Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Ameliorating effect of the ethyl acetate fraction of Pteridium aquilinum on glucose-induced neuronal apoptosis

포도당으로 유도된 신경세포 손상에 대한 고사리 아세트산에틸 분획물의 개선 효과

  • Park, Seon Kyeong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Guo, Tian Jiao (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Jong Min (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kang, Jin Yong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Sang Hyun (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kang, Jeong Eun (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kwon, Bong Seok (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Chang Jun (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Uk (Division of Special Purpose Tree, National Institute of Forest Science) ;
  • Heo, Ho Jin (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
  • 박선경 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원) ;
  • 궈텐쟈오 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원) ;
  • 김종민 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원) ;
  • 강진용 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원) ;
  • 박상현 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원) ;
  • 강정은 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원) ;
  • 권봉석 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원) ;
  • 이창준 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원) ;
  • 이욱 (국립산림과학원 특용자원연구과) ;
  • 허호진 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원)
  • Received : 2017.02.28
  • Accepted : 2017.05.09
  • Published : 2017.08.31
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Abstract

The protective effect of Pteridium aquilinum on high glucose-induced cytotoxicity was examined in vitro to investigate the relationship between diabetic condition and neuronal dysfunction. The ethyl acetate fraction of P. aquilinum (EFPA), with total phenolic content of 265.08 mg gallic acid equivalent/g, showed higher 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)/2,2-diphenyl-1-picrylhydrazyl radical scavenging activities and lipid peroxidation inhibitory effect than any other fraction. In addition, EFPA showed a significant reduction in the inhibitory effect on ${\alpha}$-glucosidase activity ($IC_{50}$ value=$205.26{\mu}g/mL$) compared to the acarbose positive control. The anti-oxidative effect in PC12 cells, protective effects on high glucose-induced oxidative stress in neuronal cells, and neurotoxicity were measured using 2',7'-dichlorofluorescin diacetate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide, and lactate dehydrogenase assays, respectively. EFPA showed conspicuous inhibitory effect on cellular reactive oxygen species production and neuronal cell apoptosis. Finally, kaempferol-3-glucoside was identified as the main phenolic compound of EFPA using high performance liquid chromatography.

고사리(Pteridium aquilinum)의 4가지 분획물(n-헥산, 클로로포름, 아세트산에틸 및 증류수)의 총 페놀 함량은 아세트산에틸 분획물이 265.08 mg GAE/g으로 가장 높게 나타났으며, 라디칼 소거활성 및 지질 과산화 생성 억제효과 결과 또한 아세트산에틸 분획물의 활성이 가장 높게 나타났다. 고사리의 아세트산에틸 분획물은 알파-글루코시데이스에 대하여 뛰어난 억제활성($IC_{50}=205.26{\mu}g/mL$)을 나타내었으며, 억제 형태는 혼합형 제해 특성임을 확인하였다. 또한, PC12 신경세포에서의 고농도 포도당으로 유도된 산화적 스트레스에 대한 고사리 아세트산에틸 분획물은 세포 내 ROS 생성을 억제시키고, 세포 생존율을 증가 및 세포막 손상에 대한 보호효과를 나타내었다. 고사리 아세트산에틸 분획물의 주요 페놀성 화합물을 HPLC로 분석한 결과는 켐페롤-3-글루코사이드인 것으로 나타났다. 본 연구 결과를 종합해볼 때, 주요 페놀성 물질로서의 켐페롤-3-글루코사이드을 함유한 고사리 아세트산에틸 분획물은 소장 내 알파-글루코시데이스를 억제를 통한 혈당 강하 효과뿐만 아니라, 고농도 포도당으로 발생되는 산화적 스트레스에 대한 억제효과를 통하여 신경세포 사멸에 대한 효과적인 개선 효과를 갖는 고부가가치 자연 소재로의 활용가치가 높다고 판단된다.

Keywords

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