Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Knock-in Vector for Expression of Insulin-like Growth Factor 1 on the Bovine β-casein Gene Locus

소 β-casein 유전자 영역에서 소 Insulin-like Growth Factor 1을 생산하기 위한 Knock-in Vector

  • Kim, Sang Young (Department of Animal Science, College of Agriculture and Life Science, Chonnam National University) ;
  • Park, Da Som (Department of Animal Science, College of Agriculture and Life Science, Chonnam National University) ;
  • Kim, Se Eun (Department of Animal Science, College of Agriculture and Life Science, Chonnam National University) ;
  • Koo, Deog-Bon (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Kang, Man-Jong (Department of Animal Science, College of Agriculture and Life Science, Chonnam National University)
  • 김상영 (전남대학교 농업생명과학대학 동물자원학부) ;
  • 박다솜 (전남대학교 농업생명과학대학 동물자원학부) ;
  • 김세은 (전남대학교 농업생명과학대학 동물자원학부) ;
  • 구덕본 (대구대학교 공과대학 생명공학과) ;
  • 강만종 (전남대학교 농업생명과학대학 동물자원학부)
  • Received : 2017.07.05
  • Accepted : 2017.08.29
  • Published : 2017.08.31
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Abstract

The production of therapeutic protein from transgenic domestic animal is the major technology of biotechnology. Insulin-like growth factor-1 (IGF-1) is known to play an important role in the growth of the animal. The objective of this study is construction of knock-in vector that bovine IGF-1 gene is inserted into the exon 7 locus of ${\beta}$-casein gene and expressed using the gene regulatory DNA sequence of bovine ${\beta}$-casein gene. The knock-in vector consists of 5' arm region (1.02 kb), bIGF-1 cDNA, CMV-EGFP, and 3' arm region (1.81 kb). To express bIGF-1 gene as transgene, the F2A sequence was fused to the 5' terminal of bIGF-1 gene and inserted into exon 7 of the ${\beta}$-casein gene. As a result, the knock-in vector is confirmed that the amino acids are synthesized without termination from the ${\beta}$-casein exon 7 region to the bIGF-1 gene by DNA sequence. These knock-in vectors may help to create transgenic dairy cattle expressing bovine bIGF-1 protein in the mammary gland via the expression system of the bovine ${\beta}$-casein gene.

Keywords

References

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