Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of different drying methods on anti-oxidation and anti-thrombosis activities of Salicornia europaea

함초의 건조방법에 따른 항산화 및 항혈전 활성의 변화

  • Received : 2016.07.26
  • Accepted : 2017.08.10
  • Published : 2017.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

The current study was performed to investigate the changes of bio-activities of Salicornia europaea (SE) depending on the different dry methods. The ethanol extracts were prepared from the freeze-drying SE (FD), hot-air drying SE (HD), and shade drying SE (SD). Their anti-oxidant and anti-thrombosis activities were compared. The yields of ethanol extraction in FD, HD, and SD were 14.4, 13.2 and 11.9%, respectively. The highest contents of total polyphenol (4.6 mg/g) and total sugar (23.4 mg/g) were shown in FD, whereas, the highest content of reducing sugar (14.6 mg/g) was observed in HD. In anti-oxidation activity assay, the FD and HD showed similar radical scavenging activities and reducing power. However, in SD, nitrite scavenging activity and reducing power were severely decreased. In anti-coagulation activity assay, the thrombin time (TT), prothrombin time (PT) and activated partial thromboplastin time (aPTT) of FD, HD and SD did not show significant changes at 5 mg/mL concentration. But the HD at 7 mg/mL had strong inhibitions against thrombin and blood coagulation factors. The platelet aggregation and hemolysis activities were not affected by dry methods. Our results suggest that both FD and HD are effective to maintain the functional ingredient of SE, and HD is economic and efficient dry process for production of functional food.

식용 및 약용으로 이용되고 있는 함초를 이용한 고부가가치 제품개발을 위해, 동결건조, 열풍건조, 음건한 함초의 ethanol추출물을 조제하여 건조방법에 따른 성분 변화, 항산화 및 항혈전 활성 변화를 평가하였다. 동결건조, 열풍건조 및 음건 시료의 ethanol 추출효율은 각각 14.4, 13.2, 11.9%를 나타내어 동결건조시 수율이 음건에 비해 1.2배 높았다. 성분 분석결과, 동결건조 시료의 total polyphenol 함량은 4.6 mg/g으로 열풍건조 및 음건 시료보다 높았으며, total flavonoid 함량은 건조방법과 무관하게 4.2-4.4 mg/g을 나타내었다. 반면 총당 함량은 동결건조에서 가장 높게 나타났으며, 음건 시료는 동결건조 시료에 비해 53% 수준을 나타내었다. 환원당의 경우 열풍건조시료에서 가장 높게 나타났으며, 음건시료에 비해 3.3배 높게 나타났다. 항산화 활성 평가결과, 동결건조, 열풍건조 시료에서는 유사한 항산화 활성이 나타났으나, 음건 시료의 경우 환원력과 nitrite 소거능은 유의적으로 감소되었다. 항응고 활성의 경우, 모든 건조 시료에서 농도 의존적인 우수한 항응고 활성을 나타내었으며, 특히 열풍건조 시료에서 강력한 TT 및 aPTT 연장 활성을 나타내었다. 인간 혈소판에 대한 응집저해 활성과 적혈구 용혈활성은 건조방법에 따른 유의적인 변화는 나타나지 않았다. 본 연구결과는 함초의 유용 기능성 유지를 위해 열풍건조 및 동결건조가 필요하며, 특히 함초의 항혈전 활성을 이용하는 기능성 식품을 제조하고자 하는 경우 열풍건조를 이용하는 것이 경제적이면서 효과적임을 제시하고 있다.

