Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Antidiabetic Effect of Ethanol Extract of Liriope platyphylla in Streptozotocin Induced Diabetic Rats

Streptozotocin으로 유발된 당뇨쥐에서 맥문동 에탄올 추출물의 당뇨개선효과

  • Kim, Ok-Kyung (Department of Food Science and Nutrition, Dae Jin University)
  • 김옥경 (대진대학교 과학기술대학 식품영양학과)
  • Received : 2017.05.14
  • Accepted : 2017.06.10
  • Published : 2017.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The ethanol extraction yield of Liriope platyphylla(Lp) was about 30.7% by extract apparatus. This study was done to investigate the carbohydrate metabolism related enzyme activities effects of Lp in streptozotocin (STZ)-induced diabetic rats. The contents of serum glucose, triglyceride (TG) were significantly decreaed in Lp treated group compared to the those of STZ-control group, also content of Total cholesterol was decreased. High density lipoprotein (HDL)-cholesterol was increased in Lp treated group. The activity of glucose-6-pase(G-6-Pase) was significantly decreased in Lp treated group. Also the activity of glucokinase(Gk) was increaed in Lp treated group. The content of hepatic glycogen was significantly increaed in Lp treated group. These results indicated that ethanol extract of Lp would have antidiabetic effect in STZ-induced diabetic rats.

본 연구에서는 스트렙토조토신(Streptozotocin,STZ)으로 유발된 당뇨쥐에게 맥문동(Liriope platyphylla,Lp)에탄올 추출물(수율:30.7%)을 1,000mg/kg용량으로 7일간 경구 투여 후 혈청속의 혈당함량과 당대사와 관련된 몇 몇 효소와 지질대사와 관련된 물질을 측정한 결과 혈당과 triglyceride(TG), total cholesterol의 농도는 STZ대조군과 비교하여 맥문동 투여군에서 유의적인 감소를 나타내었고, glucose-6-pase(g-6-pase)활성은 STZ 대조군과 비교하여 유의적인 감소를, glucokinase(gk)활성은 증가를 나타내었다. 또한 hepatic glycogen 함량은 STZ대조군과 비교하여 유의적인 증가를 나타내었다. 따라서 본 실험 결과 맥문동 에탄올 추출물을 스트렙토조토신으로 유발된 당뇨쥐에 있어서 항 당뇨 성분을 함유하고 있음을 알 수 있었다.

