Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Study of optimization of natural nitrite source production from spinach

시금치 유래 천연 아질산염 생산의 최적화 연구

  • Kim, Tae-Kyung (Food Processing Research Center, Korea Food Research Institute) ;
  • Seo, Dong-Ho (Food Processing Research Center, Korea Food Research Institute) ;
  • Sung, Jung-Min (Food Processing Research Center, Korea Food Research Institute) ;
  • Ku, Su-Kyung (Food Processing Research Center, Korea Food Research Institute) ;
  • Jeon, Ki-Hong (Food Processing Research Center, Korea Food Research Institute) ;
  • Kim, Young-Boong (Food Processing Research Center, Korea Food Research Institute) ;
  • Choi, Yun-Sang (Food Processing Research Center, Korea Food Research Institute)
  • 김태경 (한국식품연구원 식품가공기술연구센터) ;
  • 서동호 (한국식품연구원 식품가공기술연구센터) ;
  • 성정민 (한국식품연구원 식품가공기술연구센터) ;
  • 구수경 (한국식품연구원 식품가공기술연구센터) ;
  • 전기홍 (한국식품연구원 식품가공기술연구센터) ;
  • 김영붕 (한국식품연구원 식품가공기술연구센터) ;
  • 최윤상 (한국식품연구원 식품가공기술연구센터)
  • Received : 2017.02.08
  • Accepted : 2017.03.06
  • Published : 2017.08.31
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Abstract

This study investigated the screening and optimization of nitrite production from fermented spinach extract using different lactic acid bacteria, fermentation temperature, and time. Spinach extract was fermented using various lactic acid bacteria at 24, 30, and $36^{\circ}C$ for 6, 12, 18, 24, 36, 48, 72, and 96 h in the presence of different carbohydrates (glucose, sucrose, fructose, and lactose). Lactobacillus farciminis (KCTC 3618) produced the highest amount of nitrite using fermented spinach extract at $30^{\circ}C$ for 28 h compared to Staphylococcus carnosus, L. coryniformis (KCTC 3167), L. fructosus (KCTC 3544), L. reuteri (KCTC 3677), L. amylophilus (KCTC 3160), L. hilgardii (KCTC 3500), L. delbrueckii (KCTC 1058), L. fermentum (KCTC 3112), L. plantarum (KCTC 3104), and L. brevis (KCTC 3498). Comparison of the yield at different fermentation temperatures showed that the highest amount of nitrite was produced using fermented spinach extract at $30^{\circ}C$. Similarly, maximum nitrite yield was observed after 36 h fermentationin in the presence of sucrose. Therefore, maximum nitrite production was observed upon L. farciminis-mediated fermentation of spinach extractat $30^{\circ}C$ for 36 h in the presence of sucrose.

본 연구는 Lactobacillus 표준균주를 활용하여 시금치 발효액의 질산염을 아질산염으로 환원시켜 천연 아질산염 생산량의 최적화 조건을 확인하기 위하여 진행하였다. 유산균 종, 발효온도, 발효시간, 탄소원에 따른 아질산염 생산량에 대하여 평가하였다. 본 연구에서 사용한 Lactobacillus 표준균주에서는 L. farciminis(KCTC 3618)가 가장 많은 아질산염을 생산하였고, 발효온도 및 배양온도는 $30^{\circ}C$에서 36시간이 가장 많은 양의 아질산염을 생산하였다. 설탕을 첨가하였을 때 다른 탄소원을 첨가하였을 경우 보다 많은 양의 아질산염을 생산하였다. 따라서 L. farciminis를 스타터 배양로 활용하여 시금치의 질산염을 아질산염으로 환원($30^{\circ}C$, 36 h)한다면 산업적으로 가장 효과적으로 천연 아질산염을 생산할 수 있을 것으로 사료된다.

Keywords

References

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