Keywords

References

  1. Jin TY, Quan WR, Wang MH (2008) Changes of physicochemical and sensory characteristics in the Codonopsis lanceolata Saengsik, uncooked food by different drying methods. Korean J Food Sci Technol, 40, 721-725
  2. Kang NS, Kim JH, Kim JK (2007) Modification of quality characteristics of onion powder by hot-air, vacuum and freeze drying methods. Korean J Food Preserv, 14, 61-66
  3. Lim YR, Kyung YN, Jeong HS, Kim HY, Hwang IG, Yoo SM, Lee JS (2012) Effects of drying methods on quality of red pepper powder. J Korean Soc Food Sci Nutr, 41, 1315-1319 https://doi.org/10.3746/jkfn.2012.41.9.1315
  4. Park SJ, Lee JS, Hoe YH, Moon EY, Kang MH (2008) Physiology activity of barley leaf using different drying methods. J Korean Soc Food Sci Nutr, 37, 1627-1631 https://doi.org/10.3746/jkfn.2008.37.12.1627
  5. Choi IY, Song YJ, Choi DC, Lee WH (2010) A comparative study for obtaining maximum essential oil from six herbs on the basis of harvesting time, cultivation regions & type, and drying methods. Kor J Hort Sci Technol, 28, 492-496
  6. Park SJ, Lee DW, Park SH, Rha YA (2016) Quality characteristics of Cirsium setidens Nakai by different drying method. Culi Sci Hos Res, 22, 104-114
  7. Nam S, Kwon YR, Chung HS, Youn KS (2016) Antioxidant abilities and physiological properties of dried haw extracts prepared using different drying methods. Korean J Food Preserv, 23, 246-251 https://doi.org/10.11002/kjfp.2016.23.2.246
  8. Jeong DS, Youn KS (2016) Comparison of the antioxidant and physiological activities of grape seed extracts prepared with different drying methods. Korean J Food Preserv, 23, 1-6 https://doi.org/10.11002/kjfp.2016.23.1.1
  9. Lee JM (2016) Physicochemical and antioxidant properties in Allium hookeri by hot air-and freeze-drying methods. Korean J Food Preserv, 23, 57-62 https://doi.org/10.11002/kjfp.2016.23.1.57
  10. Choi SI, Lee JH, Cho ML, Shin GH, Kim JM, Oh JW, Jung TD, Rhee SK, Lee OH (2015) Changes of quality characteristics of Jicama (Pachyrhizus erosus) potato powder by drying methods. Korean J Food Preserv, 22, 915-919 https://doi.org/10.11002/kjfp.2015.22.6.915
  11. Lee S, Kim JK (2015) Quality characteristics of Aronia melanocarpa by different drying method. Korean J Food Preserv, 22, 56-62 https://doi.org/10.11002/kjfp.2015.22.1.56
  12. Lee S, Moon HK, Lee SW, Moon JN, Kim JK (2014) Effects of drying methods on quality characteristics and antioxidative effects of Omija (Schizandra chinesis bailon). Korean J Food Preserv, 21, 341-349 https://doi.org/10.11002/kjfp.2014.21.3.341
  13. Park SJ, Choi YB, Ko JR, Rha YA, Lee HY (2014) Effects of drying methods on the quality and physiological activities of blueberry (Vaccinium ashei). Korean J Culinary Res, 20, 55-64
  14. ,Lee JH Son SM, Kw on HO(2011) Physicochemical composition of Capsosiphon fulvescens according to drying methods. J Korean Soc Food Sci Nutr, 40, 1582-1588 https://doi.org/10.3746/jkfn.2011.40.11.1582
  15. Lee SJ, Seo JK, Shin JH, Lee HJ, Sung NJ (2008) Antioxidant activity of wa-song (Orostachys japonicus A. Berger) according to drying methods. J Korean Soc Food Sci Nutr, 37, 605-611 https://doi.org/10.3746/jkfn.2008.37.5.605
  16. Cha JY, Jeong JJ, Kim YT, Seo WS, Yang HJ, Kim JS, Lee YS (2006) Detection of chemical characteristics in Hamcho (Salicornia herbacea L.) according to harvest periods. J Life Sci, 16, 683-690 https://doi.org/10.5352/JLS.2006.16.4.683
  17. Jo YC, Ahn JH, Chon SM, Lee KS, Bae TJ, Kang DS (2002) Studies on pharmacological effects of glasswort (Salicornia herbacea L.). Korean J Medicinal Crop Sci, 10, 93-99
