels, ranging from 35.0 to 99.4%. Among them, the Lactobacillus plantarum FMB 31 strain exhibited the highest cholesterol-lowering effect with 99.4% cholesterol removal efficiency. The strain was stable in the presence of acid, bile, and salt stress, and showed high adherence on HT-29 cells, a human colon line. In addition, the LAB strain showed no pathogenic properties such as the production of hemolysin and biogenic amines. Thus, this study suggests that the L. plantarum FMB 31 strain isolated from kimchi can be a potential source of probiotic products with strong cholesterol-lowering effect.

본 연구에서는 한국 전통식품인 김치에서 분리된 LAB를 이용하여 콜레스테롤 감소능을 가지고 있는 LAB를 분리하고 그 특성을 규명하였다. 23 종의 분리 LAB 중에서 L. plantarum FMB 31 균주가 99.4%의 높은 콜레스테롤 감소 활성을 나타내었고, 프로바이오틱스로서 활용하기 위한 중요한 특성인 내담즙성, 내산성 그리고 내염성 등이 대조구로 사용된 L. rhamnosus KCTC 5033 균주보다 우수하거나 동등하였다. 또한 L. plantarum FMB 31 균주는 인체 안전성과 관련된 용혈독성 및 생체아민 생성능 실험에서도 안전성에 문제가 없는 것으로 분석되었다. 향후, 이 분리균은 콜레스테롤 저하 기능을 가지는 다양한 건강 기능 식품 개발에 이용될 수 있을 것으로 기대된다.

Keywords

References

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