Keywords

References

  1. M.H. Kweon, S.T. Kweon, M.S. Ma, Y.I. Park. Lowering effects in plasm cholesterol and body weight by mycellial extract of two mushrooms: agaricus blazai and lentinus edodes. Koean J. Microbial Biotechnol 30:402-409 (2002).
  2. Y.J. Cho, M.A. Bang. hypoglycemic and antioxidative effects of dietary sea-tangle extracts supplementation in streptozotocininduce diabetic rats. The korean of Nutrition 37:5-14(2004).
  3. T.S. Kang, M.S. Kang, J.M. Kang, H.R. Shon, S.Y. Lee. Effect of pleurotus eryngii on the blood glucose and cholesterol in diabetic rats. Koean J. Micology 29:86-90(2001).
  4. S.Y. Kim, W.C. Lee, H.B. Kim, A.J. Kim, S.K. Kim. Antihyperlipidemic effects of methanol extracts from mulberry leaves in cholesterol-induced hyperlipidemia rats. J. Koean Soc. Food Sci. Nutr. 27;1217-1222 (1998).
  5. S.D. Kim, Y.S. Ku, I.Z. Lee, I.K. Park, K.S. Youn. Optimization for hot water extraction condition of Liriope spicata tuber using response surface methodology. Koean J. Postharvest Sci. Technol. 8:157- 163(2001).
  6. S.S. Kang, D.Y. Lee, K.H. Son, J.C. Do. two steroidal saponins from the tubers of Liriope spicaru. Phar. Res. 12:295-299 (1989).
  7. A. Tada, T. Saitoh, J. Shoji. studies on the constituents of Ophiopogonins tuber. VII. Synthetic studies of homoisoflavonoids. Chem. Pharm. Bull 28:2487-2493(1980). https://doi.org/10.1248/cpb.28.2487
  8. M. Tomoda, S. Kato. water soluble carbohydrates of Ophiopogonins tuber. II Purifications properties and structures of three oligosacchsrides. Chem. Pharm. Bull 16 (1968).
  9. I.J. Lee. Effect of Liriopis tuber extract on the decrease of blood glucose Hyosung Bull. Pharm. Sci. 2:49-56(1997).
  10. M.Shibata., R. Noguchi, M. Suzuki, H.I wase, K. Sieda, K. Niwayama, F. Kataoka, K. Kusuda. and M. Hamano. pharmacological studies on medical plant components 1. On the extracts of Ophiopogon and some jolk medicines. Proc. Hoshi. Pharm. 13:66-76(1971).
  11. A. Mita, R. Shida, N. Kasai. and J. Shoji. Enhancement and suppression I production of IgMantibody in mice treated with purified saponins. Biomedicine, 31:223-227(1979).
  12. M. Tomoda. S. Kato. water soluble carbohydrates of Ophiopogoius tuber.II. Purifications propertiesand structuers of three oligosaccharides. Chem. Pharm. Bull. 16:113-116(1968). https://doi.org/10.1248/cpb.16.113
  13. S. Tada. T. Saitoh. J. Shoji. Stuies on the constituents of Ophioponins tuber. VII. Sythetic studies of homoisoflavonoides. Chem. Pharm. Bull. 28:2487-2493(1980). https://doi.org/10.1248/cpb.28.2487
  14. Y.J. Cho, M.A. Bang. Effects of dietary seaweed on bood glucose, lipid and glutation enzymes in streptozotocin induced diabetic rats. J. Korean Soc. Food Sci. Nutr. 33:987-994(2004). https://doi.org/10.3746/jkfn.2004.33.6.987
  15. O.K. Kim. Antidiabetic and Antioidative effect of Lycii fructus in Streptozotocin Induced Diabetic Rats. Kor. J. Pharmacogn. 40 128(2009).
  16. N.Y. Kim, H.K. Jung. Effects of fomes fomentarius extract on blood of pinus densiflora extract on blood glucose level, OGTT and biochemical parameters in streptozotocin induced diabetic rats. J. Korean soc. food sci. nutr.34, 973(2005). https://doi.org/10.3746/jkfn.2005.34.7.973
  17. W.G. Kim, H.J. Kim, Chung M.S., Effects of Germanium-forified tricholoma matsutake mycelium and yeast on blood glucose and serum lipid in streptozotocin induced diabetic rats. Kor. J. Oriental preventive medical society. 13. 89(2009).
  18. H.K. Han, S.J. Yoon, G.H. Kim. Effects of composite plants on plasma glucose and lipid level in streptozotocin induced diabetic rats. J. koreans soc. food sci. nutr. 38, 674(2009). https://doi.org/10.3746/jkfn.2009.38.6.674
  19. M.D. Meglasson, P.T. Burch, D.K. Berner, H. Najafi, F.M, Matschinsky, Identification of glucokinase as an alloxan-sensitive glucose sensor of the pancreatic-cell. Diabetes 35, 1163 (1986). https://doi.org/10.2337/diab.35.10.1163
  20. Jo Y.J., Bang M.A. Effect of dietary seaweed on blood glucose, lipid and glutatione enzymes in streptozotocin induced diabetic tars. J. Korean Soc. Food Sci. Nutr. 33, 987(2004). https://doi.org/10.3746/jkfn.2004.33.6.987
  21. Z. Liu, E.J. Barrett, A.C. Dalkin, A.D. Zwart and J.Y. Chou, Effect of Acute Diabetes on the Rat Hepatic Glucose-6-phosphatase Activity and its Messenger RNA Level. Biochem. Biophy. Res. Commun., 38, 680 (1994).
  22. S. Himeno, A. Takekawa, and N. Imura, Species Diffence in Hydroperoxide Scavenging Enzymes with Special Reference to Glutathione Peroxidase in Guinea-Pigs. Comp. Biochem. Physiol. B, 104, 27 (1993). https://doi.org/10.1016/0305-0491(93)90334-2
  23. A.V. Alabro, : Liver glukinase(A4564) induces potent hypoglycemia without dyslipidemia through a paradoxical induction of the catalytic subunit of glucose-6-phosphatase Int. J. Endocribol., 707928 (2011)
  24. V. Vats, S. P. Yadav, and J. K. Grover, Ethanolic Extract of Ocimum Sanctum Leaves Partially Attenuates Streptozotocin- Induced Alterations in Glycogen Content and Carbohydrate Metabolism in Rats, J. of ethnopharmacology, 90, 155 (2004). https://doi.org/10.1016/j.jep.2003.09.034