  18. Cho YS, Kim SI, Han YS (2008) Effects of slander glasswort (Salicornia herbacea L.) extract on improvements in bowel function and constipation relief. Korean J Food Sci Technol, 40, 326-331
  19. Pichiah PBT, Cha YS (2015) Salicornia herbacea prevents weight gain and hepatic lipid accumulation in obese ICR mice fed a high-fat diet. J Sci Food Agric, 95, 3150-3159 https://doi.org/10.1002/jsfa.7054
  20. Shin MG, Lee GH (2013) Spherical granule production from micronized saltwort (Salicornia herbacea) powder as salt substitute. Prev Nutr Food Sci, 18, 60-66 https://doi.org/10.3746/pnf.2013.18.1.060
  21. Chung YC, Chun HK, Yang JY, Kim JY, Han EH, Kho YH, Jeong HG (2005) Tungtungmadic acid, a novel antioxidant, from Salicornia herbacea. Arch Pharm Res, 28, 1122-1126 https://doi.org/10.1007/BF02972972
  22. Jang HS, Kim KR, Choi SW, Woo MH, Choi JH (2007) Antioxidant and antithrombus activities of enzymetreated Salicornia herbacea extracts. Ann Nutr Metab, 51, 119-125 https://doi.org/10.1159/000100826
  23. Cha JY, Jeon BS, Kim BK, Kang HY, Cho YS (2005) Physiological effect of Hamcho yogurt on streptozotocininduced diabetic rats. J Life Sci, 15, 619-625 https://doi.org/10.5352/JLS.2005.15.4.619
  24. Han EH, Kim JY, Kim HG, Chun HK, Chung YC, Jeong HG (2010) Inhibitory effect of 3-caffeoyl-4-dicaffeoylquinic acid from Salicornia herbacea against phorbol esterinduced cyclooxygenase-2 expression in macrophages. Chem Biol Interact, 183, 397-404 https://doi.org/10.1016/j.cbi.2009.11.015
  25. Kim MS, Lee JN, Seong HJ, Kim DH, Sohn HY (2016) Changes in in vitro antioxidant and antithrombosis activities of Salicornia europaea according to harvest time. J Life Sci, 26, 1033-1040 https://doi.org/10.5352/JLS.2016.26.9.1033
  26. Lee JN, Kim MS, Kim DH, Sohn HY (2016) Anti-oxidation and anti-thrombosis activities of different parts of Salicornia herbacea L. Microbiol Biotechnol Lett, 44, 311-316 https://doi.org/10.4014/mbl.1607.07003
  27. Kong CS, Kim YA, Kim MM, Park JS, Kim JA, Kim SK, Lee BJ, Nam TJ, Seo Y (2008) Flavonoid glycosides isolated from Salicornia herbacea inhibit matrix metalloproteinase in HT1080 cells. Toxicol In Vitro, 22, 1742-1748 https://doi.org/10.1016/j.tiv.2008.07.013
  28. Kim MJ, Jun HY, Kim JH (2015) Anti-obesity effect of Korean Hamcho (Salicornia herbacea L.) powder on high-fat diet-induced obese rats. J Nutr Health, 48, 123-132 https://doi.org/10.4163/jnh.2015.48.2.123
  29. Ryu DS, Kim SH, Lee DS (2008) Immunomodulating activity of Salicornia herbacea extract. Kor J Microbiol Biotechnol, 36, 135-141
  30. Ryu DS, Kim SH, Lee DS (2009) Anti-proliferative effect of polysaccharides from Salicornia herbacea on induction of G2/M arrest and apoptosis in human colon cancer cells. J Microbiol Biotechnol, 19, 1482-1489
  31. Kim MS, Seong HJ, Sohn HY (2016) In-vitro antithrombosis activity of different parts of Sorbus commixta from Ulleung island. J Life Sci, 26, 289-295 https://doi.org/10.5352/JLS.2016.26.3.289
  32. Kim MS, Shin WC, Kang DK, Sohn HY (2016) Anti-thrombosis activity of sinapic acid isolated from the lees of Bokbunja wine. J Microbiol Biotechnol, 26, 61-65 https://doi.org/10.4014/jmb.1508.08095
  33. Sweeney JD, Hoerning LA, Behrens AN, Novak E, Swank RT (1990) Thrombocytopenia after desmopressin but absence of in vitro hypersensitivity to ristocetin. Am J Clin Pathol, 93, 522-525 https://doi.org/10.1093/ajcp/93.4.522
  34. Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocaleau reagent. Methods Enzymol, 299, 152-178
  35. Usenik V, Fabcic J, Stampar F (2008) Sugars, organic acids, phenolic composition and antioxidant activity of sweet cherry (Prunus avium L.). Food Chem, 107, 185-192 https://doi.org/10.1016/j.foodchem.2007.08.004